Gerbera piloselloides extractive, as well as preparation method and application thereof
The technology of Gerbera gerbera and its extract is applied in the field of gerbera gerbera extract and its preparation and application, and can solve the problems of plant phytotoxicity and drug resistance, affecting the application of anti-phytopathogenic fungi preparations, etc.
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[0087] Embodiment 1, the preparation of gerbera officinalis extract and its experiment of inhibiting phytopathogenic fungus
[0088] 1. Preparation of Gerbera officinalis extract
[0089] This step prepared the following eight kinds of gerbera extracts: 95% ethanol extract of gerbera, petroleum ether extract of gerbera, ethyl acetate extract of gerbera, water soluble matter of gerbera, ethyl acetate of gerbera-petroleum ether= 9% eluate, ethyl gerbera acetate-petroleum ether=20% eluate, ethyl gerbera acetate-petroleum ether=50% eluate, ethyl gerbera acetate eluate. The specific preparation method is as follows:
[0090] 1) Grinding and pulverizing the whole gerbera in liquid nitrogen, adding 95% ethanol aqueous solution to extract, collecting the extract, concentrating under reduced pressure to remove the ethanol in the extract to obtain an extract, which is Gerbera 95 % ethanol extract;
[0091] 2) disperse the gerbera 95% ethanol extract of step 1) into a suspension with ...
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