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A kit for detecting esophagus cancer related risky genes

A kit and esophageal cancer technology, applied in the fields of molecular biology and medicine, can solve the problems of abnormal riboflavin transport, affecting riboflavin utilization, increasing the risk of esophageal squamous cell carcinoma, etc.

Inactive Publication Date: 2014-12-03
浙江爱易生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

C20orf54 gene mutation may be an important reason for abnormal riboflavin transport and affecting riboflavin utilization
Riboflavin deficiency increases risk of esophageal squamous cell carcinoma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Use of detection kits

[0034] Step 1: Extraction of DNA template

[0035] Vacuum blood collection tubes are used to collect human peripheral blood, and the genomic DNA in the blood is extracted.

[0036] Step 2: Real-time quantitative PCR reaction

[0037] Use a fluorescent quantitative PCR detection kit that can detect risk genes related to esophageal cancer, which includes the following primer pairs and fluorescent probe pairs:

[0038] Sense Primer 1: 5'- ATGTGGAACGAGC – 3' (SEQ ID NO: 1)

[0039] Antisense primer 1: 5'- AAATACAAGATCA - 3' (SEQ ID NO: 2)

[0040] Sense primer 2: 5'-GTTCTGACCAGGG– 3' (SEQ ID NO: 3)

[0041] Antisense primer 2: 5'-GAGGAGATGGCCC- 3' (SEQ ID NO: 4)

[0042] Sense Primer 3: 5'- GCTGGTAAACAGT – 3' (SEQ ID NO: 5)

[0043]Antisense primer 3: 5'-CTGTGGTCTGGTG– 3' (SEQ ID NO: 6)

[0044] Sense Primer 4: 5'- GTGAGCATTAAAA - 3' (SEQ ID NO: 7)

[0045] Antisense primer 4: 5'-GTCAGTATAGGGA– 3' (SEQ ID NO: 8)

[0046] Sense pri...

Embodiment 2

[0073] Example 2. Services for esophageal cancer-related risk gene detection for patients

[0074] Step 1: DNA Extraction

[0075] The laboratory physicians of the hospital collect peripheral blood from the subjects, use vacuum blood collection tubes to collect peripheral blood, and extract genomic DNA from it.

[0076] Step 2: Genotyping Assays

[0077] Using the kit provided by the present invention, the rs2274223 SNP site on the PLCE1 gene, the rs13042395 SNP site on the C20orf54 gene, the rs2074356 SNP site on the C12orf51 gene, and the rs2014300 on the RUNX1 gene of the subject's genomic DNA SNP site No. 11066015 on the ACAD10 gene and SNP site rs10052657 on the PDE4D gene were detected by fluorescent quantitative PCR to determine the genotypes of these six SNPs sites.

[0078] Step 3: Analysis of individual risk of developing esophageal cancer

[0079] Through the analysis of the SNPs genotype of the tested subjects, an analysis report sheet of the risk of suffering f...

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PUM

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Abstract

A kit for detecting esophagus cancer related risky genes is disclosed. The kit includes: specific primer pairs, specific fluorescence probe pairs, common components for fluorogenic quantitative PCR detection; and the like, wherein the specific primer pairs and the specific fluorescence probe pairs are used for simultaneously detecting a number rs2274223 SNP site on a PLCE1 gene, a number rs13042395 SNP site on a C20orf54 gene, a number rs2074356 SNP site on a C12orf51 gene, a number rs2014300 SNP site on an RUNX1 gene, a number rs11066015 SNP site on an ACAD10 gene and a number rs10052657 SNP site on a PDE4D gene. The kit evaluates the risk of esophagus cancer for a person through simultaneously detecting single nucleotide polymorphic site genotypes of the PLCE1, the C20orf54, the C12orf51, the RUNX1, the ACAD10 and the PDE4D which are closely related with the esophagus cancer.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting risk genes associated with esophageal cancer, through simultaneous detection of genes PLCE1, C20orf54, C12orf51, RUNX1, ACAD10 and PDE4D associated with esophageal cancer Single nucleotide polymorphism locus genotype to assess the risk of individuals suffering from esophageal cancer. Background technique [0002] Esophageal cancer is one of the six most common malignant tumors in the world and ranks fourth among the causes of cancer-related death in China. Esophageal carcinogenesis is considered to be a multi-stage, progressive development process, in which both environmental and genetic factors play an important role. The role of various carcinogenic factors in the environment can change the structure and function of DNA in esophageal epithelial cells. At different stages of this change process, multip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/156
Inventor 任峻张华忠张路遥
Owner 浙江爱易生物医学科技有限公司
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