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Method for inducing sugarcane embryoids by spermine

An embryoid body and sugarcane technology, which is applied in the tissue culture field of plant biotechnology, can solve problems such as failure to retrieve spermine, and achieve the effects of low production cost, high speed and good quality.

Inactive Publication Date: 2015-02-18
GUANGXI UNIV
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Problems solved by technology

However, no research data on the use of spermine to induce sugarcane embryoid bodies has been found

Method used

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  • Method for inducing sugarcane embryoids by spermine

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[0017]

[0018] Note: Spm. in the attached table is spermine, an innovative sugarcane embryoid inducer of the present invention.

[0019] The incidence of embryoid bodies (%)=the number of callus masses that re-differentiated into embryoid bodies (regardless of the number of embryoid bodies) / the number of inoculated callus masses×100%, which is one of the quality indicators of callus.

[0020] "The number of re-differentiated embryoid bodies that can be distinguished by the naked eye" refers to the total average number of embryoid bodies that have turned green (indicating chloroplast re-differentiation), leaves and seedlings that can be discerned by the naked eye at the end of the culture period It is an important quality indicator for embryoid bodies.

[0021] From the comparison of Example 1 and Example 2 in the attached table, and the comparison between Example 3 and Example 4, we can see that under the premise of ensuring that the medium has sufficient nutrients to supply the cul...

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Abstract

The invention relates to a method for inducing sugarcane embryoids by spermine. The method takes a young sugarcane leaf section as an explant, and comprises the steps of enabling the young sugarcane leaf section to be inoculated into a solid medium of 2.5mg / L of MS spermine (Spm.) with the pH value of 5.8-6.0, culturing for 20 days and inducing to obtain sugarcane callus agglomerates; under the condition of no change, continuously culturing for 15 days and 20 days to successively obtain embryoids with various shapes; or transferring the agglomerates which are cultured for 20 days and are induced to have callus into a fresh original medium, and culturing for 10 days and 15 days to obtain a great deal of embryoids. The method is high in induction speed, high in efficiency and good in quality when being used for inducing the sugarcane embryoids; the obtained sugarcane embryoids easily generate organs such as roots and leaves by continuous redifferentiation, and complete regeneration plants can be obtained; the total effect of the method is superior to the traditional technology for inducing the sugarcane embryoids by 2, 4-D and the new technology for inducing the sugarcane embryoids by LFS; the method is lower in production cost.

Description

Technical field [0001] The invention belongs to the technical field of tissue culture of plant biotechnology, in particular to a method for inducing sugarcane embryoid bodies with spermine. technical background [0002] "Embryonic body" is a part of the parenchyma cells in the callus that do not undergo sexual reproduction, and directly re-differentiate to form a structure with the function of an embryo. It is also called "somatic embryo" or "somatic embryo" to distinguish it from "Zygotic embryo". "Embryoids" can be directly used in the production of artificial seeds and the production of regenerated plants (test tube seedlings); in theoretical research, it is to explore cell differentiation, organ formation, individual development, and related anatomical, physiological and biochemical changes Important material for the process. Therefore, how to obtain embryoid bodies is one of the hot technical fields concerned by the botanical community. There have been some reports on the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 许鸿源周凤珏蓝桃菊龚银花梁琼月许皓翔
Owner GUANGXI UNIV
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