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A kind of tissue culture rapid propagation method of purple pearl

A tissue culture and purple bead technology, applied in the field of agricultural biology, can solve the problems of inability to meet the needs of production by seeding and propagation, low efficiency of cutting propagation, long dormant time of seeds, etc., to achieve fast propagation speed, no seasonal restrictions, and high survival rate Effect

Active Publication Date: 2016-03-16
四川立德种苗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the propagation of purple pearls is mainly concentrated on seed propagation and cutting propagation, but these two propagation methods are limited by seasons, and seed propagation has the disadvantages of low germination rate and long seed dormancy time, while cutting propagation efficiency is low, so , neither seed propagation nor cutting propagation can meet the needs of production

Method used

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  • A kind of tissue culture rapid propagation method of purple pearl
  • A kind of tissue culture rapid propagation method of purple pearl
  • A kind of tissue culture rapid propagation method of purple pearl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: A kind of tissue culture rapid propagation method of purple pearl, it comprises the following steps:

[0025] S1. Seed collection and pretreatment: collect the Zizhu fruit of no disease-free branches at noon on a sunny day every September, remove the Zizhu peel with tweezers and blades after drying in the shade, select healthy seeds, and set aside;

[0026] S2. Sterilization of seeds: Rinse the above seeds in running water for 1 hour, blot the excess water on the surface of the seeds with filter paper, sterilize the seeds in 75% alcohol for 25 seconds, rinse them twice with sterile water, and then use 0.1% Sterilize with mercuric chloride for 15 minutes, rinse with sterile water for 5 times, and use filter paper to absorb excess water on the surface of the seeds;

[0027] S3. Primary culture: inoculate the sterilized seeds on the primary culture medium, and switch to light culture after 6 days of dark culture. The culture temperature of light culture is 20°...

Embodiment 2

[0031] Example 2: A kind of tissue culture rapid propagation method of purple pearl, it comprises the following steps:

[0032] S1. Collection and pretreatment of seeds: collect the Zizhu fruit of no diseased branches at noon on a sunny day every October, remove the Zizhu pericarp with tweezers and blades after drying in the shade, select healthy seeds, and set aside;

[0033] S2. Sterilization of seeds: Rinse the above seeds in running water for 2 hours, blot the excess water on the surface of the seeds with filter paper, sterilize the seeds in 75% alcohol for 35 seconds, rinse them with sterile water for 3 times, and then use 0.1% Sterilize with mercuric chloride for 25 minutes, wash with sterile water for 7 times, and use filter paper to absorb excess water on the surface of the seeds;

[0034] S3. Primary culture: inoculate the sterilized seeds on the primary culture medium, and switch to light culture after 10 days of dark culture. The culture temperature of light cultu...

Embodiment 3

[0038] Example 3: A kind of tissue culture rapid propagation method of purple pearl, it comprises the following steps:

[0039]S1. Seed collection and pretreatment: collect the Zizhu fruit of no disease-free branches at noon on a sunny day every September, remove the Zizhu peel with tweezers and blades after drying in the shade, select healthy seeds, and set aside;

[0040] S2. Sterilization of seeds: Rinse the above seeds in running water for 1.5 hours, blot the excess water on the surface of the seeds with filter paper, sterilize the seeds in 75% alcohol for 28 seconds, rinse them twice with sterile water, and then use 0.1% mercuric chloride for 18 minutes, rinsed with sterile water for 6 times, and dried the excess water on the surface of the seeds with filter paper;

[0041] S3. Primary culture: inoculate the sterilized seeds on the primary culture medium, and switch to light culture after 7 days of dark culture. The culture temperature of light culture is 24°C, the ligh...

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Abstract

The invention discloses a tissue culture rapid propagation method of Auranthus japonica, comprising S1. collection and pretreatment of seeds: collecting Auranthus japonica fruit, selecting healthy seeds after drying in the shade, for standby; S2. sterilizing treatment of seeds: using Alcohol and mercuric chloride sterilization; S3. Primary culture: Inoculate the sterilized seeds on the primary culture medium, cultivate in the dark for 6-10 days and then switch to light culture for 13-18 days; S4. Subculture: Inoculate the germinated seeds Transfer to subculture medium and light culture for 28-32 days; S5. Proliferation culture: Cut Zizhu seedlings into stems with one leaf and one node, inoculate them on proliferation medium and light-culture for 13-18 days to obtain Zizhu group Cultivating seedlings; S6. Hardened seedlings and transplanting: transplant the purple bead tissue cultured seedlings into the mixed matrix of perlite and humus after hardening. The method of the invention has simple operation, no seasonal restriction, high reproduction rate, fast reproduction speed, high survival rate of transplanted seedlings, can provide a large amount of purple bead seedlings in a short time, and satisfies the production needs of landscaping and traditional Chinese medicine resource development.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a rapid propagation method of purple pearl by tissue culture. Background technique [0002] Purple Pearl (CallicarpabodinieriLevl.), also known as Baitangzi tree, Bauhinia, Purple Pearl Grass, and Hemostatic Grass, is a deciduous shrub of Verbenaceae and Purple Pearl. The plant height is about 1.2 meters to 2 meters. The branchlets are smooth and slightly purple. There are a small amount of stellate hairs, the single leaves are opposite, the leaves are obovate to elliptic, 7 cm to 15 cm long, the apex is acuminate, and the edges are sparsely serrate. Cymes axillary, with stalks, many flowers, buds purple or pink, flowers white, pink, lavender and other colors, open from June to July. The fruit is spherical, purple and shiny after ripening from September to October, and does not fall through winter. [0003] The morphological characteristics of purple pearl ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑超马建华王小辉朱晓菲沈香兰吴佳川
Owner 四川立德种苗科技有限公司
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