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Method for detoxifying lily based on embryogenic callus specific induction

A technology of embryogenic callus and embryogenic callus, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of large demand for detoxification materials, poor resistance, and easy pollution of stem tip disinfection, etc. Achieve the effect of being suitable for promotion and use, avoiding the disinfection process, and having a good detoxification effect

Active Publication Date: 2015-01-28
龙山县喜乐百合食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The problems of this detoxification method are: the demand for detoxification materials is large, the disinfection of the stem tip is easy to pollute, the stem tip is easily browned after stripping, and the detoxification bulbs formed have serious degradation of species and poor resistance, although they can remove part of the virus. , but in the process of multiplication and cultivation, the growth of the plants is poor, and it is easy to be infected with the virus again, so it is difficult to apply to the actual production
In addition, the Chinese patent No. CN201210050972.2 "Oriental Lily Seed Bulb Detoxification Method" has undergone heat treatment, stem tip stripping, chemical detoxification and other methods, but has not undergone callus detoxification and tissue culture seedlings. Peeling the stem tip can easily cause severe degradation of the species, and in this method, the stem tip stripping is not easy to operate, so it is difficult to form virus-free seedlings for large-scale production

Method used

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  • Method for detoxifying lily based on embryogenic callus specific induction

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Embodiment 1

[0040] A lily detoxification method based on the specific induction of embryogenic callus, the detailed steps of the lily detoxification method are as follows:

[0041] 1) Specific induction of embryogenic callus from lily scales free of diseases and insect pests through tissue culture: select intact lily scales without disease spots, wash them with clean water, then soak them in neutral soapy water for 15 minutes, and then rinse them with running water 1 hour; soak in 75% alcohol for 40 seconds, put in 0.2% mercuric chloride for 10 minutes, rinse with sterile water 5 times, and then cut the scales into 0.5cm 2 Put the cubes into the medium for inducing embryogenic callus for dark culture, transfer once after 30 days, and form embryogenic callus after continuing to culture for 30 days, which is a medium for inducing embryogenic callus from lily scales It is: MS+2mg / L6-BA+1mg / L2, 4-D+6% sucrose+6.5g / L agar, the pH of the medium is 5.8;

[0042] 2) Induce embryogenic callus to ...

Embodiment 2

[0047] A lily detoxification method based on the specific induction of embryogenic callus, the detailed steps of the lily detoxification method are as follows:

[0048] 1) Specific induction of embryogenic callus from lily scales free of diseases and insect pests through tissue culture: select intact lily scales without lesions, wash them with clean water, then soak them in neutral soapy water for 20 minutes, and then rinse them with running water 2 hours; soak in 75% alcohol for 30s, put in 0.1% mercuric chloride for 15 minutes, rinse with sterile water 4 times, and then cut the scales into 1.0cm 2 Put the cubes into the medium for inducing embryogenic callus for dark culture, transfer once after 25 days, and continue to culture for 35 days to form embryogenic callus, which is a medium for inducing embryogenic callus from lily scales It is: MS+2mg / L6-BA+1mg / L2, 4-D+6% sucrose+6.5g / L agar, the pH of the medium is 5.8;

[0049] 2) Induce embryogenic callus to form seedlings an...

Embodiment 3

[0054] A lily detoxification method based on the specific induction of embryogenic callus, the detailed steps of the lily detoxification method are as follows:

[0055] 1) Specific induction of embryogenic callus from lily scales free of diseases and insect pests through tissue culture: select intact lily scales without disease spots, rinse them with clean water, then soak them in neutral soapy water for 18 minutes, and then rinse them with running water 0.5 hours; Soak in 75% alcohol for 35s, put in 0.15% mercuric chloride for 13 minutes, rinse with sterile water 6 times, and cut the scales into 0.8cm 2 Put the cubes into the medium for inducing embryogenic callus for dark culture, transfer once after 35 days, and continue to culture for 25 days to form embryogenic callus, which is a medium for inducing embryogenic callus from lily scales It is: MS+2mg / L6-BA+1mg / L2, 4-D+6% sucrose+6.5g / L agar, the pH of the medium is 5.8;

[0056] 2) Induce embryogenic callus to form seedlin...

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Abstract

A disclosed method for detoxifying lily based on embryogenic callus specific induction is characterized by comprising the following steps: 1) performing tissue-culture specific induction on lily scale free of insect diseases, so as to generate embryogenic callus; 2) inducing the embryogenic callus to form a seedling and performing heat processing; 3) peeling shoot tip and performing specific induction on the shoot tip to generate embryogenic callus; 4) again inducing the embryogenic callus to form a seedling and performing propagation; and 5) performing virus detection on the tissue culture seedling obtained in the step 4), so as to obtain the lily detoxified seedling when the detection result is qualified. Through two times of embryogenic callus induction, the lily detoxifying method solves the problems that many detoxifying materials are needed, the detoxified seedling is bad in resistance and detoxifiying is not thorough. The method effectively overcomes the genetic depression problem caused by a relatively high lily plantation frequency, has the characteristics of high detoxifying efficiency, simple operation and low production equipment requirements, and is capable of satisfying demands on large-scale production.

Description

technical field [0001] The invention relates to the technical field of testing or treating seeds before planting, in particular to a method for detoxification of lily seedlings; specifically, a method for detoxification of lily based on the specific induction of embryogenic callus. Background technique [0002] With the expansion of lily production and bulb trade in the world, the diseases and insect pests of lily have spread rapidly, seriously affecting the yield and quality of lily. Among them, viral disease is one of the major worldwide diseases that have occurred recently. With the rapid increase in the number of lily imports, introductions, and planting areas in my country, as well as irregular planting and self-propagation of bulbs, viral diseases have become prevalent in various lily planting areas in my country, with a general incidence rate of 40%-50%. The poisonous rate of the bulbs is over 90%. Not only has it seriously affected the yield and quality of lily, but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 孙明伟赵统利邵小斌朱朋波汤雪燕刘兴满明军陈翠竹
Owner 龙山县喜乐百合食品有限公司
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