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Utilization method of threonine mycoprotein

A technology of bacterial protein and threonine, applied in the field of threonine production, can solve the problems of decreased pork quality and taste, unable to meet the requirements of healthy diet, etc., and achieve the effects of improving immunity, increasing appetite, and promoting metabolism

Active Publication Date: 2015-02-18
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Residues of antibiotics, growth hormones and other drugs in pig feed have significantly reduced the quality and taste of pork, and cannot meet people's requirements for dietary health

Method used

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  • Utilization method of threonine mycoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A threonine bacterial protein utilization method, which comprises the following steps:

[0035] Step 1) Separation of bacterial protein: the threonine fermentation broth enters a high-speed disc separator for separation at a speed of 5000r / min, and centrifuges for 10 minutes to collect the upper liquid and bacterial protein; filter the upper liquid with an ultrafiltration membrane to collect the concentrated liquid and Permeate: pump the permeate into the decolorization tank for decolorization treatment, add activated carbon accounting for 1% of the permeate mass in the decolorization tank, control the temperature in the decolorization tank to 45°C, and remove the activated carbon by plate and frame filtration to obtain the feed liquid after decolorization for 30 minutes A; Among them, the molecular weight cut-off of the ultrafiltration membrane is 300MW, and the ultrafiltration temperature is 40°C;

[0036] Step 2) Separation of crystals and mother liquor: feed liquid ...

Embodiment 2

[0047] A threonine bacterial protein utilization method, which comprises the following steps:

[0048]Step 1) Separation of bacterial protein: the threonine fermentation broth enters a high-speed disc separator for separation at a speed of 4000r / min, and centrifuges for 15 minutes to collect the upper liquid and bacterial protein; filter the upper liquid with an ultrafiltration membrane to collect the concentrated liquid and Permeate: pump the permeate into the decolorization tank for decolorization treatment, add activated carbon accounting for 2% of the permeate mass in the decolorization tank, control the temperature in the decolorization tank to 45°C, and remove the activated carbon by plate and frame filtration to obtain the feed liquid after decolorization for 20 minutes A; Among them, the molecular weight cut-off of the ultrafiltration membrane is 300MW, and the ultrafiltration temperature is 40°C;

[0049] Step 2) Separation of crystals and mother liquor: feed liquid A...

Embodiment 3

[0060] Feed effect test prepared by the present invention:

[0061] 1. Select 90 swine fever-infected pigs, aged between 2 and 4 months; the symptoms are: unknown high fever, loss of appetite or disappearance, conjunctivitis with purulent secretions, and purulent mucus often flow out of the nasal cavity , with vomiting and blood in the stool; there are often many spot-like bleeding or large red spots on the skin of the base of the ear, abdomen, and medial thigh. Mesenteric lymph nodes are swollen, often with hemorrhagic enteritis, and button ulcers in the mucosa of the large intestine. They were randomly divided into three groups, 30 in each group; all three groups were treated with Qingwenbaidu powder, 20g per 1kg of body weight, for 7 consecutive days.

[0062] Experimental group: given the feed prepared in Example 1 of the present invention; Control group 1: given Tongwei medium and large pig compound feed; Control group 2: feed prepared according to the method of Example ...

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Abstract

The invention belongs to the technical field of threonine production, and discloses a utilization method of threonine mycoprotein. The utilization method of the threonine mycoprotein comprises the following steps: 1) separating the mycoprotein; 2) separating crystals and mother liquor; 3) hydrolyzing the mycoprotein; 4) preparing composite powder; 5) preparing main materials; 6) preparing feed. By the utilization method of the threonine mycoprotein, the mycoprotein is added into the feed, so that the production cost of the feed is reduced and wastes are turned into treasures; antibiotics are not added, so that the edible safety of meat products is ensured.

Description

[0001] technical field [0002] The invention belongs to the technical field of threonine production, and in particular relates to a threonine cell protein utilization method. [0003] Background technique [0004] L-threonine is an essential amino acid that constitutes human, animal and plant proteins, and is mainly used in medicine, chemical reagents, food fortifiers, feed additives, etc. According to relevant statistics, the world's threonine production was only 4,000 tons in 1993, increased to 25,000 tons in 1999, exceeded 40,000 tons in 2002, and now exceeds 200,000 tons. The rapid development of threonine reflects its importance in the field of amino acid fermentation. Most of threonine production uses corn steep liquor and molasses as fermentation nitrogen source, threonine fermentation bacteria are directly extracted from threonine after membrane filtration, fermentation by-product bacterial protein is filtered through flocculation sedimentation plate frame, and di...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/16
CPCY02P60/87
Inventor 董力青冯珍泉丁兆堂郭永波
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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