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A strain of Lactobacillus jansnii and its application

A technology of Lactobacillus johnsonii and bacterial agent, applied in the field of microorganisms, can solve problems such as easy recurrence, and achieve the effect of strong adhesion

Active Publication Date: 2017-01-18
北京益生苑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Urogenital tract infection is a common and frequently-occurring disease in women, and is prone to recurrence. Although antibiotics have a good short-term effect, there is a 40% recurrence rate

Method used

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  • A strain of Lactobacillus jansnii and its application
  • A strain of Lactobacillus jansnii and its application
  • A strain of Lactobacillus jansnii and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, isolation and identification of Lactobacillus jansnii strain

[0027] 1. Isolation of Lactobacillus jansii strains

[0028] The vaginal secretion samples collected from more than 200 cases of healthy women without symptoms and signs of vaginitis were numbered sequentially, inoculated into MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Hundreds of isolated bacteria were tested for bacterial species identification, hydrogen peroxide production and adhesion to vaginal epithelial cells, and one of them was named Lactobacillus jannis with strong adhesion and hydrogen peroxide production for IMJ-19.

[0029] 2. Identification of Lactobacillus jansii strains

[0030] (1) Morphological identification

[0031] Inoculate the bacterial strain IMJ-19 isolated in the above step 1 on the MRS...

Embodiment 2

[0043] Example 2, Functional analysis of Lactobacillus jansnii IMJ-19

[0044] 1. Lactobacillus jansii IMJ-19 produces H 2 o 2 detection

[0045] (1) H 2 o 2 The making of the standard curve: first use the aqueous solution of piperazine-N, N'-di-ethanesulfonic acid (Piperazine-N, N-Bis 2-ethanesulfonic acid, PIPES) with the concentration of 100mmol / L to make the volume percentage content 30% %H 2 o 2 Aqueous solution (equivalent to 9.128mol / L) stock solution is diluted to 1mol / L, and then the above 1mol / L H with 100mmol / L PIPES aqueous solution 2 o 2 Dilute to 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L respectively, and record the above dilutions and 100mmol / L PIPES aqueous solution as H 2 o 2 working solution; then take 100 μL of the above H 2 o 2 The working solution was mixed with 100 μL of 20 mmol / L TMB (tetramethylbenzidine) aqueous solution and 2 μL of 1 mg / mL horseradish peroxidase (purchased from TaKaRa Company), incubated at 16 °C for 10 min, and mea...

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Abstract

The invention discloses a strain of Lactobacillus jansii and application thereof. Lactobacillus jensenii (Lactobacillus.jensenii) disclosed by the present invention is named as IMJ‑19, and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on July 14, 2014, and its preservation number is CGMCC No.9439 . Lactobacillus jensenii (Lactobacillus.jensenii) IMJ‑19 disclosed by the invention has a strong ability to produce H2O2, can effectively inhibit the growth of foreign pathogenic bacteria and other conditional pathogenic bacteria, and has a strong effect on the vaginal epithelium. The adhesion of the cells ensures the colonization of the strain on the vaginal epithelium, and Lactobacillus jensii IMJ‑19 has a certain application prospect in the prevention and treatment of urogenital tract infections.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Lactobacillus jansii and its application. Background technique [0002] Urogenital tract infection is the most common but not paid attention to frequently-occurring disease of women, and the incidence rate is second only to respiratory tract infection, which can be as high as 40%. Women's physical and mental health will not only affect the happiness and stability of many families, but also affect the population quality and economic development of the whole society. The common pathogens of female urinary tract infection are Gram-negative bacilli, among which Escherichia coli is the most common, followed by Staphylococcus; most of these are normal flora on the human intestinal tract and skin and mucous membranes. Bacteria can migrate from the intestinal tract, skin and mucous membranes to the urinary system and reproduce in large numbers, causing urinary tract infections....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/00C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 朱宝利张瑞芬李晶王江
Owner 北京益生苑生物科技有限公司
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