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Application of RhoA recombinant protein in preparation of culture solution for improving embryo freezing resistance and recovery rate after thawing

A technology for embryo culture medium and recombinant protein, which is applied in the field of preparing culture medium for improving the freezing resistance of embryos and the recovery rate after thawing, can solve the problems of reduced embryo survival rate, embryo toxicity, embryo damage, etc., to reduce adverse effects and enhance Low temperature resistance, damage reduction effect

Active Publication Date: 2015-01-14
BEIJING UNIV OF AGRI
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Problems solved by technology

However, 40% of the embryos will be damaged during the freezing process after freezing, which will reduce the survival rate of the embryos after transplantation, and these freezing liquids will still bring more or less toxicity and harm to the embryos

Method used

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  • Application of RhoA recombinant protein in preparation of culture solution for improving embryo freezing resistance and recovery rate after thawing
  • Application of RhoA recombinant protein in preparation of culture solution for improving embryo freezing resistance and recovery rate after thawing
  • Application of RhoA recombinant protein in preparation of culture solution for improving embryo freezing resistance and recovery rate after thawing

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Embodiment 1

[0023] 1. Materials

[0024] 1.1 Main instruments and equipment

[0025] Pipette: Beijing Baijing pipette (1mL, 200μL, 100μL, 10μL)

[0026] Medical centrifuge: Beijing Medical Centrifuge Factory, LDZ4-0.8

[0027] CO 2 Incubator: Heal Force, HF90

[0028] Stereo Microscope: Jiangnan Microscope Factory, X11-3

[0029] Ultra-clean bench: Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd., ZHJH-1112B

[0030] Electronic balance: Beijing Sartorius Instrument System Co., Ltd., BS124S

[0031] pH meter: Thermo, 868

[0032] Precision heating plate: Betta Instruments, MEH-2

[0033] Water bath: Beijing Changan Scientific Instrument Factory, HH·S1-Ni

[0034] 1.2 Main reagents

[0035] 1.2.1 In vitro recombinant human-derived RhoA protein was purchased from Sino Biological Company, catalog number: 12441-H07B.

[0036] 1.2.2 Reconstituted recombinant protein in vitro culture solution: Prepare 10 μg / μl stock solution with 50 μg of recombinant protein in vitro. S...

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Abstract

The invention discloses an application of RhoA recombinant protein in preparation of a culture solution for improving embryo freezing resistance and recovery rate after thawing. The research finds that by addition of in vitro recombined RhoA protein to a culture solution of normally hatched embryos of mice, compared with a control group, the thawing recovery rate of normally hatched embryos can be significantly increased. The RhoA recombinant protein is applied to the field of embryo freezing for the first time, through addition of the RhoA recombinant protein, the freezing resistance and the recovery rate after thawing of the embryos are significantly increased, and use of a toxic cryoprotectant is reduced to a certain extent, so that the adverse effects on the embryos are reduced, and the RhoA recombinant protein has a wide application prospect in the technical field of cryopreservation of the embryos.

Description

technical field [0001] The invention relates to a new application of the RhoA recombinant protein, in particular to the application of the RhoA recombinant protein in the preparation of a culture medium for improving the freezing resistance of embryos and the recovery rate after thawing. The invention belongs to the technical field of embryo cryopreservation. Background technique [0002] In the field of livestock breeding, embryo cryopreservation technology has developed rapidly. In animal husbandry, it has become a very common operation method to thaw the cryopreserved blastocysts and then transplant them into recipient female animals at an appropriate time. . So far, the use of imported polyethylene glycol as a freezing liquid in the actual production process has achieved good results in actual freezing applications. However, 40% of the embryos will be damaged during the freezing process after freezing, which reduces the survival rate of the embryos after transplantation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073A01N1/02
Inventor 郭勇倪和民刘云海顾美超齐晓龙
Owner BEIJING UNIV OF AGRI
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