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Method for extracting grifola frondosa fermentation broth polysaccharide by virtue of dual-membrane process

A technology of Grifola frondosa and fermentation liquid, applied in the field of microorganisms, can solve the problems of production cost and product quality, alcohol and labor consumption, low monosaccharide removal rate, etc., and achieve reduced steam consumption, low supporting cost, and high filtration accuracy high effect

Inactive Publication Date: 2015-01-07
TIANJIN SHENGJI GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the alcohol precipitation process, since monosaccharides are often accompanied by polysaccharides, the removal rate of monosaccharides is low. Therefore, multiple alcohol precipitations are required, which consumes more alcohol and labor, and the production cost is higher; at the same time, the traditional heat Concentration method consumes a lot of steam, which has a great impact on production cost and product quality

Method used

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Embodiment 1

[0023] The method for extracting polysaccharides from Grifola frondosa fermented liquid of the present invention is carried out according to the following steps:

[0024] 1. Source of Grifola frondosa fermentation broth: The Grifola frondosa strain is provided by the China Microbiological Culture Collection and Management Center, and the strain is transferred to a slant medium for conventional culture at 26-28 degrees for about 10 days to obtain an activated Grifola frondosa strain; The activated Grifola frondosa strain was inoculated into the seed medium, and cultured with shaking at 26-28 degrees for 4-5 days to obtain the primary seed liquid of Grifola frondosa; the primary seed liquid was inserted into the secondary seed medium, and cultivated at 26-28 degrees About 4 days; the secondary seed medium is inserted into the fermentation medium, cultivated at 26-28 degrees for about 10 days, and the fermentation is completed to become the Grifola frondosa fermentation liquid.

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Abstract

The invention discloses a method for extracting grifola frondosa fermentation broth polysaccharide by virtue of a dual-membrane process. According to the method for extracting the grifola frondosa fermentation broth polysaccharide by virtue of the dual-membrane process, the grifola frondosa fermentation broth polysaccharide is obtained by processing and separating grifola frondosa fermentation broth through a micro-filtration membrane and an ultra-filtration membrane under a specific condition. Due to adoption of the method, the traditional 'water-extraction and ethanol-precipitation' extraction mode of grifola frondosa fermentation broth polysaccharide is changed, the purification effect of grifola frondosa fermentation broth polysaccharide is improved, the amount of ethanol in the production is reduced and the economic efficiency is increased; and meanwhile, by adopting the method, the emission of filtrate wastewater can be reduced and social benefits are significantly increased.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for extracting fungal exopolysaccharides, more specifically, a new method for extracting polysaccharides from Grifola frondosa fermentation broth based on membrane technology. Background technique [0002] Grifola frondosa fermentation broth polysaccharides are polysaccharides composed of D-glucose, D-xylose, D-fucose, D-mannose and other monosaccharides. The extracted and refined proteoglycan substance with physiological activity is the main active ingredient of Grifola frondosa. Experiments have shown that the polysaccharides in fermented liquid of Grifola frondosa have inhibitory effects on various viruses, such as HIV (HIV.1), herpes simplex virus, cytomegalovirus, influenza virus and so on. Grifola frondosa fermented polysaccharides can improve the immune function of the human body, increase white blood cells, and the polysaccharides fermented from Grifola frondosa ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王连民刘珂飞高应瑞
Owner TIANJIN SHENGJI GRP CO LTD
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