Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid propagation and cultivation method for tissue culture seedlings of calotropis gigantea, white-flower calotropis gigantea and large calotropis gigantea

The technology of a white-flowered oxhorn melon and its cultivation method is applied in horticultural methods, botanical equipment and methods, horticulture, etc. It can solve the problems of limited number of seedlings, long time for seedlings, difficulty in meeting the needs of artificial large-scale planting of seedlings, etc., and achieves The effect of multiple economic benefit services

Inactive Publication Date: 2015-01-07
黄惠民
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for rapid propagation of tissue culture seedlings of ox horn melon, white flower ox horn melon, and large ox horn melon, which mainly solves the problem of large-scale planting of wild ox horn melon plants, which requires a long time from seed sowing to seedling growth. The quantity is limited, and it is difficult to meet the technical problems of artificial large-scale planting of seedlings. It adopts the plant tissue culture or in vitro culture of the horned melon, and separates the various tissues or organs and cells that meet the needs from the horned melon plant, protoplasts, The cambium, parenchyma, mesophyll tissue, endosperm, etc. are cultured by aseptically producing callus from various organs, and the callus is then redifferentiated to form a regenerated plant of croissant melon

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In 2009, in the tissue culture seedling workshop of the nursery base in Kunming, Yunnan, in the test of the horn melon tissue culture seedlings, the stem side buds were used to obtain it, and the first step was taken: the material of the horn melon explant; the second step: the preparation of the medium; the third step: Sterilization of the explant material of the horned melon; Step 4: sterile inoculation; Step 5: medium cultivation; Step 6: hardening, domestication and transplanting. Obtain 210 tissue culture seedling plants, carry out nursery nursery success.

[0056] The new plants were transplanted out of the nursery as seedlings, and 191 tissue-cultured seedlings survived. They bloomed and bear fruit after 10 months of growth, and the survival rate was 94%.

Embodiment 2

[0058] In 2010, at the Planting Research Institute of Dongchuan District, Kunming City, Yunnan, borrowed its equipment to carry out the experiment on the tissue culture seedlings of the horned melon, which was obtained by using the horned melon receptacle, through the first step: the extraction of the horned melon explants; the second step: the preparation of the medium; the steps Step 3: Sterilization of the explant material of the horned melon; Step 4: Aseptic inoculation; Step 5: Culture medium; Step 6: Seedling hardening, acclimatization and transplanting. Obtain 260 tissue culture seedling plants, carry out tending success in vegetable field. 247 plants survived, flowering in 7 months and bearing fruit in 9 months, the survival rate is over 95%.

Embodiment 3

[0060]In 2012, in the seedling farm of Yulin Forest Farm, Guangxi, the tissue culture seedlings of the horned melon were obtained by using the new leaves of the horned melon to germinate, through the first step: the selection of the explant of the horned melon; the second step: the preparation of the medium; the third step: the outer part of the horned melon The implant material is sterilized; Step 4: sterile inoculation; Step 5: Medium cultivation; Step 6: Seedling hardening and transplanting. Obtained 420 tissue cultured seedling plants, and carried out successful nursery cultivation. Transplanted on the hillside next to the eucalyptus forest, 371 plants survived, bloomed in 6 months and borne fruit in 8 months, and the survival rate was about 83%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid propagation and cultivation method for tissue culture seedlings of calotropis gigantea, white-flower calotropis gigantea and large calotropis gigantea. The method comprises the following steps: (1) selecting an explant of the calotropis gigantea; (2) preparing culture mediums; (3) sterilizing the explant materials of the calotropis gigantea; (4) performing bacteria-free inoculation; (5) culturing the culture mediums; (6) exercising, domesticating and transplanting seedlings. The method disclosed by the invention mainly solves the technical problems that in the scale cultivation of the wild calotropis gigantea, the time that seeds grow into seedlings is longer, the number of cultured seedlings is limited, and the requirement of planting seedlings in a large scale manually is difficult to meet. The calotropis gigantea plant tissue culture or in-vitro culture is adopted, all parts of tissue or organs, cells, protoplasts, cambiums, parenchyma, mesophyll tissue, endosperm and the like, which can meet demands, are separated from the plant bodies of the calotropis gigantea, through bacteria-free operation, the callus tissue produced on each organ is cultured, and through the redifferentiation of the callus tissue, calotropis gigantea regenerating plants are formed.

Description

technical field [0001] The invention relates to a method for growing seedlings of plants, in particular to a method for rapid propagation of tissue-cultured seedlings of oxhorn melon, white-flowered oxhorn melon and large oxhorn melon. Background technique [0002] Family name: Astragalus. [0003] Genus name: Crow's horn melon. [0004] Chinese species name: ox horn melon, white flower ox horn melon, big ox horn melon. [0005] Latin scientific name: Apocynaceae; nickname: Calotropis R. Br. [0006] The croissant melon is an upright shrub, perennial up to 3m-4m, with flowers and fruits in the same period for about 11 months throughout the year. [0007] The oxhorn melon, the white-flowered oxhorn melon, and the big oxhorn melon in the various countries mentioned above are wild plants, and the large-scale planting of the oxhorn melon in the agriculture and forestry planting industry is blank. [0008] Since 2005, the inventors have discovered the wild species of horned m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 黄惠民
Owner 黄惠民
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com