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Lactobacillus paracasei direct-fed type quantitative detection method for mold and yeast in bacteria

A qualitative detection, Lactobacillus technology, applied in the field of microbiological inspection, can solve the problems of dilution factor, inhibition test result error, etc., and achieve the effect of avoiding drum cover quality problems, avoiding interference, and ensuring stability

Active Publication Date: 2015-02-04
YUNNAN HUANGSHI LESSON DAIRY IND
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] In view of the above-mentioned deficiencies existing in the prior art, the purpose of the present invention is to provide a simple and practical method for the qualitative detection of mold and yeast in the Lactobacillus paracasei direct injection strain, mainly to solve the problem of directly using the national standard method to detect the dilution factor, The interference of factors such as inhibition will cause a large error in the test results

Method used

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  • Lactobacillus paracasei direct-fed type quantitative detection method for mold and yeast in bacteria
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  • Lactobacillus paracasei direct-fed type quantitative detection method for mold and yeast in bacteria

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Embodiment 1

[0015] A kind of qualitative detection method of mold and yeast in Lactobacillus paracasei direct-throwing type bacterial classification, comprises the processing step of following order:

[0016] (1) Preparation of samples: filter on a 100-grade clean bench, weigh 5g of Lactobacillus paracasei direct-injection strain into a conical flask filled with 95g of sterilized saline, and vibrate with a slapping shaker 2min, stand in a sterile environment for 30min, and prepare a sample homogeneous solution with a mass ratio of 1:20;

[0017] (2) Preparation of medium: heat to dissolve commercial Bengal red medium, sterilize with high-pressure steam at 121°C for 15 minutes, after sterilization, when the medium is cooled to 45-50°C, pour it on a sterilized plate, the thickness of the medium is 5mm , solidified for use;

[0018] (3) The first suction filtration: First, use sterile tweezers to pick up the edge of the sterile filter membrane, put the rough side up, and stick it on the ste...

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Abstract

The invention discloses a lactobacillus paracasei direct-fed type quantitative detection method for mold and yeast in bacteria, belonging to the technical field of microorganism verification. The detection method comprises the following verification steps in sequence: (1) preparing a sample, namely, preparing a uniform sample solution with the ratio of 1:20; (2) preparing a culture medium; (3) performing suction filtration for the first time, and pasting a filtered filter membrane on a prepared rose-bengal agar plate; (4) performing suction filtration for the second time, namely, diluting the filter membrane obtained from the first suction filtration in 20-40g of sterile saline solution, performing suction filtration for the second time, and pasting another filtered filter membrane on another prepared rose-bengal agar plate; (5) performing suction filtration for the third time; and (6) observing the result, namely, culturing the plate for 5 days at 28+ / -1 DEG C, and observing the growth situation of bacterial colonies on the filter membrane. The invention mainly aims at solving the problem that the detection result has great errors as an international method which is directly used can be interfered by factors such as the dilution ratio and the inhibition function.

Description

technical field [0001] The invention belongs to the technical field of microbiology inspection, and in particular relates to a method for detecting mold and yeast in Lactobacillus paracasei direct injection strains. Background technique [0002] Directed Vat Set (DVS) refers to a series of highly concentrated and standardized freeze-dried starter cultures, which can be directly added to heat-treated raw milk for fermentation without activation and expansion. And other preprocessing work. The number of viable bacteria in the direct-throwing starter is generally 10 10 -10 12 CFU / g, due to the strong vitality and various types of direct-into-type starters, manufacturers can choose arbitrarily according to their needs, thus enriching the variety of lactic acid bacteria products. The direct investment starter does not need to be expanded and cultivated, it can be used directly, and the product quality is relatively stable, so it has become the first choice for large dairy comp...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/645
Inventor 董雪凤杨子彪赵旭燕
Owner YUNNAN HUANGSHI LESSON DAIRY IND
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