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A stable uric acid detection kit

A technology for detection kits and reagents, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of poor sensitivity, cumbersome operation, poor specificity, etc. Low cost, accurate results, easy to operate effects

Active Publication Date: 2017-07-07
上海睿康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has been used for a long time, but it has poor specificity and poor sensitivity
At present, the more advanced blood uric acid determination methods are high-pressure liquid chromatography (HPL.C) and mass spectrometry. This method has the highest sensitivity, strong specificity, and accurate values. However, this method requires the use of independent instruments, and the operation is relatively cumbersome.

Method used

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  • A stable uric acid detection kit
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  • A stable uric acid detection kit

Examples

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Embodiment 1～3

[0036] The present examples 1-3 relate to a stable uric acid detection kit, which consists of reagent R1 and reagent R2. The components and concentrations of the reagents R1 and R2 are shown in Table 1:

[0037] Table 1

[0038]

[0039]

[0040] The UA detection kit described in Examples 1-3 of the present invention is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 2. Analysis method: Two-point endpoint method, that is, the amount of reagent R1 and R2 is 240ul and 80ul respectively, and the sample volume is 5ul; add 5ul sample to 240ul reagent R1 and incubate at 37°C for 5min, read the absorbance A1, add 80ulR2, and react for 5 minutes, Read the absorbance A2; the detection wavelengths are respectively the main wavelength 600nm and the secondary wavelength 800nm.

[0041] Adopt this reagent and above-mentioned assay method, adopt the curve of the UA standa...

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Abstract

The invention provides a stable uric acid detection kit. The stable uric acid detection kit is composed of a reagent R1 and a reagent R2, wherein the reagent R1 contains buffer solution, ethylenediamine tetraacetic acid disodium salt, magnesium acetate tetrahydrate, saccharose, an anti-interference agent, chromogen, a stabilizing agent and a preservative; the reagent R2 contains buffer solution, ethylenediamine tetraacetic acid disodium salt, 4-amino-antipyrine, peroxidase, uricase, a stabilizing agent and a preservative. The stable uric acid detection kit is used for measuring the content of blood uric acid by a colorimetric method that uricase is utilized to reduce uric acid; the method has strong specificity, has sensitivity higher than that of the chemical method, can be used for a full-automatic biochemical analyzer and can be applied clinically; and the anti-interference agent is added in the kit, the interference of ascorbic acid in the serum can be effectively removed, and the result is accurate.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis of medical immunity, in particular to a stable uric acid detection kit. Background technique [0002] The genetic material DNA (deoxyribonucleic acid) in the nucleus of a biological cell and RNA (ribonucleic acid) in the cytoplasm are composed of hundreds of thousands, millions or even tens of millions of nucleotides. Conversely, when nucleic acid is oxidized and decomposed, one of the products is purine, so purine is a component of cells. Old cells in the body, as well as food, especially foods rich in purine (such as animal offal, seafood, etc.), in the metabolic process in the body, the nucleic acid oxidation and decomposition products are purine (this endogenous purine accounts for the total purine 80% of After purine is produced in the body, it will be oxidized again in the liver to (2,6,8-trioxurine), also known as uric acid. 2 / 3 uric acid is excreted through the kidneys with urine, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 李伟奇李杰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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