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Method for Concentrating and Separating Catalin from Fermented Broth of Endophytic Fungus of Catalpa and Its Application

A technology of endophytic fungi and fermentation liquid, applied in the field of biochemistry, can solve the problems of temperature sensitivity, low concentration yield, etc., and achieve the effects of preventing pollution, ensuring purity, and safe operation process

Active Publication Date: 2016-08-24
成都上交致远生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, catalin is sensitive to temperature, and operations such as rotary evaporation cause a large amount of catalin to degrade, and reversed-phase adsorption can not effectively elute catalin, resulting in a low concentration yield.

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0030] The preparation of the endophytic fungus fermented liquid of Chinese catalpa comprises the following steps in the following examples:

[0031] 1. Isolation of endophytic fungi: splitting endophytic fungi and purifying them by mycelial tip cutting;

[0032] 2. Induce spore production: Inoculate Trichoderma longibrachiata CCTCC M 208102 on the PDA medium, cut off a piece of the PDA medium with mycelia, put it on the CMX medium, and cultivate it at room temperature. After 1 to 3 days, there will be spores grow;

[0033] 3. Liquid fermentation culture: pick the spores and transfer them to the PDA liquid medium, culture at 26-28° C. on a shaking table at 180 rpm, and stop the fermentation after 1-8 days.

Embodiment 1

[0035] This embodiment relates to a method for concentrating and separating catalpaline from the fermented liquid of endophytic fungus of catalpa, comprising the following steps:

[0036] (1) adjusting the pH of the fermentation broth to be 4;

[0037] (2) The fermentation broth is pre-cooled to 1°C;

[0038] (3) Set the heat transfer temperature difference between the fermentation broth and the cold trap at 4°C to freeze the fermentation broth, add 1 ml of crushed ice at 0°C each time, and add 1 ml of crushed ice at 0°C after the crushed ice completely melts, until the broth starts to freeze;

[0039] (4) Set the heat transfer temperature difference between the fermented liquid and the cold trap to be 2°C and continue to freeze the fermented liquid until the volume of the liquid fermented liquid is reduced to one-tenth of its original volume, achieving 10 times concentration, and then stop freezing.

[0040] Remove the ice cubes, collect the fermented liquid, find that the ...

Embodiment 2

[0043] This embodiment relates to a method for concentrating and separating catalpaline from the fermented liquid of endophytic fungus of catalpa, comprising the following steps:

[0044] (1) adjusting the pH of the fermentation broth to be 8;

[0045] (2) The fermentation broth is pre-cooled to 4°C;

[0046] (3) Set the heat transfer temperature difference between the fermentation broth and the cold trap at 6°C to freeze the fermentation broth, add 1 ml of crushed ice at 0°C each time, and add 1 ml of crushed ice at 0°C after the crushed ice completely melts, until the broth starts to freeze.

[0047] (4) Set the heat transfer temperature difference between the fermented liquid and the cold trap to be 4°C and continue to freeze the fermented liquid until the volume of the liquid fermented liquid is reduced to one fifteenth of its original volume, achieving 15 times concentration, and then stop freezing.

[0048] Remove the ice cubes, collect the fermented liquid, find that ...

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PUM

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Abstract

The invention discloses a method for condensation and separation of catalpa bungei essence Qiulingsu in catalpa bungei endophytic fungus broth and a use thereof. The method comprises the following steps of 1, adjusting a pH value of a catalpa bungei endophytic fungus broth to 4-10, 2, pre-cooling the catalpa bungei endophytic fungus broth subjected to pH adjustment to a pre-cooling temperature of 0-4 DEG C, 3, slowly cooling the catalpa bungei endophytic fungus broth in the case of crystal nucleus existence until the catalpa bungei endophytic fungus broth freezes, and 4, carrying out slow cooling freezing until the rest of the liquid broth is concentrated to a preset volume, stopping cooling and collecting the unfrozen liquid which is catalpa bungei essence Qiulingsu concentrate. The catalpa bungei endophytic fungus broth is concentrated to form the broth with 1 / 10 of the original volume and a catalpa bungei essence Qiulingsu yield is more than 90%. Compared with the prior art, the method effectively guarantees stabilization of catalpa bungei essence Qiulingsu in the raw material, and a high condensation yield, is free of any organic solvent and guarantees concentrate quality.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method for concentrating and separating catalpaline from the fermented liquid of endophytic fungi of catalpa and its application. Background technique [0002] Endophytic fungi are those fungi that live inside plant tissues and do not cause obvious plant diseases. Endophytic fungi can produce the same or similar bioactive substances as the host plant, which has great potential value and can be a source of new anticancer drugs. Our research group has isolated and extracted an endophytic fungus (CCTCC M 208102) with strong anti-cancer activity from Juglans chinensis, and initially identified the fungus as Deuteromycotina, Hyphomycetes, Polychospora, Wood Mold belongs to the genus, and its fermented extract has a strong inhibitory effect on liver cancer cells. This is a brand-new strain unique to our laboratory, and its fermentation broth preparation method with anticancer ability ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07G99/00
Inventor 苗志奇朱闪烁胡郁楠冯婷婷胡丹
Owner 成都上交致远生物科技有限公司
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