A countercurrent inoculation device and inoculation method in solid fermentation

A solid fermentation and countercurrent technology, applied in feed, food science, application, etc., can solve the problems of difficult and thorough cleaning of materials, uneven inoculation, low inoculation production efficiency, etc., to facilitate cleaning and maintenance, and improve inoculation efficiency , the effect of high production efficiency

Active Publication Date: 2016-03-23
张刘运
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of fermentation production is extensive in many enterprises, mainly as follows: 1. The inoculation is carried out in an open space, which is easily polluted by other microorganisms
2. Stirring inoculation, because the moisture of the material is more than 50%, it adheres to the stirring blade, shaft and inner wall, etc., the material is difficult to clean thoroughly, causing secondary pollution
4. The inoculation is uneven, some places have strains, and some places have no strains, so it needs to be stirred again, which increases the production process and production difficulty
5. The uniformity of inoculation and the amount of inoculation cannot be controlled.
6. The temperature during inoculation is significantly lower than the temperature of the bacteria in the storage tank, causing microbial adversity, making the microorganisms in a dormant state, and reducing their vitality
7. The production efficiency of inoculation is low, and the large-scale mass production is limited
In order to solve the above problems, people have carried out long-term research and applied it to production practice, but the results are not satisfactory.

Method used

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  • A countercurrent inoculation device and inoculation method in solid fermentation

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Embodiment 1

[0021] Such as figure 1 As shown, a countercurrent inoculation device in solid fermentation involved in this embodiment includes: a storage bin 1, a strain dilution activation tank 2, a nutrient solution adjustment tank 3, a plunger pump 4, a bacteria liquid nozzle 5, Bacteria tower 6, feeder 7, bulk material blade 8, stirring and oscillating feeder 9, stirring and conveying auger 10, nutrient regulating liquid control valve 11, bulk material blade motor 12, nutrient regulating liquid nozzle 13 and nutrient regulating liquid pipe Road 14. Described waiting bin 1 is arranged on the top of inoculating tower 6, and waiting bin 1 is connected with inoculating tower 6, and the lower end of waiting bin 1 is provided with feeder 7, and the outlet of described feeding device 7 is connected with The feeding port of the bacteria-connecting tower 6 corresponds up and down, and the feeding port of the bacteria-connecting tower 6 is equipped with a bulk material blade motor 12, and the bu...

Embodiment 2

[0031] This embodiment is a method of countercurrent inoculation in solid fermentation. The steps are as follows: 1. Preparation of solid fermentation materials: the solid fermentation materials are removed from impurities, iron and sieved. After mixing evenly, they are crushed and transported to Stand-by in bin 1; 2. Preparation of the bacteria solution to be inoculated: transport the stock solution of the bacteria to be inoculated to a strain dilution and activation tank at a constant temperature of 37°C and add the diluted nutrient solution, and stir evenly for use; 3. The nutrient adjustment solution Preparation: transport the nutrient adjustment liquid used for fermentation to the nutrient liquid adjustment tank 3 for use; 4. Equipment inspection and control adjustment setting: set the spraying amount of the bacterial liquid according to 2% of the weight of the solid fermentation material, press 35% of the weight of the solid fermentation material is used to set the amount...

Embodiment 3

[0033] The countercurrent inoculation method in soybean meal solid fermentation, the steps are as follows: 1. Fermentation material preparation: remove impurities, iron and sieve high-protein soybean meal (protein content greater than 45%) and fermentation auxiliary materials, mix them evenly, and press the process particle size to 40 mesh Requirements, crushed and transported to the waiting bin 1 for use; 2. Preparation of the bacteria solution to be inoculated: transport the original solution of the bacteria to be inoculated GY15 soybean meal to the 37°C constant temperature strain dilution activation tank and add the special bacteria at a ratio of 1:10 Dilute the nutrient solution and stir it evenly for use; 3. Preparation of nutrient adjustment solution: transport the nutrient adjustment solution used for soybean meal fermentation to the nutrient solution adjustment tank 3 according to the composition ratio and mix it for use; 4. Equipment inspection and control adjustment ...

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Abstract

The invention provides a reverse-flow bacteria inoculating device and a reverse-flow bacteria inoculating method in solid fermentation. Material scattering blades in the device are arranged at the upper part of the interior of a bacteria inoculating tower; the output end of a material scattering blade motor is connected with the material scattering blades; a bacteria solution nozzle is arranged at the lower part of the interior of the bacteria inoculating tower; the inlet end of a plunger pump is communicated with the outlet end of a bacteria attenuation activation tank; the outlet end of the plunger pump is communicated with the inlet end of the bacteria solution nozzle; the method is characterized in that bacteria solution is sprayed upwards into foggy drops through a high-pressure pipeline and the bacteria solution nozzle, and the foggy drops are sprayed into the bacteria inoculating tower; while the plunger pump is started, a feeder and the material scattering blade motor are started, and then, a solid fermentation material evenly scatters downwards, and falls to the bottom part of the bacteria inoculating tower after meeting the foggy bacteria solution sprayed reversely and upwards at a high speed. Compared with the traditional method, the reverse-flow bacteria inoculating method provided by the invention is increased by more than 10 times in bacteria inoculating efficiency; bacteria inoculating is performed in the airtight stainless steel bacteria inoculating tower, so that no external contamination is generated; after quantitative feeding of the solid fermentation material and quantitative atomization of the bacteria solution, reverse-flow bacteria inoculating is performed; a high-efficiency airtight and even bacteria inoculating process is realized.

Description

technical field [0001] The invention relates to a countercurrent inoculation device and a method for inoculating bacteria in solid fermentation, and belongs to the technical field of a countercurrent inoculating device and a method for inoculating bacteria. Background technique [0002] Microbial fermentation technology has been widely used in feed production and has made great progress, opening up new channels for animal health and providing new growth points for the development of the feed industry. However, the process of fermentation production is extensive in many enterprises, mainly as follows: 1. The inoculation is carried out in an open space, which is easily polluted by other microorganisms. 2. Stirring inoculation, because the moisture of the material is more than 50%, it adheres to the stirring blade, shaft and inner wall, etc., and the material is difficult to clean thoroughly, causing secondary pollution. 3. Stir again after inoculation to achieve a uniform ino...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23N17/00
Inventor 张刘运李凤刚全立群王德福曹健滨刘新华马光宗秦光远齐虹
Owner 张刘运
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