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Protein composite scaffold capable of self-producing oxygen, and preparation method thereof

A technology of composite scaffolds and self-produced oxygen, applied in the field of protein composite scaffolds, can solve the problems of high cost, complicated operation, unsuitable for large-area tissue material modification, etc., and achieve the effect of improving the success rate

Active Publication Date: 2015-01-21
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of method is relatively complicated to operate, and the cost is high, and it is not suitable for the modification of large-scale tissue materials, so there is still a long way to go before clinical application.

Method used

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  • Protein composite scaffold capable of self-producing oxygen, and preparation method thereof
  • Protein composite scaffold capable of self-producing oxygen, and preparation method thereof
  • Protein composite scaffold capable of self-producing oxygen, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Obtain undyed normal black hair from a barber shop, wash it with regular shampoo for 1-2 times, and place it in a normal room temperature for drying.

[0034] 2. Soak the dried hair in the mixed solution of ethyl acetate and methanol for 24 hours, wherein the volume ratio of ethyl acetate and methanol is 3:1.

[0035] 3. After the hair is dried again, cut it into pieces and add it to the extract solution at a ratio of 1.5g / 10ml (dissolve 480.4g urea, 57.676g sodium dodecyl sulfate SDS, 95.045g Na 2 S 2 o 5 ), heated in an oil bath at 100°C for 30 minutes after immersion.

[0036] 4. Vacuum suction filtration, put the black extract in a dialysis bag, and dialyze for 5 days. The aqueous solution around the dialysis bag contains 0.1wt% Na 2 S 2 o 5 .

[0037]5. After the dialysis is completed, put the dialysis belt in 20wt% PEG (molecular weight 10000) aqueous solution, concentrate for 6 hours, adjust the concentration of reduced human hair keratin to 0.1g / mL, and...

Embodiment 2

[0044] 1. Obtain undyed normal black hair from a barber shop, wash it with regular shampoo for 1-2 times, and place it in a normal room temperature for drying.

[0045] 2. Soak the dried hair in the mixed solution of ethyl acetate and methanol for 24 hours, wherein the volume ratio of ethyl acetate and methanol is 3:1.

[0046] 3. After the hair is dried again, cut it into pieces and add it to the extract solution at a ratio of 1.5g / 10ml (dissolve 480.4g urea, 57.676g sodium dodecyl sulfate SDS, 95.045g Na 2 S 2 o 5 ), heated in an oil bath at 100°C for 30 minutes after immersion.

[0047] 4. Vacuum suction filtration, put the black extract in a dialysis bag, and dialyze for 5 days. The aqueous solution around the dialysis bag contains 0.1wt% Na 2 S 2 o 5 .

[0048] 5. After the dialysis is completed, put the dialysis belt in 20wt% PEG (molecular weight 10000) aqueous solution, concentrate for 6 hours, adjust the concentration of reduced human hair keratin to 0.1g / mL, an...

Embodiment 3

[0061] 1. Obtain undyed normal black hair from a barber shop, wash it with regular shampoo for 1-2 times, and place it in a normal room temperature for drying.

[0062] 2. Soak the dried hair in the mixed solution of ethyl acetate and methanol for 24 hours, wherein the volume ratio of ethyl acetate and methanol is 3:1.

[0063] 3. After the hair is dried again, cut it into pieces and add it to the extract solution at a ratio of 1.5g / 10ml (dissolve 480.4g urea, 57.676g sodium dodecyl sulfate SDS, 95.045g Na 2 S 2 o 5 ), heated in an oil bath at 100°C for 30 minutes after immersion.

[0064] 4. Vacuum suction filtration, put the black extract in a dialysis bag, and dialyze for 5 days. The aqueous solution around the dialysis bag contains 0.1wt% Na 2 S 2 o 5 .

[0065] 5. After the dialysis is completed, put the dialysis belt in 20wt% PEG (molecular weight 10000) aqueous solution, concentrate for 6 hours, adjust the concentration of reduced human hair keratin to 0.1g / mL, an...

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Abstract

The invention provides a protein composite scaffold capable of self-producing oxygen. The protein composite scaffold capable of self-producing oxygen comprises bioprotein, gelatin and an oxygen self-producing material. A mass ratio of the gelatin to the bioprotein is (0.5-5):10 and a mass ratio of the oxygen self-producing material to the bioprotein is (1-50):10. The protein composite scaffold capable of self-producing oxygen is compounded from the bioprotein, the gelatin and the oxygen self-producing material, and has physical strength superior to a membrane structure material constructed by using a pure protein combined solution. Meanwhile, the gelatin has a certain coating effect on the oxygen self-producing material, so that the provided protein composite scaffold can release oxygen at a constant speed and a high level; seed cells compounded on the protein composite scaffold can survive for a long time and microvessel ingrowth after the material is implanted is promoted. The protein composite scaffold is suitable for requirements of actual surgery operations, and finally increases success rate of urethral repair and reconstruction.

Description

technical field [0001] The invention relates to a protein composite scaffold, in particular to a protein composite scaffold capable of self-producing oxygen that can be used for urethral repair and reconstruction and a preparation method thereof. Background technique [0002] Complicated urinary tract diseases caused by various reasons have always been a difficult problem in the clinical treatment of urology. Although a variety of autologous tissues have achieved ideal results in urethral repair and reconstruction as substitute materials, in comparison, tissue engineering technology does not have the inherent defect of "sacrificing normal tissue at the expense of surgical trauma to repair tissue defects", so it is more feasible. As the main direction of future urethral reconstruction development. In the existing domestic and foreign literature reports, the research on various tissue engineering materials or material-seed cell complexes in urethral repair and reconstruction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/02
Inventor 冯超李喆徐月敏吕向国
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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