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Protein peptide vaccine carrying system and preparing method thereof

A protein polypeptide and drug-carrying system technology, applied in the field of biomedicine, can solve problems such as high toxicity, and achieve the effect of improving expression or secretion and improving immune response effect.

Inactive Publication Date: 2014-12-10
WUHAN INST OF BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, organic solvents such as dichloromethane and chloroform are more toxic
At present, there is no report on the use of PS coated on the surface of PLA nanoparticles as a vaccine carrier

Method used

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  • Protein peptide vaccine carrying system and preparing method thereof
  • Protein peptide vaccine carrying system and preparing method thereof
  • Protein peptide vaccine carrying system and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: OVA / PLA / PS nanocapsule (containing 25mg / ml OVA) preparation

[0044] 1. Weigh 250mg OVA, dissolve it in PBS with pH 7.0, 0.01mol / L, and set the volume to 10.0ml, as the inner water phase.

[0045] 2. Weigh 125mg of PLA, dissolve it in 10ml of dichloromethane, stir well until uniform, and use it as the organic phase.

[0046] 3. Take 3ml of the organic phase prepared in step 2, add 450 μl of the internal aqueous phase prepared in step 1 dropwise to the organic phase, and stir to form a suspension.

[0047] 4. Using the JY99-2D ultrasonic cell breaker produced by Ningbo Xinzhi Biotechnology Research Institute, insert an ultrasonic probe into the suspension prepared in step 3, set the ultrasonic breaker to a success rate of 200W, an ultrasonic time of 3 seconds, and an interval of 5 seconds , sonicated 7 times to prepare colostrum.

[0048] 5. Weigh 100-300 mg of polyvinyl alcohol (PVA), dissolve it in pH 7.0, 0.01 mol / L phosphate buffered aqueous solution ...

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Abstract

The invention provides a polylactic acid-cladded protein peptide vaccine, a protein peptide vaccine carrying system for a protamine-cladded polylactic acid nanocapsule and a preparing method of the carrying system. Ovalbumin (OVA) is used as a model protein peptide vaccine, and an OVA solution is dispersed in a PLA-containing methylene dichloride organic phase by a probe ultrasonography method to form a primary emulsion. The primary emulsion is added into a polyvinyl alcohol containing external aqueous phase and treated by probe ultrasonography to form a multiple emulsion. A cured OVA / PLA microcapsule is obtained after the methylene dichloride organic phase is fully volatilized through agitating. Protamine (PS) is added into an OVA / PLA microcapsule suspension and agitated to obtain an OVA / PLA / PS microcapsule. The OVA / PLA / PS microcapsule has stable nanometer grain size, stable dispersing coefficient and surface potential suitable for transfect cells. The extracting capability of bone marrow derived dendritic cells (BMDC) for the OVA / PLA / PS microcapsule can be effectively improved. The expression of BMDC surface molecules MHCI, MHCII, CD83 and CD86 is obviously improved, and the secretion of a BMDC polarizing factor IL-12p70 is increased.

Description

technical field [0001] The invention relates to a protein polypeptide vaccine drug delivery system and a preparation method thereof, belonging to the field of biomedicine. Background technique [0002] Vaccines are autoimmune preparations made from pathogenic microorganisms (such as bacteria, rickettsia, viruses, etc.) and their metabolites, which are artificially attenuated, inactivated, or genetically engineered to prevent infectious diseases. Vaccines retain the property of pathogenic microorganisms to stimulate the immune system of animals. When the animal body comes into contact with this harmless pathogenic bacteria, the immune system will produce certain protective substances, such as specific antibodies, immune hormones, active physiological substances, etc.; when the animal is exposed to this pathogenic microorganism again, the animal body will The immune system will follow its original memory and produce more protective substances to prevent the damage of pathogen...

Claims

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Application Information

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IPC IPC(8): A61K39/385A61K39/29A61K38/16A61K9/51A61K47/42A61K47/34
Inventor 朱俊铭杨萍韩淑贤
Owner WUHAN INST OF BIOTECH
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