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A preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin

A technology of cyhalothrin and molecular imprinting, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of low yield, slow mass transfer rate and difficult elution of molecularly imprinted polymers, and achieve strong selective recognition performance , wide detection range, and good monodisperse performance

Inactive Publication Date: 2016-08-24
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to solve the disadvantages of slow mass transfer rate, difficult elution, and low yield of molecularly imprinted polymers prepared by conventional polymerization methods, surface molecular imprinting technology (SMIT) emerged as the times require.

Method used

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  • A preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin
  • A preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin
  • A preparation method of molecularly imprinted material for fluorescent selective recognition of cyhalothrin

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Experimental program
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Effect test

Embodiment 1

[0030] SiO prepared by the Stöver method 2 Nanospheres were added to every 100 mL of ethanol and distilled water at a ratio of 10:3, (v / v) 5.0 mL of ammonia-water mixture, and stirred continuously after ultrasonication for 30 minutes; Aminopropyltriethoxysilane (APTS) was mixed and continuously stirred for 24 hours, and then slowly dropped into the above dispersion liquid. After the dropwise addition, it was kept under room temperature and kept stirring for another 24 hours; after the reaction, ethanol and After being washed several times with distilled water, it was collected by a high-speed centrifuge and dried overnight in a vacuum oven at 40°C to obtain SiO 2 @FITC - APTS microspheres. FTIR as figure 2 shown.

Embodiment 2

[0032] The SiO obtained in 200 mg embodiment 1 2 @FITC-APTS microspheres were ultrasonically dispersed in 70 mL of acetonitrile, and 0.50 mmol of template molecules (cyhalothrin, LC), 2.0 mmol of functional monomers (acrylamide, AM) and 0.4 mL of cross-linking agent (divinyl Benzene, DVB). Self-assembled at room temperature for 12 hours, after the functional monomers and template molecules fully interacted, 2.5% of the initiator azobisisobutyronitrile (AIBN) based on the total amount of the monomers was added. Air was purged by bubbling nitrogen gas for 15 minutes, and sealed under nitrogen atmosphere. Thermally initiated polymerization was used, and it was placed in a constant temperature oil bath at 60°C for 24 h to react. After the polymerization was completed, a pale yellow precipitated polymer was obtained. Soxhlet extraction and elution with a solution of methanol: acetic acid at 9:1 (V / V) to remove cyhalothrin, and then soak the precipitate repeatedly with methanol so...

Embodiment 3

[0034] The SiO obtained in 200 mg embodiment 1 2 @FITC-APTS microspheres were ultrasonically dispersed in 70 mL of acetonitrile, and 0.75 mmol of template molecules (cyhalothrin, LC), 3.0 mmol of functional monomers (acrylamide, AM), and 0.6 mL of cross-linking agent (diethylene phenylene, DVB). Self-assembled at room temperature for 12 hours, after the functional monomers and template molecules fully interacted, 2.5% of the initiator azobisisobutyronitrile (AIBN) based on the total amount of the monomers was added. Air was purged by bubbling nitrogen gas for 15 minutes, and sealed under nitrogen atmosphere. Thermally initiated polymerization was used, and it was heated in a constant temperature oil bath at 60 °C for 24 h. After the polymerization was completed, a pale yellow precipitated polymer was obtained. Soxhlet extraction and elution with a solution of methanol: acetic acid at 9:1 (V / V) to remove cyhalothrin, and then soak the precipitate repeatedly with methanol solu...

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Abstract

The invention relates to a preparation method of a molecularly imprinted material for fluorescent selective recognition of cyhalothrin and belongs to the technical field of environment function material preparation. By the utilization of a precipitation polymerization method, a fluorescent molecularly imprinted polymer is synthesized by using Lambda-cyhalothrin as a template molecule, using SiO2 sub-microspheres as a carrier, using acrylamide (AM) as a functional monomer, using fluorescein isothiocyanate (FITC) as a fluorescent reagent, using divinyl benzene (DVB) as a cross-linking agent and using 2,2'-azodiisobutyronitrile (AIBN) as an initiator. The prepared fluorescent molecularly imprinted polymer has high sensitivity and has high performance of selective recognition of cyhalothrin. Through detection results of a liquor sample, it proves that the material prepared by the method can be used in quantitative detection of practical complex samples.

Description

technical field [0001] The invention relates to a preparation method suitable for fluorescent selective identification and detection of cyhalothrin core-shell type imprinted polymer submicron spheres, and belongs to the technical field of environmental functional material preparation. Background technique [0002] Molecular imprinting technology (MIT) refers to the technology of preparing polymers with certain selective recognition ability. With memory function, polymers synthesized by this technology are called molecular imprinting polymers (MIPs). MIPs prepared by molecular imprinting technology have high selectivity and good affinity for target molecules. Due to the cross-linking agent added in the preparation process, the prepared MIPs have a certain rigid structure, so the properties are relatively stable and the ability to resist harsh environments is strong. At present, MIPs play an important role in more and more fields, and become a research hotspot of interdiscip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/56C08F212/36C08F2/44C08K9/06C08K9/04C08K3/36C08J9/26G01N21/64
Inventor 王吉祥闫永胜高林沈洪强李根刘树成李春香
Owner JIANGSU UNIV
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