A kind of creatine kinase isoenzyme detection reagent

A technology for detecting reagents and creatine kinase, which is applied in the field of biological reagents, can solve the problems affecting the accuracy of detection results, poor stability of reagents, affecting the application of reagents, etc., and achieves high clinical application value, high reaction activity, and guaranteed accuracy. Effect

Active Publication Date: 2016-03-30
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional reagent detection, the accuracy of the test results is often affected by the presence of pyruvate, heparin and other interfering substances in the sample. At the same time, the anti-CK-M antibody in the reagent is unstable in the reagent, resulting in poor stability of the reagent, which in turn affects the quality of the test results. Application of reagents in the market

Method used

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  • A kind of creatine kinase isoenzyme detection reagent
  • A kind of creatine kinase isoenzyme detection reagent
  • A kind of creatine kinase isoenzyme detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0069] Reagent R1 is composed of the following ingredients:

[0070] Imidazole buffer (pH=6.0) 50mmo1

[0071] Glucose 45mmo1

[0072] Nanoparticles 40mmol

[0073] N-acetylcysteine ​​30mmo1

[0074] Sodium edetate 8mmo1

[0075] Adenosine diphosphate 10mmo1

[0076] coenzyme Ⅰ 2mmo1

[0077] Ribonucleotide 10mmo1

[0078] Pyruvate decarboxylase 0.5KU

[0079] Glucose 6-phosphate dehydrogenase 5KU

[0080] Hexokinase 2KU

[0081] Goat anti-human CK-M polyclonal antibody 50mL

[0082] Trehalose 10g

[0083] Add deionized water to 1L;

[0084] Reagent R2 is composed of the following ingredients:

[0085] Imidazole buffer (pH=6.0) 100mmo1

[0086] Phosphocreatine 120mmo1

[0087] Sodium dodecylbenzenesulfonate 20g

[0088] Sodium azide 10g

[0089] Make up to 1 L with deionized water.

[0090] Nanoparticles for γ-Fe 2 o 3 Nanopart...

Embodiment 2

[0096] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0097] Reagent R1 is composed of the following ingredients:

[0098] Imidazole buffer (pH=6.5) 80mmo1

[0099] Glucose 32mmo1

[0100] Nanoparticles 30mmol

[0101] N-acetylcysteine ​​25mmo1

[0102] Sodium edetate 5mmo1

[0103] adenosine diphosphate 5mmo1

[0104] Coenzyme I 4mmo1

[0105] ribonucleotide 15mmo1

[0106] Pyruvate decarboxylase 1.2KU

[0107] Glucose 6-phosphate dehydrogenase 3KU

[0108] Hexokinase 4KU

[0109] Goat anti-human CK-M polyclonal antibody 30mL

[0110] Trehalose 5g

[0111] Add deionized water to 1L;

[0112] Reagent R2 is composed of the following ingredients:

[0113] Imidazole buffer (pH=7.0) 70mmo1

[0114] Phosphocreatine 170mmo1

[0115] Sodium dodecylbenzenesulfonate 10g

[0116] Proclin3006g

[0117] Make up to 1 L with deionized water.

[0118] Nanoparticles for γ-Fe 2 o 3 Nanoparticles...

Embodiment 3

[0121] A creatine kinase isoenzyme detection reagent is composed of reagent R1 and reagent R2 according to the volume ratio of 4:1;

[0122] Reagent R1 is composed of the following ingredients:

[0123] Imidazole buffer (pH=7.0) 100mmo1

[0124] Glucose 20mmo1

[0125] Nanoparticles 20mmol

[0126] N-acetylcysteine ​​20mmo1

[0127] Sodium edetate 1mmo1

[0128] Adenosine diphosphate 1mmo1

[0129] coenzyme Ⅰ 6mmo1

[0130] Ribonucleotide 25mmo1

[0131] Pyruvate decarboxylase 2KU

[0132] Glucose 6-phosphate dehydrogenase 2KU

[0133] Hexokinase 6KU

[0134] Goat anti-human CK-M polyclonal antibody 10mL

[0135] Trehalose 1g

[0136] Add deionized water to 1L;

[0137] Reagent R2 is composed of the following ingredients:

[0138] Imidazole buffer (pH=6.5) 50mmo1

[0139] Phosphocreatine 220mmo1

[0140] Sodium dodecylbenzenesulfonate 5g

[0141] Proclin3001g

[0142] Make up to 1 L with deionized water.

[0143] Nanoparticles for γ-Fe 2 o 3 Nanoparticles, ...

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Abstract

The invention discloses a creatine kinase isozyme detection reagent. The reagent is composed of reagent R1 and reagent R2 at a ratio of 4:1 by volume, wherein reagent R1 is composed of imidazole buffer, glucose, nanoparticles, N-acetylcysteine, sodium edetate, Adenosine diphosphate, coenzyme Ⅰ, ribonucleotide, pyruvate decarboxylase, 6-phosphate glucose dehydrogenase, hexokinase, goat anti-human CK-M polyclonal antibody and trehalose, reagent R2 is composed of imidazole buffer, creatine phosphate, sodium dodecylbenzenesulfonate and preservatives. The reagent of the invention is a stable, accurate and strong anti-interference reagent for detecting creatine kinase isoenzymes, and has high clinical application value.

Description

technical field [0001] The invention relates to a biological reagent, in particular to a detection reagent, in particular to a creatine kinase isoenzyme detection reagent. Background technique [0002] Creatine Kinase (Creatine Kinase, CK) (ATP: CreatineN-phosphotransferase EC2.7.3.2) usually exists in the cytoplasm and mitochondria of animal heart, muscle, and brain tissues. Purine nucleoside triphosphate (ATP) regeneration is directly related to an important kinase1,2 that reversibly catalyzes the transphosphorylation reaction between creatine and ATP. Creatine kinase has four isozyme forms: muscle (MM), brain (BB), hybrid (MB) and mitochondrial (MiMi). The MM type mainly exists in various muscle cells, the BB type mainly exists in brain cells, the MB type mainly exists in cardiomyocytes, and the MiMi type mainly exists in myocardial and skeletal muscle mitochondria. The muscle creatine kinase molecule is a dimer composed of two identical subunits. According to the prim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/50
CPCG01N33/573
Inventor 范刚王进甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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