Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid

A receptor agonist, detection method technology, applied in material analysis by observing the effect on chemical indicators, color/spectral property measurement, analysis by chemical reaction of materials, etc. The scope and speed of screening, the high false positive rate of products, and the impact of rapid circulation of livestock and poultry products have achieved the effect of online detection, simple and fast operation process, and improved detection efficiency.

Active Publication Date: 2015-01-21
CHINA AGRI UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the screening methods for rapid detection of β-receptor agonist illicit drugs on the market are mainly enzyme-linked immunosorbent assay kits and colloidal gold test strips. There are only a few rapid detection kits or test strips for β-agonist drugs such as clenbuterol, ractopamine, and albuterol on the market. Moreover, the false positive rate of domestic related products is high, which greatly limits the scope and speed of rapid screening by the government and enterprises, and has a great impact on the rapid circulation of livestock and poultry products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid
  • Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid
  • Detection method and kit for detecting beta-receptor stimulant drugs in animal body fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 is used to illustrate the effect when the method provided by the present invention is used to detect animal body fluids containing different concentrations of β-receptor agonist drugs.

[0063] Preparation of samples with different concentrations to be tested: Add clenbuterol to 1 mL of pig serum that does not contain β-receptor agonist drugs, and prepare concentrations of 1.6, 1.2, 0.8, 0.4, 0.2, 0.1, 0.02, 0.01, 0μg / mL samples to be tested.

[0064] Take 300 μL of the pig serum to be tested at the above concentrations in a 1.5 mL centrifuge tube, and do two replicates for each sample; add 600 μL of absolute ethanol, vortex for 1 min, and let stand for 5 min;

[0065] Centrifuge the centrifuge tube at 12,000 rpm for 10 min at 4°C to obtain the supernatant;

[0066] In a 1.5mL centrifuge tube, add 80μL concentration of 0.4×10 -3 MHAuCl 4 solution, 80 μL of CTAC solution with a concentration of 60 mM, 40 μL of the above supernatant, and 600 μL of PBS solutio...

Embodiment 2

[0072] The animal body fluid samples were tested in the same way as in Example 1, the difference was that the samples of different concentrations to be tested were prepared in the following way: adding Clent to pig urine that did not contain β-receptor agonist drugs Luo, respectively configured as samples to be tested with concentrations of 1.6, 1.2, 0.8, 0.4, 0.2, 0.1, 0.02, 0.01, and 0 μg / mL. Observe the color change of the generated gold nanoparticles, see the test results figure 2 . Use TU-1901UV-vis type spectrophotometer to the absorbance value of each sample at 520nm wavelength, the results are listed in Table 1.

[0073] from figure 2 It can be seen from the results that with the increase of the concentration of clenbuterol in pig urine, the color of the reaction mixture tends to gradually deepen.

Embodiment 3

[0075] The animal body fluid samples were tested in the same manner as in Example 1, except that the samples of different concentrations to be tested were prepared by adding ractopamine to 1 mL of bovine emulsion that did not contain β-receptor agonist drugs, Prepare samples to be tested with concentrations of 1.6, 1.2, 0.8, 0.4, 0.2, 0.1, 0.02, 0.01, and 0 μg / mL, respectively. Observe the color change of the generated gold nanoparticles, see the test results image 3 . Use TU-1901UV-vis type spectrophotometer to the absorbance value of each sample at 520nm wavelength, the results are listed in Table 1.

[0076] from image 3 As can be seen from the results, with the increase of ractopamine in the milk emulsion, the color of the reaction mixture is gradually deepened from light red to red.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Outer diameteraaaaaaaaaa
Lengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a detection method for detecting beta-receptor stimulant drugs in animal body fluid. The method comprises the steps of enabling animal body fluid to be detected to come into contact with acetonitrile, ethyl alcohol or a multiwalled carbon nanotube for the first time to obtain sample extraction liquid, enabling the sample extraction liquid to come into contact with chloroauric acid solution and cetyl trimethyl ammonium bromide solution in PBS (Phosphate Buffer Solution) for the first time to obtain a reaction mixture, incubating the reaction mixture for 4-12min at 60-100 DEG C and observing the color change of the reaction mixture. The invention also provides a detection kit for detecting beta-receptor stimulant drugs in animal body fluid. The method can conveniently and rapidly detect the beta-receptor stimulant drugs in animal body fluid.

Description

technical field [0001] The invention relates to a rapid detection method and a detection kit for biological samples, in particular to a detection method and a kit for beta-receptor agonist drugs in animal body fluids. Background technique [0002] Residues of veterinary drugs, additives and harmful compounds are important factors affecting the safety of animal products in our country. The illegal use of β-receptor agonist drugs (commonly known as "clenbuterol") in the breeding process at home and abroad has caused many food safety incidents, resulting in solved the problem of food safety in the world. It not only threatens human health, but also has teratogenic, mutagenic and carcinogenic effects; at the same time, it seriously affects the export trade of livestock and poultry products, causing huge economic losses. The Chinese government has banned the use of "clenbuterol" in animal breeding since 1997. After years of treatment, the results have been remarkable. However, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78G01N21/31
Inventor 贺平丽王宪李溱李婷婷
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com