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Microwave-assisted extraction and enzymatic preparation method of sober-up Corbicula fluminea juice

A microwave-assisted and enzymatic hydrolysis technology is applied in the field of microwave-assisted water extraction and enzymatic hydrolysis to prepare river clam juice with sobering effect. The effect of short time and low energy consumption

Inactive Publication Date: 2015-03-04
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of low efficiency, high energy consumption, long time consumption, high cost and low active ingredient content of clam juice in the technology of hot water extraction of clam juice, and to provide a method for extracting clam juice with sobering effect. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for temporary feeding and spit out sand, after the sand has been spit out, pour them into the cleaning tank for cleaning, weigh the cleaned river clams into a microwave extraction container, According to the ratio of material to liquid 1:2, add water, under the condition of microwave power 500w, 95 ℃, microwave assisted water extraction for 8min, obtain the water extract; Adjust the pH value to 6.2, add 2800U / ml clam juice mixed protease (the composition of the mixed protease is: flavor protease (Flavourzyme): neutral protease (Neutral protease): complex protease (Protamex) = 2:5:3), in Microwave power 700w, under the condition of 50°C, microwave-assisted enzyme hydrolysis for 5 minutes, heating at 100°C for 5 minutes to obtain enzyme hydrolyzate; using polysulfone hollow fiber ultrafiltration membrane with a molecular weight cut-off of 5kDa, with a pressure of 0.4MPa,...

Embodiment 2

[0017] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for temporary feeding and spit out sand, after the sand has been spit out, pour them into the cleaning tank for cleaning, weigh the cleaned river clams into a microwave extraction container, Add water according to the material-to-liquid ratio of 1:1, and under the condition of microwave power 1000w and 88°C, microwave-assisted water extraction for 5 minutes to obtain the water extract; under the condition of vacuum degree of 0.1Mpa, vacuum filter the water extract to obtain the suction filtrate, Adjust the pH value to 6.5, add 2500U / ml clam juice mixed protease (the composition of the mixed protease is: flavor protease (Flavourzyme): neutral protease (Neutral protease): complex protease (Protamex) = 2:5:3), in Microwave power 600w, under the condition of 52°C, microwave-assisted enzyme hydrolysis for 6 minutes, heating at 100°C for 5 minutes to obtain the enzyme hydrol...

Embodiment 3

[0019] Fresh river clams are manually picked to remove shells and other sundries, put them into a holding tank for temporary feeding and spit out sand, after the sand has been spit out, pour them into the cleaning tank for cleaning, weigh the cleaned river clams into a microwave extraction container, Add water according to the ratio of material to liquid 1:3, and under the condition of microwave power 800w and 92°C, microwave-assisted water extraction for 12 minutes to obtain the water extract; under the condition of vacuum degree of 0.1Mpa, vacuum filter the water extract to obtain the suction filtrate, Adjust the pH value to 6.8, add 3000U / ml clam juice mixed protease (the composition of the mixed protease is: flavor protease (Flavourzyme): neutral protease (Neutral protease): complex protease (Protamex) = 2:5:3), in Microwave power 800w, under the condition of 55°C, microwave-assisted enzyme hydrolysis for 8 minutes, heating at 100°C for 5 minutes to obtain the enzyme hydrol...

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Abstract

The present invention discloses a microwave-assisted extraction and enzymatic preparation method of a sober-up Corbicula fluminea juice. The method comprises the following steps: temporarily cultivating Corbicula fluminea, carrying out sand removal, washing, adding water, and carrying out microwave-assisted extraction; carrying out vacuum suction filtration, adjusting pH value of the filtrate, adding proteases, and carrying out microwave-assisted digestion; and performing enzyme inactivation treatment, and separating and concentrating with an ultrafiltering membrane to obtain the sober-up Corbicula fluminea juice. Compared with the existing hot-water extraction method, active ingredients of the Corbicula fluminea are fully extracted by the microwave in the present invention; proteolysis and ultrafiltration separation concentration technology are combined, such that the extraction time is short, the active ingredients content in the Corbicula fluminea is high and sober-up effect is remarkable. The process is simple, efficient, time-saving and yield-increasing. The prepared Corbicula fluminea juice has a significant sober-up effect, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing clam juice with sobering effect by microwave-assisted water extraction and enzymolysis, and belongs to the field of food processing. Background technique [0002] River clam is one of the important economic aquatic products. In my country, river clam has a large output and is extremely rich in resources. It is rich in nutrition, delicious in taste, and has high edible value and health care function. River clams are rich in protein, fat, glycogen, essential amino acids, taurine, multivitamins, calcium, phosphorus, iron, selenium and other minerals with special physiological functions, and also contain physiologically active substances such as active peptides, which have the effect of sobering up Liver protection, heavy metal degradation, anti-fatigue, free radical scavenging, anti-aging and other functions. Clam juice is regarded as a good product for protecting the liver by Chinese people. There is a say...

Claims

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Application Information

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IPC IPC(8): A23L1/221A23L27/10
CPCA23L33/00A23L27/10A23V2002/00A23V2200/334
Inventor 朱小燕李相前顾姝妮
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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