Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor

A technology of root-knot nematode and inhibitor, applied in the agricultural field, can solve the problem of no vanillin, etc., and achieve remarkable results

Inactive Publication Date: 2015-03-18
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report of vanillin on the control of root-knot nematode incognita

Method used

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  • Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor
  • Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor
  • Application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the impact of different concentrations of vanillin on the mortality of the second instar larvae of Meloidogyne incognita

[0018] (1) Test material

[0019] 1. Tomato (Lycopersicon esculentum cv.) best powder 19, seeds purchased from Beijing Vegetable and Flower Research Institute.

[0020] 2. Meloidogyne incognita was isolated from a greenhouse in Shouguang, Shandong.

[0021] (2) Cultivation and collection of root-knot nematode incognita

[0022] Planting: Soak Jiafen No. 19 tomato seeds in 15% (V / V) hydrogen peroxide solution for 10 minutes, then wash them with water. Then spread the seeds evenly on the Petri dish covered with two layers of filter paper, and place it in the dark at 28°C. After the root system of the seed is exposed, it is sown in the seedling board, and the vermiculite is used as the substrate for cultivation. Jiafen No. 19 tomato was grown on the seedling board to the three-leaf one-heart stage, and then the seedlings were transpla...

Embodiment 2

[0031] Embodiment 2, the impact of different vanillin concentrations on the hatching of the second instar larvae of Meloidogyne incognita

[0032] Dilute vanillin to a concentration of 0.2mM, 0.4mM, 1mM, 2mM, 4mM, and deionized water (control), soak the egg masses of M. Hatch inhibitory effect of second instar larvae of Meloidogyne incognita.

[0033] Specific investigation method: firstly, select egg masses of uniform size of Meloidogyne incognita and put them into 1.5mL centrifuge tubes, and add 3 egg masses of uniform size to each centrifuge tube. Add 1 mL of insecticidal inhibitors with different concentrations of vanillin to a 1.5 mL centrifuge tube containing 3 egg masses. Cover the cap of the centrifuge tube and place it in a 28°C incubator away from light. Every 24 hours, carefully transfer the solution in the tube to a new 1.5mL centrifuge tube, try not to touch the egg mass at the bottom of the centrifuge tube when drawing the solution, and observe the number of se...

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Abstract

The invention relates to application of vanillic aldehyde in preparation of Meloidogyne incognita Chitwood insecticide inhibitor. The vanillic aldehyde is used as active ingredient of the insecticide inhibitor, the insecticide inhibitor is aqueous solution of the vanillic aldehyde, and the concentration of the vanillic aldehyde in the insecticide inhibitor is 0.2-8mM. The insecticide inhibitor disclosed by the invention is used for preventing and controlling Meloidogyne incognita Chitwood, and the effect is obvious, the lethality to the second-stage juveniles of the Meloidogyne incognita Chitwood is 25.9-88.1%, and relative inhibition ratio to the incubation of the second-stage juveniles can achieve 45.5%-89.9%, and the vanillic aldehyde can be used as a nematode inhibitor in the concentration range.

Description

technical field [0001] The invention relates to the application of vanillin in preparing an insecticidal inhibitor for root-knot nematode incognita, belonging to the field of agricultural technology. Background technique [0002] Incognito root-knot nematode (Meloidogyne incognita) is one of the important pathogenic organisms in the world, it has brought serious loss to the agricultural production in the world, and the economic loss that the whole world causes thus reaches 15.7 billion dollars every year (Abad, P, Gouzy, J, Aury, J.M., et a1. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita. Nat. Biotechnol. 2008, 26, 909-915). And its host range is wide, with a total of more than 1,700 species of plants, including monocots, dicotyledons, herbaceous plants, and woody plants. Among them, vegetable crops of Cucurbitaceae, Solanaceae, and Cruciferae with high economic value, such as tomato, Vegetable crops such as cucumbers, gourds, melons, bitter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/04A01P5/00
Inventor 左元梅钟丽娜董林林李晓林
Owner CHINA AGRI UNIV
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