Special biological organic fertilizer for iron-rich green vegetables as well as preparation method and application of fertilizer
A bio-organic fertilizer and organic fertilizer technology, applied in the application, fertilization device, fertilizer mixture and other directions, can solve the problems of frequent occurrence, rapid spread and aggravation of pests and diseases, and achieve the effects of increasing crop yield, promoting vegetable growth and improving nutritional quality.
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Embodiment 1
[0029] Embodiment 1 Preparation of special-effect microbial bacterial powder
[0030] 1. Strain preparation and activation
[0031] Bacillus amyloliquefaciens was inoculated on a solid beef extract medium plate and cultured at 30°C for 48h. The formula of beef extract solid medium used is: beef extract 2g / L, glucose 4g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 15g / L, pH=7.2-7.4, sterilized at 121°C for 20 minute.
[0032] Inoculate Trichoderma chrysogenum on potato dextrose medium and culture at 25°C for 48h. The formula of the potato glucose medium used is: potato 20g / L, glucose 20g / L, agar powder 15g / L, wash and peel the potato and cut into small pieces, add water to boil and filter with 2 layers of gauze, add to the filtrate Dextrose and agar powder, sterilized at 121°C for 20 minutes.
[0033] 2. Expand training
[0034] Pick a single colony of Bacillus amyloliquefaciens that grows well and inoculate it into the beef extract liquid medium for expanded cultur...
Embodiment 2
[0039] Example 2 The preparation method of special bio-organic fertilizer for iron-rich green vegetables
[0040] 1. Remove the edible fungus waste residue from the packaging bag, dry it, and grind it to 200 meshes after drying, and set it aside.
[0041] 2. Mix 150kg of humic acid and 50kg of rapeseed meal with 750kg of the above-mentioned crushed edible mushroom waste residue, and adjust the water content to about 50%.
[0042] 3. Add 0.2% conventional organic material starter by weight of the mixture to the above mixture to ferment. The mixture was stacked 1 meter high, and bioaerobic solid fermentation was carried out for 7 days at normal temperature. During the fermentation process, the fermentation temperature is well controlled, and when the temperature reaches 50-70°C, turn over the pile. Then carry out the second fermentation, turning and throwing once every 3 days until it is completely decomposed, and the second fermentation time is 15 days.
[0043] 4. Dry the f...
Embodiment 3
[0046] Example 3 Field Fertilizer Effect Test of Iron-rich Green Vegetable Special Biological Organic Fertilizer
[0047] The test site is in Pingba, with a mild climate and abundant rainfall; the test soil belongs to paddy soil, infiltrated paddy soil subtype, infiltrated gray-brown tidal field soil, and gray-brown tidal sand mud field soil type (U322), and the soil layer is relatively shallow , the texture of the plow layer is sandy medium soil, and the soil fertility level is medium.
[0048] Test crops: lettuce (variety is Zhutongqing).
[0049] Experimental scheme: The experiment set up 4 treatments and 3 repetitions, arranged in random blocks. The residential area is 6.00 meters long, 3.33 meters wide, and covers an area of 20.0 square meters. The cultivation specification is 1.2 feet x 1.2 feet, 144 plants are planted in each plot, and 4,800 plants are planted per mu. Fertilization is done as follows:
[0050] 1. the bio-organic fertilizer+decrease 20% fertilizati...
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