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Fragaria ananassa Duchesne fermented extract and preparation method and application thereof

The technology of extract and strawberry is applied in the field of cigarette additives, can solve problems such as no application, and achieve the effects of simple production process, convenient large-scale industrial production, and good development and application prospects.

Inactive Publication Date: 2014-12-03
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strawberry can be eaten raw and made into fruit wine, jam, etc., but there is no report of its application in cigarette filling in the prior art

Method used

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  • Fragaria ananassa Duchesne fermented extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Media and culture conditions

[0028] The Saccharomyces longans CGMCC 6645 used in the experiment was inoculated in 50 mL of YPD liquid medium, and cultured at 28°C and 160 r / min for 24 hours to obtain activated liquid strains.

[0029] 2. Preparation of Strawberry Samples

[0030] After crushing the strawberries, add 400mL of distilled water (20 times the volume), sterilize and set aside.

[0031] 3. Aroma-producing yeast fermentation treatment of strawberries

[0032] Inoculate 8 mL of the activated Saccharomyces longiosa solution prepared in 1 into the strawberry sample prepared in 2 (the inoculum size is 2%), and culture it with shaking at 28°C and 160r / min for 24 hours for the next experiment.

[0033] 4. Extraction of spice additives

[0034] After filtering the above fermented liquid, centrifuge at 8000r / min for 10 minutes, take the supernatant, concentrate it under reduced pressure at 50°C until it is in the form of an extract, and obtain the desired ferme...

Embodiment 2

[0047] Repeat Example 1, with the following differences:

[0048] The culture conditions for the activation of the strain CGMCC 6645 are 28°C, 160r / min shaking culture, and the culture time is 24 hours; add 22 times of distilled water to the crushed strawberries and sterilize them; inoculate the activated bacteria liquid on the strawberry material The dosage is 2.5%; the fermentation and cultivation time after inoculation is 24 hours; the supernatant of the fermentation broth is concentrated under reduced pressure at a temperature of 55°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 12g / box.

[0049] The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

Embodiment 3

[0051] Repeat Example 1, with the following differences:

[0052] The culture conditions for the activation of the strain CGMCC 6645 are 30°C, 180r / min shaking culture, and the culture time is 12 hours; after crushing the strawberries, add 15 times distilled water to sterilize; inoculate the activated bacteria liquid on the strawberry material The dosage is 1.5%; the fermentation and cultivation time after inoculation is 12 hours; the supernatant of the fermentation broth is concentrated under reduced pressure at a temperature of 45°C; the prepared spice additive is evenly sprayed on the shredded tobacco, and the addition amount is 8g / box.

[0053]The results of flavoring and smoking test show that the flavor additive can harmonize with the aroma of cigarettes, improve the quality of aroma, increase the amount of aroma, improve the aftertaste, cover up the miscellaneous smell, and have a fresh and special aroma.

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Abstract

The invention discloses a Fragaria ananassa Duchesne fermented extract and a preparation method and application thereof. The Fragaria ananassa Duchesne fermented extract is prepared from Fragaria ananassa Duchesne, which serves as a raw material, through fermenting with Lodderomyces elongisporus and extracting aroma components from a fermented solution. The Lodderomyces elongisporus is collected in the China General Microbiological Culture Collection Center (CGMCC); the collection number is CGMCC No. 6645; the collection date is October 08, 2012. The Fragaria ananassa Duchesne fermented extract contains a variety of aroma substances, such as phenylethyl alcohol, acetone, 4-ethyl guaiacol, methyl butyrate, 2-heptanone, ethyl caproate and the like. Shown by results of flavored smoking assessment tests, the product can be harmonized with cigarette flavors, can obviously promote the cigarette flavors, make smoke gas delicate and mellow, lower irritation, conceal impurity gases and improve the smoking flavor of cigarettes, and can generate a fresh and special aroma.

Description

technical field [0001] The invention belongs to the technical field of tobacco additives, and in particular relates to a tobacco flavor obtained by extracting aroma components from fermented strawberries. At the same time, the present invention also relates to the preparation method of the tobacco spice and its specific application in tobacco processing. Background technique [0002] Cigarette aroma is the core content of cigarette quality. Whether it is international or domestic, reducing the amount of tar has become an important issue in cigarette production, and with the reduction of the amount of tar, the taste of cigarettes becomes lighter and the aroma is weakened, which is difficult to be accepted by consumers. While reducing cigarette tar, it is an effective compensation method to increase and improve smoke concentration and flavor by adding spice additives. [0003] Natural flavors and fragrances are high-value fine chemical products, but the original natural flav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A24B3/12C12R1/645
Inventor 陈兴曾晓鹰段焰青王坚党立志黄立斌李庆华王家俊
Owner CHINA TOBACCO YUNNAN IND
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