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Preparation method of magnetic micro/nano gel-coated biological charcoal immobilized lipase

A technology of immobilizing lipase and biochar, which is applied in the direction of immobilization on or in the inorganic carrier, to achieve the effects of reversible regulation, low cost and simple application process

Inactive Publication Date: 2014-12-10
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1
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Problems solved by technology

However, there are no public reports on the use of biochar carriers for immobilized lipase, especially the combination of magnetic and temperature-sensitive functional materials.

Method used

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  • Preparation method of magnetic micro/nano gel-coated biological charcoal immobilized lipase

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Embodiment Construction

[0014] The implementation of the present invention will be described in detail below in conjunction with specific examples, so as to fully understand and implement the process of how to apply technical means to solve technical problems and achieve technical effects in the present invention.

[0015] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are also commercially available. The sources, trade names and, where necessary, listing of constituents of reagents used are indicated the first time they appear.

[0016] Embodiments of the invention are:

[0017] refer to figure 1 , a preparation method of biochar-immobilized lipase coated with magnetic nanogel, the specific operation steps are as follows: 1) synthesized into magnetic Fe 3 o 4 Granules: Dissolve 30.32g of ferric chloride hexahydrate and 15.6 g of ferrous sulfate heptahydrate in 100ml of deionized...

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Abstract

The invention provides a preparation method of a magnetic micro / nano gel-coated biological charcoal immobilized lipase. The method comprises the following steps: compounding magnetic ferroferric oxide nanoparticles and a sodium oleate solution to form a nano compound, mixing the nano compound with a temperature-sensitive monomer N-isopropyl acrylamide, a crosslinking agent and an emulsifier solution, and carrying out emulsion polymerization reaction to prepare the magnetic micro / nano gel; regulating the temperature of the solution according to the phase change characteristics of the obtained micro / nano gel, so that the micro / nano gel forms a coating on the surface of the biological charcoal; mixing the biological charcoal carrier with a free lipase solution so that the lipase molecules permeates into the micro / nano gel network of the carrier surface and the catalytic active site of the lipase is activated through the oil / water interface; and carrying out glutaraldehyde crosslinking reaction to immobilize the active lipase. The prepared magnetic immobilized lipase has higher catalytic activity, and can be conveniently separated and recovered from the reaction medium by the aid of a magnet, so the application cost is low.

Description

technical field [0001] The invention relates to the technical field of lipase immobilization, in particular to a preparation method of biochar-based immobilized lipase coated with magnetic micro / nano glue. Background technique [0002] Traditional lipase immobilization carriers often use inert inorganic or organic materials (introduced in Chinese Patent Publication Nos. CN102191236A, CN101613690A, CN101381721A, CN101280297A). High lipase catalytic activity. Compared with traditional inert carriers, intelligent polymer materials (such as temperature-sensitive hydrogels) are an ideal carrier for enzyme immobilization, which can increase enzyme loading, maintain enzyme molecular conformation and catalytic activity, and improve immobilization. Enzyme reusability and other aspects have important application value. Our previous studies have shown that poly-N-isopropylacrylamide hydrogel and its copolymers are a class of temperature-sensitive smart hydrogels (Hu et al., Journal o...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 胡朝华张蕾万顺刚
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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