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Laser Raman spectrum detection method for quickly analyzing content of basic flavine in soybean hull

A technology of laser Raman spectroscopy and rapid analysis, which is applied in the direction of Raman scattering and material excitation analysis, can solve the problems of time-consuming, expensive liquid chromatography, complicated pre-treatment and instrument operation, and achieve low cost and high reading Intuitive and improved detection sensitivity

Inactive Publication Date: 2015-02-25
河北省食品检验研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the national standard detection method of alkaline yellow in soybean skin is liquid chromatography, and the detection technology reported in domestic and foreign literature is mainly liquid chromatography detection method, but liquid chromatography is expensive, and the pretreatment and instrument operation process is complicated and time-consuming (Takes about 2-3 hours) and other disadvantages

Method used

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  • Laser Raman spectrum detection method for quickly analyzing content of basic flavine in soybean hull

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Experimental program
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Effect test

Embodiment 1

[0031] A laser Raman spectroscopy detection method for quickly analyzing the content of basic yellow in soybean skin, comprising the following steps:

[0032] (1) Determine the characteristic Raman peak of Basic Nenhuang: According to the characteristic chemical structure of Basic Nenhuang, determine the 650cm -1 ±3cm -1 It is the characteristic Raman peak of basic bright yellow.

[0033] (2) Draw the standard curve of Basic Nenhuang: Accurately weigh 0.0100g of the pure Basic Nenhuang, dissolve it in water, and dilute to 100mL to obtain 100μg / mL Basic Nenhuang standard solution, store it in the dark at 4°C, and separate the standard solutions Diluted to 0.2μg / mL, 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 5.0μg / mL serial standard solution, detected by laser Raman spectrometer, the Raman spectrum of the pure product of Basic Nenhuang Baseline adjustment and normalization processing were performed to obtain the basic bright yellow standard curve.

[0034] (3) Preparation of sample soluti...

Embodiment 2

[0052] A laser Raman spectroscopy detection method for quickly analyzing the content of basic yellow in soybean skin, comprising the following steps:

[0053] (1) Determine the characteristic Raman peak of Basic Nenhuang: According to the characteristic chemical structure of Basic Nenhuang, determine the 650cm -1 ±3cm -1 It is the characteristic Raman peak of basic bright yellow.

[0054] (2) Draw the standard curve of Basic Nenhuang: Accurately weigh 0.0100g of the pure Basic Nenhuang, dissolve it in water, and dilute to 100mL to obtain 100μg / mL Basic Nenhuang standard solution, store it in the dark at 4°C, and separate the standard solutions Diluted to 0.2μg / mL, 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 5.0μg / mL serial standard solution, detected by laser Raman spectrometer, the Raman spectrum of the pure product of Basic Nenhuang Baseline adjustment and normalization processing were performed to obtain the basic bright yellow standard curve.

[0055] (3) Preparation of sample soluti...

Embodiment 3

[0073] A laser Raman spectroscopy detection method for quickly analyzing the content of basic yellow in soybean skin, comprising the following steps:

[0074] (1) Determine the characteristic Raman peak of Basic Nenhuang: According to the characteristic chemical structure of Basic Nenhuang, determine the 650cm -1 ±3cm -1 It is the characteristic Raman peak of basic bright yellow.

[0075] (2) Draw the standard curve of Basic Nenhuang: Accurately weigh 0.0100g of the pure Basic Nenhuang, dissolve it in water, and dilute to 100mL to obtain 100μg / mL Basic Nenhuang standard solution, store it in the dark at 4°C, and separate the standard solutions Diluted to 0.2μg / mL, 0.5μg / mL, 1.0μg / mL, 2.0μg / mL, 5.0μg / mL serial standard solution, detected by laser Raman spectrometer, the Raman spectrum of the pure product of Basic Nenhuang Baseline adjustment and normalization processing were performed to obtain the basic bright yellow standard curve.

[0076] (3) Preparation of sample soluti...

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Abstract

The invention discloses a laser Raman spectrum detection method for quickly analyzing content of basic flavine in soybean hull and relates to the technical field of food detection. The laser Raman spectrum detection method includes the following steps: (1), determining basic flavine characteristic Raman peak: 650cm-1+ / -3cm-1; (2), drawing a basic flavine standard curve; (3), preparing a to-be-detected sample solution; (4), taking the to-be-detected sample solution, adding nano enhanced reagent and inorganic salt flocculant, rocking uniformly, using a laser Raman spectrum instrument to detect, and acquiring a Raman spectrogram of a sample, wherein the nano enhanced reagent is one of gold, silver or copper nano sol; (5), performing qualitative determination; (6), performing quantitative determination. The laser Raman spectrum detection method has the advantages of being simple in operation and accurate, quick, lossless and low-cost in detection, is suitable for on-site emergency detection, enterprise self-control detection, large-scale sample screening detection and the like, can be used for both qualitative analysis and quantitative analysis, problematic food can be effectively recognized, and consumer rights and interests can be protected.

Description

technical field [0001] The invention relates to the technical field of food detection. Background technique [0002] Soybean skins should be made from soybeans of the same year, sorted and sieved, and then crushed, refined, filtered, and evaporated to dryness. However, the places where some unscrupulous traders produce bean curds usually have extremely poor sanitation conditions, and low-quality starch and illegal additives are added to soybean milk, so the color of the produced bean curds is not good. To improve the sensory index of bean curds, manufacturers will add Some non-food colorings, such as basic orange, basic bright yellow, etc. Basic bright yellow is a yellow powder, an industrial dye, mainly used for dyeing linen, paper, leather, straw woven products, rayon, etc., and also used for printing and dyeing cotton products. It has a mild stimulating effect on the skin and mucous membranes, and can cause dermatitis, conjunctivitis and upper respiratory tract irritati...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 蔡立鹏张岩汪泓李挥郭浔张丽冰王亮张永辉王洪涛张雷雷
Owner 河北省食品检验研究院
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