Method for identifying age of ginseng by utilizing length of ginseng telomere
A technology of ginseng and age, which is applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problem that the identification and quantification of ginseng age cannot be recognized by others
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Embodiment 1
[0048] Example 1. Establishment of a method for identifying the age of ginseng by using ginseng telomere length
[0049] Ginseng tested: ginseng samples of different years from two regions, see Table 1 for details.
[0050] Table 1 Ginseng samples of different years in two regions
[0051]
[0052] 1. Extraction of Ginseng Genomic DNA
[0053] Genomic DNA of each tested ginseng was extracted by CTAB method. details as follows:
[0054] Take 10-50 mg dry weight (without mildew) of the ginseng to be tested located 1-2 cm below the reed head, put it in a pulverizer, grind it, and pass it through a 40-mesh sieve. Transfer the powder to a 2.0mL Eppendorf tube, add 900μL of sterilized CTAB extract (recipe: 2% (2g / 100ml) CTAB, 100mmol / L Tris-HCl pH=8.0, 20mmol / L EDTA, 1.4mol / L NaCl), 0.02g PVP 40000, 10μL β-mercaptoethanol, fully vortexed and mixed, 65°C water bath for 1.5h-2h, and gently shaked 2-3 times during the period. After completion, take it out and cool to room temp...
Embodiment 2
[0076] Example 2, the application of the method of identifying the age of ginseng by using the telomere length of ginseng
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