Degumming method applicable to green fluorescent protein-containing bave
A technology of green fluorescent protein and degumming method, applied in degumming and other directions, can solve the problems affecting the silk feel, color printing and dyeing processing, use restrictions, poor hand feel of cocoon silk, etc., and achieve the effect of small loss of mechanical properties, good mercerization, and soft hand feeling
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Embodiment 1
[0027] Embodiment 1, green cocoon silk fluorescence observation and gel content determination
[0028] Take 6g of green cocoon silk, put it in a constant temperature oven, and dry it at 105-110°C until it reaches a constant weight, which is the dry weight before the test W 0 , and then the samples were processed under the following conditions:
[0029]
[0030] After the sample is treated under the above conditions, it is fully washed with soft water, and then dried at 105-110 ° C to constant weight, which is the dry weight after the test W 1 , and then calculate the gel content according to the following formula, accurate to 0.01%.
[0031] Glue content = (W 0 -W 1 ) / W 0 ×100%
[0032] Repeat three parallel experiments, and take the average value of three times as the final result of the gel content. The calculated gel content is 26.86%. The glue content is used as the criterion for judging the effect of degumming. The closer the degumming rate is to the glue conten...
Embodiment 2
[0033] Embodiment 2, the method of soap alkali method degumming green cocoon silk
[0034] Take the green cocoon silk sample and dry it in an oven at 105-110°C to constant weight, and record the dry weight of the green cocoon silk as m 0 , soaked for 0.5 hours in a soap solution with a bath ratio of 1:50, wherein the concentration of the soap solution is 3 to 7 g·L -1 Industrial soap and concentration of 1 ~ 5g L -1 Soda ash, then degummed at a temperature of 60-95°C for 20-60 minutes (degumming according to the conditions in Table 1), then washed in water at a temperature of 80°C, 50°C and 18-25°C for 5 minutes, and finally placed Dry in an oven at 105-110°C until constant weight, record the dry weight of degummed cocoons, and denote it as m 1 . According to degumming rate=(m 0 -m 1 ) / m 0 ×100% to calculate the degumming rate, the results are shown in Table 1.
[0035] Table 1. Orthogonal method and results of degumming green cocoon silk by soap alkali method
[0036]...
Embodiment 3
[0038] Embodiment 3, the method for papain degumming green cocoon silk
[0039] Take 9 samples of 2g green cocoon silk, dry them in an oven at 105-110°C to constant weight, and record the dry weight before degumming in m 0 , soaked at 50°C for 0.5h in a pretreatment solution with a bath ratio of 1:50, wherein the pretreatment solution is a solution of pH=10, containing 1g / L soda ash and 0.5g / L penetrant JFC, and then The concentration of papain is 0.5~1g·L -1 , the pH is 5-9, and the temperature is 40-80 ° C under the condition of degumming for 30-60 minutes (degumming according to the conditions in Table 2), followed by washing in water at a temperature of 80 ° C, 50 ° C and 18-25 ° C for 5 minutes, Finally, place it in an oven at 105-110°C and dry it to constant weight, record the dry weight m after degumming 1 . According to degumming rate=(m 0 -m 1 ) / m 0 ×100% to calculate the degumming rate, the results are shown in Table 2.
[0040] Table 2. Orthogonal method and ...
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