Method for maintaining activity of bone morphogenetic protein-2 under irradiation sterilization condition

A technology of activity and radiation dose, applied in the field of activity maintenance, can solve the problem of BMP-2 inactivation and other problems, achieve the effect of solving inactivation and improving application prospects

Inactive Publication Date: 2015-01-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, several current conventional sterilization methods will inactivate BMP-2

Method used

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  • Method for maintaining activity of bone morphogenetic protein-2 under irradiation sterilization condition
  • Method for maintaining activity of bone morphogenetic protein-2 under irradiation sterilization condition
  • Method for maintaining activity of bone morphogenetic protein-2 under irradiation sterilization condition

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1. Prepare the BMP-2 solution and add it to a centrifuge tube, seal it with a parafilm, put it into a thermos bottle, fill it with ice, and use 60 Co source ray irradiation, the set dose is 0.01kGy.

[0018] 2. The umbilical cord blood mesenchymal stem cells were routinely cultured in DF12 complete culture medium, and the non-irradiated BMP-2 group and the irradiated BMP-2 group were set up respectively. After 7 days of cell culture, the ALP content of each well was detected.

[0019] 3. Through the analysis of ALP content, we can see that after BMP-2 is irradiated, its ALP expression level is similar to that of non-irradiated BMP-2, and there is no significant difference, thus proving that after irradiation BMP-2 activity was maintained.

Embodiment 2

[0021] 1. Prepare the BMP-2 solution and add it to a centrifuge tube, seal it with a parafilm, put it into a thermos bottle, fill it with ice, and use 137 Cs source ray irradiation, the set dose is 0.01kGy.

[0022] 2. The umbilical cord blood mesenchymal stem cells were routinely cultured in DF12 complete culture medium, and the non-irradiated BMP-2 group and the irradiated BMP-2 group were set up respectively. After 7 days of cell culture, the ALP content of each well was detected.

[0023] 3. Through the analysis of ALP content, we can see that after BMP-2 is irradiated, its ALP expression level is similar to that of non-irradiated BMP-2, and there is no significant difference, thus proving that after irradiation BMP-2 activity was maintained.

Embodiment 3

[0025] 1. Prepare the BMP-2 solution and add it to a centrifuge tube, seal it with a parafilm, put it into a thermos bottle, fill it with ice, and use 192 The Ir source ray is irradiated, and the set dose is 0.01kGy.

[0026] 2. The umbilical cord blood mesenchymal stem cells were routinely cultured in DF12 complete culture medium, and the non-irradiated BMP-2 group and the irradiated BMP-2 group were set up respectively. After 7 days of cell culture, the ALP content of each well was detected.

[0027] 3. Through the analysis of ALP content, we can see that after BMP-2 is irradiated, its ALP expression level is similar to that of non-irradiated BMP-2, and there is no significant difference, thus proving that after irradiation BMP-2 activity was maintained.

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Abstract

The invention relates to a method for maintaining the activity of bone morphogenetic protein-2 under an irradiation sterilization condition. The method comprises the following steps of adding a BMP-2 solution into a centrifugal pipe, sealing by a sealing membrane, putting into a thermal insulation bottle, filling with ice, irradiating by 60Co, 137Cs, 192Ir source rays, setting the dosage to be 0.01-100kGy, and carrying out the irradiation process of BMP-2. The method has the advantages that the inactivation problem in the BMP-2 irradiation process is solved effectively, the research development of medical treatment equipment for loading BMP-2 is pushed, and the application prospect is improved greatly.

Description

technical field [0001] The present invention relates to the activity maintenance of bone morphogenetic protein-2 (BMP-2) in the radiation sterilization process, and its irradiation object is BMP-2, adopts 60 Co. 137 Cs, 192 Ir emission source, the irradiation dose is 0.01-100kGy. Background technique [0002] Bone morphogenetic protein (BMP) is widely used mainly because of its ability to induce bone and cartilage formation, as a therapeutic agent for the treatment of fractures and periodontal defects, and for its ability to induce bone growth around implants and artificial prostheses. Traditional gene knockout experiments have shown that bone morphogenetic proteins have a variety of biological activities, such as early embryogenesis, multi-faceted organ formation, etc., and can achieve functions by regulating cell proliferation, differentiation and apoptosis. Through in vitro and in vivo experiments, a series of human bone morphogenetic proteins have been confirmed to st...

Claims

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Application Information

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IPC IPC(8): A61L2/08
CPCA61L2/08
Inventor 亓洪昭杨贤金卢云峰李朝阳李雪崔振铎原续波王思颖汪亚运
Owner TIANJIN UNIV
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