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Ceriporia lacerata strain and application thereof

A technology for tearing cereus and strains, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problem of low yield and achieve the effects of sufficient substrate conversion, high yield, and high surface activity

Inactive Publication Date: 2015-01-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hua Zhaozhe and others studied Candida antarctic WHS112 using soybean oil as the sole carbon source to produce mannose erythritol lipids (Hua Zhaozhe, Chen Jian, Zhu Wenchang, et al. Candida antarctic WSH112) Research on the production of biosurfactants and the degradation of n-alkanes. Journal of Nanjing University (Natural Science), 1998, 32(2): 149-154), but the yield is not high

Method used

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  • Ceriporia lacerata strain and application thereof
  • Ceriporia lacerata strain and application thereof
  • Ceriporia lacerata strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation, purification, identification and preservation of strains

[0020] 1. Isolation and purification of soil strains

[0021] The dilution plate coating method was used to separate and screen from the soil to obtain a fungal strain. Specifically: take 10 g of soil, add 100 mL of sterile water, and mix well to make a suspension. Take 100 μL of the suspension on the PDA plate medium and spread it evenly. Place it in an incubator at 25-28°C for 7 days, until a colony visible to the naked eye grows. Pick out a single fluffy colony, inoculate it into a new medium, and perform purification and separation to obtain a single strain.

[0022] 2. Mutagenesis of strains

[0023] Using normal temperature plasma mutagenesis technology, mutagenesis of the above-mentioned single strains is used to screen and obtain the target strains. Specifically, the fungal strain was cultured on a PDA medium for 4 days, a loop was inoculated into a liquid medium containing glass beads, ...

Embodiment 2

[0028] Example 2 Preparation of mannose erythritol by fermentation of Ceriporia lacerate CHZJU

[0029] 1. Strain activation: use PDA medium as the activation medium, insert Ceriporia lacerate CHZJU, activate and culture at 28°C for 3 days, and wait until the mycelium is overgrown on the plate.

[0030] 2. Seed culture: Pick 3 pieces of 1cm×1cm hyphae from the plate medium and put them into a 250mL Erlenmeyer flask containing 50mL seed culture solution, and keep the cultured seeds at 28℃ and 220rpm for 4 days. Centrifuge the liquid, take the bacterial cells, and wash 3 times with 0.9% sodium chloride solution;

[0031] Among them, the seed culture solution contains glucose 4.0%, sodium nitrate 0.3%, magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.03%, yeast powder 0.1%, and distilled water.

[0032] 3. Fermentation culture: the bacterial cells were put into a 500mL Erlenmeyer flask containing 100mL fermentation medium at a wet weight ratio of 10g / L, and cultured at 28°C and ...

Embodiment 3

[0035] Example 3 Preparation of mannose erythritol by fermentation of Ceriporia lacerate CHZJU

[0036] 1. Strain activation: use PDA medium as the activation medium, insert Ceriporia lacerate CHZJU, activate and culture at 30°C for 3 days, and wait until the mycelium is overgrown on the plate.

[0037] 2. Seed culture: Pick 3 pieces of 1cm×1cm hyphae from the plate medium and put them into a 250mL Erlenmeyer flask containing 50mL seed culture solution. At 30℃, 220rpm conditions for 4 days; put the cultivated seeds Centrifuge the liquid, take the bacterial cells, and wash 3 times with 0.9% sodium chloride solution;

[0038] Among them, the seed culture solution contains glucose 4.0%, sodium nitrate 0.3%, magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.03%, yeast powder 0.1%, and distilled water.

[0039] 3. Fermentation culture: The bacterial cells were put into a 500mL Erlenmeyer flask containing 100mL fermentation medium at a wet weight ratio of 10g / L, and cultured at 30°C...

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Abstract

The invention discloses a Ceriporia lacerata strain which is named Ceriporia lacerata CHZJU. The Ceriporia lacerata stain is collected at China Center for Type Culture Collection, the collection date is May 28th, 2014, the collection number is CCTCC M2014230, and the address of the collection unit is Wuhan University, Wuhan, Hubei, China. The strain can be used for fermenting a vegetable oil substrate to produce mannosylerythrutol lipid; and the substrate is a renewable resource with wide sources, the substrate and product are non-toxic and environment-friendly, and the fermentation liquid has high surface activity. When the strain is used for fermentation to produce mannosylerythrutol lipid, the mycelial mass is small in the fermentation process, the substrate is converted sufficiently, and the crude product yield is high.

Description

Technical field [0001] The invention relates to a fungus, in particular to a Ceriporia lacerate strain and its application. Background technique [0002] Mannosylerythritol lipids (MELs) is a glycolipid biosurfactant. MELs not only have good surface activity, emulsification, biodegradability, low critical micelle concentration, but also have many special physiological activities, such as inhibiting the growth of microorganisms, inducing cell mutation, and being able to differentiate human myeloid leukemia cell lines and black cells. It can be used in environmental protection, food, cosmetics, medicine and other industries. It can improve the efficiency of gene transfection and have strong coordination ability with glycoprotein. [0003] There are few domestic reports on the biotransformation research of MELs. Hua Zhaozhe et al. studied Candida antarctic WHS112 using soybean oil as the sole carbon source to produce mannose erythritol esters (Hua Zhaozhe, Chen Jian, Zhu Wenchang, e...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/44C12R1/645
CPCC12P19/44C12N1/145C12R2001/645
Inventor 陈启和范琳琳董亚晨李宏吉焦志华
Owner ZHEJIANG UNIV
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