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Composition containing moringa oleifera leave water soluble extract as effective component and used for treating or preventing cancer and preparation method for moringa oleifera leave extract

A technology for water-soluble extracts and active ingredients, which is applied in the field of preparation of compositions for treating or preventing cancer containing the water-soluble extract of Moringa oleifera as an active ingredient and the Moringa oleifera extract, which can solve the influence of cancer cells. , inconvenience, decreased efficacy of anticancer drugs, etc.

Active Publication Date: 2014-12-24
KOREA ATOMIC ENERGY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Substances that have been used as anticancer agents in the past have shown toxicity not only to cancer cells but also to normal cells, causing many side effects
In addition, most of the existing anticancer agents are mainly water-insoluble substances, so they have the disadvantage that the absorption of anticancer substances in cells is difficult.
In order to dissolve these anticancer substances, many additional methods are used, so the side effects caused by them may also be caused, and the efficacy of anticancer agents may also be reduced. In order to increase absorption, absorption promoters are also used at the same time.
Moreover, conventional injection-type anticancer agents exhibit various toxicity and side effects, and inconvenience patients due to the difficulty of repeated injections.

Method used

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  • Composition containing moringa oleifera leave water soluble extract as effective component and used for treating or preventing cancer and preparation method for moringa oleifera leave extract
  • Composition containing moringa oleifera leave water soluble extract as effective component and used for treating or preventing cancer and preparation method for moringa oleifera leave extract
  • Composition containing moringa oleifera leave water soluble extract as effective component and used for treating or preventing cancer and preparation method for moringa oleifera leave extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Preparation of Moringa Leaf Extract according to Extraction Time

[0075] Dried Moringa leaves (M. oleifera leaves) were purchased from GL Network Co. Ltd. (cultivated in Chiang Mai, Thailand). 150 mg of dried Moringa oleifera leaves were immersed in 1 ml of water at 4° C., vortexing was performed for 30 seconds, and then stored with stirring in a refrigerator. The storage time of each embodiment in the cold room is different, that is, the soaking time of each embodiment is set differently as shown in Table 1 below. Each dip with different dip times was vortexed again for 1 min at room temperature. Thereafter, the insoluble portion of the floating substance was removed by centrifugation (12000 rpm, 10 minutes) 3 times, and then the supernatant (supern atant) was filtered (0.2 μM membrane filter) to obtain the final product. The final Moringa leaf extract was freeze-dried and stored at -20°C.

[0076] Table 1

[0077] Example 1 Example 2 Exa...

Embodiment 2

[0078] Example 2: Preparation of Moringa oleifera leaf extract according to extraction temperature

[0079] The Moringa oleifera leaf extract was prepared in the same manner as in Example 1, except that the soaking time was fixed at 30 minutes, and the temperature of each extraction solvent (water) was set differently as shown in the table below.

[0080] Table 2

[0081] Example 6 Example 7 Example 8 Example 9 20℃ 40℃ 60℃ 80℃

[0082] The freeze-dried Moringa oleifera leaf extract used in the following experiments was suspended in distilled water (D.W.) after the protein concentration was quantified so that the protein concentration was 20 mg / ml. The concentration of Moringa oleifera leaf extract used in the following experiments is the concentration of protein.

[0083] experimental method

[0084] 1. Preparation of samples according to the concentration of Moringa oleifera leaf extract

[0085] When 100 g of Moringa oleifera leaf dry powder ...

experiment example 1

[0113] Experimental example 1: Confirmation of the inhibitory effect of Moringa oleifera leaf extract on cancer cells in vitro

[0114] 1. The cancer cell inhibitory effect of Moringa oleifera leaves according to the extraction temperature and extraction time

[0115] In order to confirm the effect of Moringa oleifera leaf extract on cancer cells according to the extraction temperature and extraction time, each cancer cell was treated with the Moringa oleifera leaf extract prepared according to the above example at different concentrations, and then MTT analysis was carried out as follows The survival rate of cancer cells was confirmed.

[0116] In the 96-well plate medium, 3×10 per 100 μl was used as the basis 3 concentration to inoculate cells. After culturing for one day, each culture plate was treated with the Moringa oleifera leaf extract prepared in Example 1 at different concentrations of 0-400 μg / ml. After continuing to culture for 1 or 2 days, add 10 μl of Cell Cou...

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Abstract

The invention relates to a composition containing a moringa oleifera leave water soluble extract as an effective component and used for treating or preventing cancer, and preparation method for the moringa oleifera leave extract. The composition of the invention has low toxicity for normal cells but excellent toxicity for cancer cells. In addition, the composition is water soluble, so that the composition can be very easily absorbed in the cells, and accordingly the composition has beneficial advantages as oral preparations of an anti-cancer therapeutic agent; and the composition can be applied to development of natural anti-cancer new drug.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for treating or preventing cancer containing a water-soluble extract of Moringa oleifera leaves as an active ingredient, a composition containing a water-soluble extract of Moringa oleifera leaves as an active ingredient, and an effective anticancer drug Preparation method of Moringa oleifera leaf extract. Background technique [0002] Moringa is a small shrub native to India But it is cultivated anywhere in the world, and it has been transplanted into the wild in areas where Moringa is widely used. There are 13 species of moringa trees in the Moringa family, of which the Indian traditional moringa is the most well-known. Indian traditional Moringa is a 4-8 meter high shrub that unfolds in an umbrella shape. It is characterized by deciduous leaves with a length of 30-70cm, white and fragrant flowers, green, leathery and hanging long triangular long pods, up to 30-40cm long, native to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A23L1/29A61K8/97A61K127/00A23L33/00
CPCA23L33/105A23V2002/00A23V2200/308A61K8/9789A61K36/48A61Q19/00
Inventor 郑一来
Owner KOREA ATOMIC ENERGY RES INST
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