Application of boletus edulis in reduction of nitrite in animal blood
A delicious boletus and nitrite technology, which can be used in applications, animal feed, animal feed, etc., and can solve problems such as increased nitrite content
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Embodiment 1
[0043] Embodiment 1, delicacy boletus aqueous extract freeze-dried powder is to the reducing effect of nitrite content in rat blood
[0044] One, the preparation of delicious boletus extract
[0045] Take 500g of fruiting bodies frozen at -20°C for one year, add 500ml of distilled water, thaw at 15-25°C, grind to 50-65 mesh with a tissue grinder at 4°C, and extract at 4°C for 30 minutes. The extract was centrifuged at 8000g at 4°C for 15min, and the obtained supernatant (750ml) was the water extract of Boletus deliciosa, and 1.5ml of the water extract of Boletus deliciosa was obtained per gram of fruiting body of Boletus deliciosa.
[0046] Freeze the water extract of delicious boletus at -80°C, and then freeze-dry it with a lyophilizer to obtain 25,000 mg of delicious boletus water extract freeze-dried powder, with an average of 50 mg of delicious boletus fruiting body per gram of delicious boletus Aqueous extract lyophilized powder.
[0047] 2. The effect of freeze-dried p...
Embodiment 2
[0055] Embodiment 2, delicacy boletus (Boletus edulis) water extract freeze-dried powder capsule is to the reduction effect of nitrite content in rat blood
[0056] The delicious boletus water extract freeze-dried powder that step 1 obtains in embodiment 1 is filled boletus gelatin capsule, and each boletus gelatin capsule on average contains 0.02g delicious boletus water extract freeze-dried powder, obtains delicious boletus water Extract freeze-dried powder capsule.
[0057]Taking 30 male SD rats with a body weight of 200-220 g as experimental materials, the 30 male SD rats were randomly divided into two groups, each group having 15 male SD rats, namely the experimental group and the control group.
[0058] Each male SD rat of the experimental group is fed with 2 delicious boletus water extract freeze-dried powder capsules, and the oral dose is 18.2-20mg delicious boletus water extract freeze-dried powder / 100g body weight (with implementation The intragastric dose of the lo...
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