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New bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, andacquiring method and application of same

A technology of polycyclic aromatic hydrocarbons and new strains is applied in the field of biological treatment of environmental pollutants, which can solve the problems of unsatisfactory degradation effect of polycyclic aromatic hydrocarbons, and achieve the effect of repairing polluted sites.

Inactive Publication Date: 2015-03-04
HEBEI AGRICULTURAL UNIV.
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  • Summary
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  • Claims
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AI Technical Summary

Problems solved by technology

Most of the bacteria that degrade aromatic hydrocarbons in the past are aimed at low molecular weight PAHs, and the degradation effect on 5-ring and 6-ring PAHs is not ideal, especially for the screening of degrading bacteria that use PAHs-contaminated soil in coal mining areas as a medium. There are reports

Method used

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  • New bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, andacquiring method and application of same
  • New bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, andacquiring method and application of same
  • New bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, andacquiring method and application of same

Examples

Experimental program
Comparison scheme
Effect test

example (1

[0054] Example (1): Degradation of ZH-L8 to benzopyrene monomer

[0055] Inoculate the ZH-H2 single colony preserved after the PAH monomer acclimatization in the above step (4) into LB medium and activate for 48 hours to obtain the enriched bacterial liquid.

[0056] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt liquid medium containing 10mg / benzopyrene as the experimental group, and another control group without enriched bacteria solution, in 150rmp, 30°C shaker Shake culture in the dark for 7 days.

[0057] After the incubation time is over, take 25ml of chromatographically pure n-hexane solution and add it to the Erlenmeyer flasks of the experimental group and the control group, ultrasonically extract for 5 minutes, so that all the PAHs sticking to the wall are soaked into the n-hexane solution, and then the Erlenmeyer flasks are placed in the n-hexane solution. In a 250rmp oscillator, after oscillating for 40 minutes and ultras...

example (2

[0059] Example (2): Degradation of indenopyrene monomer by ZH-L8

[0060] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt medium containing 10mg / L indenopyrene as the experimental group, and another control group without enriched bacteria solution, in 150rmp, 30°C shaker Shake culture in the dark for 7 days, the method of extracting PAHs is the same as example (1), measure the residual rate of polycyclic aromatic hydrocarbons in the solution of the experimental group and the control group respectively, then apply the residual rate of the experimental group and the control group, according to the degradation formula, Calculate the degradation efficiency.

[0061] The experiment was repeated three times, and the results were averaged. The degradation rate of indenopyrene is 42%.

example (3

[0062] Example (3): Degradation of ZH-L8 to dibenzoanthracene monomer

[0063] Inoculate 10% enriched bacteria solution into 20mL sterilized aqueous phase inorganic salt medium containing 10mg / L dibenzanthracene as the experimental group, and another control group without enriched bacteria solution, shake at 150rmp, 30°C Shake culture in medium dark place for 7 days, the method for extracting PAHs is the same as example (1), measure the residual rate of polycyclic aromatic hydrocarbons in the solution of experimental group and control group respectively, apply the residual rate of experimental group and control group then, according to the degradation formula , to calculate the degradation efficiency.

[0064] The experiment was repeated three times, and the results were averaged. The degradation rate of dibenzanthracene was 72%.

[0065] The embodiment of the present invention also provides the application of the above-mentioned new strain to the degradation of the 6-ring p...

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Abstract

The embodiment in the invention provides a new bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, and an acquiring method and application of the same, relates to the technical field of biological processing for environment pollutants, and the new bacterial strain is capable of efficiently degrading the polycyclic aromatic hydrocarbons with five rings or six rings. The new bacterial strain has the name of ZH-L8, the morphological characteristics comprise that a circle milk-white bacterium colony is grown on a solid medium and has a smooth edge; the new bacterial strain is in the shape of a rod when being observed under a microscope; the cell shows a Gram-positive rod shape, the later sides are parallel, and two ends are blunt; and the bacterium colony is circle, the edge is smooth, the bacterium colony is tightly adhered to the medium, and the bacterium colony is white and not transparent. The physiological biochemical characteristics comprise that the bacterium well grows in the temperature scope of 20-45 DEG C, grows well when pH is 5.7 and 6.8, has the salt-resistant concentration of 5%, and is methyl-red positive, contact-enzyme positive, v-p negative with the v-p solution final pH less than 6, arginine utilization positive, nitrate utilization positive, positive for D-glucose acid production, lactose acid production and cane sugar acid production, positive for starch hydrolysis, and the like.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a new bacterial strain for degrading 5-ring and 6-ring polycyclic aromatic hydrocarbons and its acquisition method and application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of toxic organic pollutants containing two or more benzene rings that are widely distributed and stably exist in the natural environment. Because of its "three-cause (carcinogenic, teratogenic, mutagenic) effects, it will also produce phototoxic effects, and it has the characteristics of large hidden dangers, long incubation period, wide coverage, and difficult governance in the soil, especially High-ring PAHs not only have complex chemical structures, high electron cloud density, and are difficult to be oxidized, but also have poor water solubility, strong thermal stability, high solid-water partition coefficient, and low bioava...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 杨志新赵欧亚李玉灵冯圣东石维王伟王小敏李成
Owner HEBEI AGRICULTURAL UNIV.
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