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A plant growth-promoting endophytic bacterium with polycyclic aromatic hydrocarbon degradation function and its application

A technology for polycyclic aromatic hydrocarbons and degrading bacteria, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of unseen patent research and application reports, and achieve the effects of ensuring the safety of agricultural products, low production costs, and promoting plant growth

Active Publication Date: 2017-01-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for persistent organic pollutants such as PAHs, there are still few data reports at home and abroad, and there are no relevant patent research and application reports

Method used

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  • A plant growth-promoting endophytic bacterium with polycyclic aromatic hydrocarbon degradation function and its application
  • A plant growth-promoting endophytic bacterium with polycyclic aromatic hydrocarbon degradation function and its application
  • A plant growth-promoting endophytic bacterium with polycyclic aromatic hydrocarbon degradation function and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacterial Strains

[0029] Disinfect the fresh A. argentina plants collected from the sewage outlet of Nanjing Jiangning Yangzi Petrochemical Aromatics Plant: rinse the plants with tap water, rinse them with 75% alcohol in an ultra-clean bench for 3-5 minutes, and then rinse them with sterile water for 3-5 minutes. 4 times, then rinse with 1% sodium hypochlorite solution for 2 to 5 minutes, and finally rinse with sterile water several times. Transfer the surface-sterilized plant samples to LB solid plates, and after incubating them at 30°C for 24 hours, check whether there is bacterial growth on the plates. If no colony grows, it means that the surface of the plant is disinfected successfully.

[0030] Move the tested and sterilized plants into a sterile mortar, cut them into pieces with sterilized scissors, add sterile water to grind them thoroughly, take the supernatant and add it to 100mL inorganic salt medium with 5% inocul...

Embodiment 2

[0037] Embodiment 2 Fermentation of restoration bacterial agent

[0038] The process of using the above-mentioned strain Pn2 to produce the repairing bacterial agent is as follows: slant seed—shake bottle seed liquid—seed tank—product (the packaging dosage form is liquid bacterial agent).

[0039] 1) Inoculate the test tube species of Massilia sp. Pn2 with the preservation number CGMCC NO.1.12927 in the fermentation medium, and shake and cultivate to the logarithmic phase;

[0040] 2) Inoculate the above-mentioned cultured strains into the seed tank with an inoculation amount of 5%, and cultivate to the logarithmic phase;

[0041] 3) The seed liquid is connected to the production tank for cultivation according to the inoculum amount of 10%;

[0042] 4) During the cultivation process of seed tanks and production tanks, the ventilation rate of sterile air is 1:1.2, the stirring speed is 200 rpm, the cultivation temperature is 30°C, and the cultivation time of the whole process ...

Embodiment 3

[0045] Example 3 Biodegradation experiment of PAHs by bacterial strain Pn2 in culture medium

[0046] In the inorganic salt culture medium (with embodiment 1), add final concentration and be 150mg L ‐1 The phenanthrene was inoculated into strain Pn2 (CGMCC NO.1.12927) with a 5% inoculum amount, and inoculated with inactivated strain Pn2 as a control, and cultured in a constant temperature shaker at 30°C. Take regular samples to detect the strain Pn2 at 150 mg·L ‐1 The growth of phenanthrene as the only carbon source and the degradation of phenanthrene, the results are shown in figure 2 . Strain Pn2 can grow well with phenanthrene as the sole carbon source, and within 3 days to an initial concentration of 150mg·L ‐1 The degradation rate of phenanthrene reaches more than 95%.

[0047] According to the above method, the bacterial strain Pn2 (CGMCC NO.1.12927) was sequentially inserted into the naphthalene-containing (100mg L ‐1 ), acenaphthyl (100mg·L ‐1 ), anthracene (50m...

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Abstract

The invention discloses a plant growth-promoting endophytic bacterium with polycyclic aromatic hydrocarbon degradation function and application thereof. A plant growth-promoting endophytic bacterium Massilia sp. Pn2 with the function of degrading polycyclic aromatic hydrocarbons was preserved in the General Microbiology Center of China Microbiological Cultures Preservation Management Committee on August 26, 2014, and the strain preservation number is CGMCC 1.12927. The microbial agent prepared by using this strain can degrade more than 95% of phenanthrene with an initial concentration of 150mg L‐1 within 3 days under laboratory shake flask culture conditions. and pyrene. Using the root soaking treatment to colonize the plant root surface with the bacterial agent and then plant the plants in the phenanthrene-contaminated nutrient solution can reduce the phenanthrene content in the plant by more than 50% in a short period of time, significantly reducing the phenanthrene pollution of the plant risk, and has obvious promoting effect on plant growth.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental polycyclic aromatic hydrocarbons (PAHs) pollution, and relates to a plant growth-promoting endophytic bacterium with the function of degrading PAHs, a bacterial agent for its production, and an application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are an important class of persistent organic pollutants commonly found in the soil environment composed of two or more benzene rings, which are highly hydrophobic and difficult to degrade. As hydrophobic organic pollutants, PAHs in soil can be absorbed by plants and accumulate in the body. How to reduce the risk of plant PAHs pollution is one of the current research hotspots in the field of agricultural environment. [0003] Plant endophytic bacteria refer to a type of microorganisms that can colonize in the gaps or cells of healthy plant tissues and establish a harmonious symbiotic relationship with ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01
CPCA62D3/02A62D2101/20B09C1/10C02F3/34C02F2101/327C12N1/205C12R2001/01
Inventor 高彦征刘娟刘爽孙凯
Owner NANJING AGRICULTURAL UNIVERSITY
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