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Test paper strip for quick detection of tolclofos-methyl by colloidal gold and preparation method of test paper strip

A technology for detecting test paper and phoxim gold, which is applied in the field of rapid detection test strips of phoxim colloidal gold and its preparation, can solve problems such as difficulty in satisfying fast, on-line detection, application limitations of ELISA method, and unintuitive detection results, etc. To achieve the effect of facilitating promotion, displaying the image of the results, and judging the image

Inactive Publication Date: 2014-12-24
JURONG AGRI TECH PROMOTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods include high performance liquid chromatography (High performance liquid chromatography, HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), liquid-mass tandem method (HPLC-MS) Although these instrumental analysis methods can achieve high detection sensitivity, they require complex and expensive instruments and professional operators. In addition, the sample pretreatment is cumbersome and time-consuming, and the detection cost is high. The need for fast, online testing
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA method is greatly limited.

Method used

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  • Test paper strip for quick detection of tolclofos-methyl by colloidal gold and preparation method of test paper strip
  • Test paper strip for quick detection of tolclofos-methyl by colloidal gold and preparation method of test paper strip
  • Test paper strip for quick detection of tolclofos-methyl by colloidal gold and preparation method of test paper strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A phoxim colloidal gold rapid detection test strip, the test strip contains a liner 1 and a sample pad 2, a gold standard binding pad 3, a cellulose film 4 and a water-absorbent pad 7 arranged on the liner in sequence, the The liner 1 is a tough material that does not absorb water, and the tough material is made of a hard plastic strip. The gold label binding pad 3 is adsorbed with a phoxim gold label antibody, and the cellulose film 4 has an invisible Detection line 5 and invisible control line 6, the invisible detection line 5 is printed with carrier protein solution coupled with phoxim hapten, and the invisible control line 6 is printed with rabbit anti-mouse IgG antibody. The water-absorbing pad 7 is water-absorbing filter paper or oil filter paper with strong water-absorbing capacity. Phoxim gold-labeled antibody is a colloidal gold-labeled phoxim monoclonal antibody. The carrier protein coupled with the phoxim hapten is bovine serum albumin (BSA). A protective f...

Embodiment 2

[0037] It is basically the same as Example 1, except that the tough material is made of non-absorbent hard paper strips, and the water-absorbing pad 7 is oil filter paper with strong water-absorbing capacity. The carrier protein coupled with phoxim hapten is chicken ovalbumin OVA. The sample pad 2 is made of nylon film, polyvinylidene fluoride film and polyester film. Cellulose membranes are pure cellulose membranes.

Embodiment 3

[0039] It is basically the same as in Example 1, except that the tough material is made of hard plastic strips, and the water-absorbing pad 7 is water-absorbing filter paper with strong water-absorbing capacity. The carrier protein coupled with phoxim hapten is human serum albumin HAS. The sample pad 2 is made of polyvinylidene fluoride film and polyester film. The cellulose film is a carboxylated cellulose film.

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PUM

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Abstract

The invention discloses a test paper strip for quick detection of tolclofos-methyl by colloidal gold. The test paper strip comprises a liner plate as well as a sample pad, a gold-labeled conjugate pad, a cellulose membrane and a water absorption pad which are arranged on the liner plate in sequence, wherein a tolclofos-methyl gold-labeled antibody is adsorbed in the gold-labeled conjugate pad; an invisible detection line and an invisible control line are arranged on the cellulose membrane; the invisible detection line is printed by a tolclofos-methyl hapten-coupled carrier protein solution; the invisible control line is printed by a rabbit antimouse IgG antibody. The test paper strip has the advantages of good specificity, high sensitivity, high simplicity, quickness, vividness, intuitiveness and accuracy for result display, and the like.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue immune analysis, and in particular relates to a phoxim colloidal gold rapid detection test strip and a preparation method thereof. Background technique [0002] Phoxim (Tolclofos-methyl) is a broad-spectrum organophosphorus insecticide used to control pests in stored grains and various foliage crops, and is also used to control mosquito adults, flies, and aquatic larvae and sanitary pests. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, some creatures that were not the intended target were also poisoned, including humans, fish and other creatures. [0003] At present, the detection methods for pesticide residues are mainly instrumental analysis methods and enzyme-linked immunoassay methods (ELISA). Instrum...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531
CPCG01N33/558G01N33/566G01N33/577
Inventor 韩志成
Owner JURONG AGRI TECH PROMOTION CENT
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