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Preparation method for formamide phenazine biological fungicide

A technology of formamidophenazine and biological fungicides, which is applied in the field of preparation of fungicides, and can solve the problems of narrow bactericidal spectrum and the like

Inactive Publication Date: 2014-12-24
JIANGXI POROR CROPS ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Systemic fungicides generally have strong bactericidal power and narrow bactericidal spectrum, and some of them have specific selective toxicity to certain pathogenic bacteria

Method used

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  • Preparation method for formamide phenazine biological fungicide
  • Preparation method for formamide phenazine biological fungicide
  • Preparation method for formamide phenazine biological fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] A kind of preparation method of formamide phenazine biological fungicide comprises the following steps, and following substances are expressed in parts by mass:

[0096] (1) Take the vegetative body of the cfcc 10466 Pseudomonas aeruginosa strain with the preservation number in the growth stage, and irradiate it with an ultraviolet lamp with a wavelength of 280 nanometers for 12 hours to obtain a mutant strain;

[0097] (2) Mix 2 parts of soybean powder, 0.2 parts of yeast powder and 50 parts of tap water, heat to 90°C, then filter, add 3 parts of tap water to the obtained filter residue, and continue to filter once;

[0098] (3) Add 0.3 part of glucose, 0.25 part of peptone, 0.04 part of potassium dihydrogen phosphate, 0.02 part of magnesium sulfate to the filtered filtrate, stir and dissolve, then add 1.2 parts of cornstarch and 5.3 parts of tap water to it, mix to obtain the solution, Use a sodium hydroxide solution with a molar concentration of 5 mol / L to adjust th...

Embodiment 2

[0126] A kind of preparation method of formamide phenazine biological fungicide comprises the following steps, and following substances are expressed in parts by mass:

[0127] (1) Take the vegetative body of the cfcc 10466 Pseudomonas chloropinus strain with the preservation number in the growth stage, and place it in an ultraviolet lamp with a wavelength of 300 nanometers for 15 hours to obtain a mutant strain;

[0128] (2) Mix 2 parts of soybean powder, 0.2 parts of yeast powder and 55 parts of tap water, heat to 95°C, then filter, add 3 parts of tap water to the obtained filter residue, and continue to filter twice;

[0129] (3) Add 0.35 parts of glucose, 0.30 parts of peptone, 0.06 parts of potassium dihydrogen phosphate, 0.03 parts of magnesium sulfate to the filtered filtrate, stir and dissolve, then add 1.5 parts of cornstarch and 5.3 parts of tap water, mix to obtain the solution, Use a sodium hydroxide solution with a molar concentration of 6mol / L to adjust the pH v...

Embodiment 3

[0157] A kind of preparation method of formamide phenazine biological fungicide comprises the following steps, and following substances are expressed in parts by mass:

[0158] (1) Take the vegetative body of the cfcc 10466 Pseudomonas chloropinus strain with the preservation number in the growth stage, and place it in an ultraviolet lamp with a wavelength of 320 nanometers for 18 hours to obtain a mutant strain;

[0159] (2) Mix 2 parts of soybean powder, 0.2 parts of yeast powder and 60 parts of tap water, then heat to 90-100°C, then filter, add 3 parts of tap water to the obtained filter residue, and continue to filter twice;

[0160] (3) Add 0.4 part of glucose, 0.35 part of peptone, 0.07 part of potassium dihydrogen phosphate, 0.04 part of magnesium sulfate to the filtered filtrate, stir and dissolve, then add 1.8 parts of cornstarch and 5.3 parts of tap water, mix to obtain the solution, Use a sodium hydroxide solution with a molar concentration of 7mol / L to adjust the ...

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Abstract

The invention discloses a preparation method for formamide phenazine biological fungicide. The preparation method comprises the steps of (1) irradiating pseudomonas chlororaphis vegetation under ultraviolet light to obtain mutant strains; (2) mixing soybean powder, yeast powder and tap water, heating and filtering; (3) adding glucose, peptone, potassium dihydrogen phosphate and magnesium sulfate in the filtrate, dissolving with stirring, regulating a pH value with sodium hydroxide, loading into a triangular flask and heating to a temperature of 120-125 DEG C for sterilization; (4) inoculating the mutant strains on a loop-ramp lawn to obtain strains containing formamide phenazine single component; and (5) screening strains with the highest content of the formamide phenazine single component by using high performance liquid chromatography and drug-containing plate method. The formamide phenazine biological fungicide is suitable for preventing plant pathogenic fungi producing a lot of spores, such as fungi diseases caused by botrytis cinerea pers. and fulvia fulva; a conventional foliar spraying method is adopted to carry out field control; and control efficiency can reach 70-80%.

Description

technical field [0001] The present invention relates to a kind of preparation method of fungicide, especially relate to a kind of preparation method of caramamide phenazine biological fungicide. Background technique [0002] Fungicides are a class of pesticides used to prevent and control plant diseases caused by various pathogenic microorganisms, generally referring to fungicides. [0003] Different fungicides work in different ways. The fungicide applied to the surface of the plant before the pathogen infestation has a preventive and protective effect is called a protective fungicide or a protective agent; the fungicide that can eliminate the infected bacteria at the application site is called an eradicating fungicide; Those that are transmitted to the infected part of the body to eliminate the germs are called systemic fungicides. Many eradicating agents are also systemic agents, and most of them have chemical therapeutic effects. Therefore, in practice, fungicides are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/60A01P3/00
Inventor 徐福祥
Owner JIANGXI POROR CROPS ENG
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