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Establishment method of primary liver cancer mouse model

A primary liver cancer and mouse model technology, applied in the direction of active ingredients such as amines, can solve the problems of long establishment time, death, high feeding cost, etc., and achieve the effect of short establishment cycle, good repeatability, and short feeding cycle

Active Publication Date: 2015-02-18
HEILONGJIANG HEIKE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems of long establishment time, high feeding cost, and easy death during feeding in the existing method for establishing the mouse model of primary liver cancer, and provides a method for establishing the mouse model of primary liver cancer

Method used

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  • Establishment method of primary liver cancer mouse model
  • Establishment method of primary liver cancer mouse model
  • Establishment method of primary liver cancer mouse model

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Experimental program
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specific Embodiment approach 1

[0015] Specific embodiment one: the establishment method of primary liver cancer mouse model of this embodiment, carry out according to the following steps:

[0016] 1. Select 4-week-old ICR male mice and observe them for 2 weeks;

[0017] Two, after raising for 2 weeks, measure the body weight of each mouse and the amount of drinking water every day;

[0018] 3. Disinfect the abdomen of the rat with alcohol cotton with a volume concentration of 75%, inject DEN aqueous solution into the abdominal cavity, and start to allow the rat to drink water containing 0.15% DEN by volume after 12 hours, and feed for 4 weeks;

[0019] 4. After 4 weeks, intraperitoneal injection of DEN aqueous solution again, and then feeding, free drinking water during feeding, water containing 0.15% DEN by volume;

[0020] 5. Calculated from the first injection of DEN aqueous solution, the mouse model of primary liver cancer was obtained after feeding until the 10th week.

specific Embodiment approach 2

[0021] Embodiment 2: This embodiment differs from Embodiment 1 in that: in step 3, the DEN injection dose for each mouse is 200 mg of DEN injected per kg body weight of the mouse. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0022] Embodiment 3: This embodiment differs from Embodiment 1 in that: in step 4, the DEN injection dose for each mouse is 200 mg of DEN injected per kg body weight of the mouse. Others are the same as in the first embodiment.

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Abstract

The invention provides an establishment method of a primary liver cancer mouse model and relates to an establishment method of a liver cancer mouse model. The invention solves the problems of an existing establishment method of the primary liver cancer mouse model that the establishment time is long, the feeding cost is high, and the death easily occurs in feeding. The establishment method comprises the following steps of: 1, selecting a 4-week-old ICR (Institute for Cancer Research) male mouse, and observing and feeding the mouse for 2 weeks; 2, after the male mouse is fed for 2 weeks, determining the body weight of each mouse and the daily water intake; 3, disinfecting the abdomen of the mouse by using alcohol cotton with a volume concentration of 75 percent, injecting DEN (Diethylnitrosamine) aqueous solution into an abdominal cavity, enabling the mouse to drink water containing DEN with a volume concentration of 0.15 percent after 12 hours and feeding for 4 weeks; 4, after 4 weeks, injecting the DEN aqueous solution into the abdominal cavity again, then feeding and drinking the water containing the DEN freely during the feeding period; 5, feeding to 10 weeks as of the day of injecting the DEN aqueous solution for the first time to obtain the primary liver cancer mouse model. The establishment method is also used for establishing an in-situ liver cancer animal model.

Description

technical field [0001] The invention relates to a method for establishing a mouse model of liver cancer. Background technique [0002] Primary liver cancer is one of the malignant tumors with a high incidence rate in my country. In recent years, the incidence rate of liver cancer has been on the rise, accounting for the second place among malignant tumors in my country. The occurrence and development of liver cancer is the result of multiple factors. At present, the pathogenic mechanism of liver cancer has not been studied clearly, and there is no safe and effective treatment. Therefore, an in-depth study of the pathogenesis is an important basis for the treatment of liver cancer. Experimental animal cancer models are models established in human cancer research that can simulate the performance of human cancer. Therefore, using the mouse model to study the pathogenesis and pathogenic mechanism of liver cancer is an important means of liver cancer research. [0003] The est...

Claims

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Application Information

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IPC IPC(8): A61K31/15
CPCA61K31/15
Inventor 曹涤非黄鑫华吴琼王雷王东凯孙尧薛佳莹李瑶黄国庆赵金海侯婉滢邢言
Owner HEILONGJIANG HEIKE TECH CO LTD
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