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Carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof

A carcinoembryonic antigen and kit technology, which is applied in measuring devices, instruments, disease diagnosis, etc., can solve the problem of high detection cost

Inactive Publication Date: 2015-01-14
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current carcinoembryonic antigen (CEA) colloidal gold test strip is convenient, compared with the dot gold standard immunoassay, its preparation process requires enzymes, which makes the detection cost relatively high

Method used

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  • Carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof
  • Carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof
  • Carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof

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Experimental program
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Embodiment Construction

[0040] (1) The preparation method of a bottle of CEA standard solution with a volume of 10 milliliters at a concentration of 100 ng / ml. Take a reagent bottle with a volume of 20 ml, add 1 ml of CEA standard solution with a concentration of 1 μg / ml into it with a pipette gun, and then add 10 ml of double distilled water to make a concentration of 100 ng / ml and a volume of 10 ml CEA standard solution.

[0041] (2) The preparation method of 5% BSA solution. Weigh 5 g of BSA into a 100 ml volumetric flask with a microbalance, dissolve it with 100 ml of double distilled water and adjust the volume to 100 ml.

[0042] (3) Preparation method of colloidal gold-labeled anti-CEA monoclonal antibody. First make nano gold, and then use nano gold to record anti-CEA monoclonal antibody. The specific production method is:

[0043] 3.1 The method of making colloidal gold: Take 49.5 milliliters of double-distilled deionized water, add 0.5 milliliters of chloroauric acid solution with a con...

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Abstract

The aim of the invention is to provide a carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof, the carcino-embryonic antigen rapid simple detection kit is lower in cost than a test strip; by use of specific responses of human carcinoembryonic antigen and anti carcinoembryonic antigen antibody, the carcinoembryonic antigen and the antibody are bonded onto a NC (nitrocellulose) membrane and colloidal gold particles, a colloidal gold and antibody complex is added onto the NC membrane, and whether the CEA is negative or positive is determined by observation of the color. When the CEA content is lower than 2.5uM, a 2.5uM CEA standard product is not significantly different from a 0uM standard product in color development, so when the CEA content is lower than 2.5uM, the CEA is determined to be negative; when the CEA content is in the 2.5-10uM range, the detection point color is significantly shallower than the 0uM spot color, the CEA is determined to be weak positive; when the CEA content is more than 10uM, and no red spot appears, the CEA is determined to be positive.

Description

technical field [0001] The invention relates to a method for fast and simple detection of carcinoembryonic antigen (CEA), in particular to the production of anti-CEA antibody immunogold and its detection kit. Background technique [0002] Carcinoembryonic antigen (CEA) is a protein with a relative molecular mass of 1.8×10 3 The glycoprotein molecule is named for its normal secretion in the embryo. Abnormal expression of CEA can be detected in the serum of patients with stomach and intestinal tract at first, and then gradually detected in the serum of patients with lung cancer, liver cancer, breast cancer and pancreatic cancer. Early prevention of carcinogenesis, lung cancer, liver cancer, breast cancer and pancreatic cancer is of great significance. Currently, the most common detection methods are chemiluminescence and ELISA. Because these methods require certain instruments and equipment, and the detection time is long, they are suitable for the detection of a large numb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531G01N33/574
CPCG01N33/57473G01N33/531G01N2800/7028
Inventor 曾红娟李宗程远
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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