Plant lodging and flower organ development regulation protein ZmLA1, and coding gene and application thereof

A gene and plant technology, applied to the plant lodging and flower organ development regulatory protein ZmLA1 and its encoding gene and application fields, can solve the problem of not participating in the regulation of genes at the same time, and achieve the effect of broad application prospects.

Active Publication Date: 2014-12-03
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although a large number of studies have clearly demonstrated the important role of auxin in the gravitational response and the development of floral organs, there are no reports on the regulation of genes involved in these two processes at the same time.

Method used

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  • Plant lodging and flower organ development regulation protein ZmLA1, and coding gene and application thereof
  • Plant lodging and flower organ development regulation protein ZmLA1, and coding gene and application thereof
  • Plant lodging and flower organ development regulation protein ZmLA1, and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, the acquisition of ZmLA1 protein and its coding gene

[0060]1. The acquisition of maize mutant ps1 and the genetic analysis of its mutant phenotype

[0061] 1. Obtaining the maize mutant ps1

[0062] The research team of the present invention took maize self-bred ensemble 31 (Hui Y, Guoying W, Jingrui D. Transformation of Maize Elite Inbred Lines [J]. Journal of Agricultural Biotechnology, 2001, 4:008.) as the background material, and transformed it through Mu9 The transposon system constructed a maize mutant library, and found that one of the mutants (named ps1) had two normal and mutant phenotypes in the BC3F2 segregation population through continuous backcrossing with Mo17:

[0063] Mutant type (hereinafter referred to as creeping phenotype or lodging phenotype): the aboveground part of the plant gradually tilts at the seedling stage, and shows a loss of gravity at the stage of 7-8 leaves, completely crawling (or lodging) on ​​the ground (such as fig...

Embodiment 2

[0117] Example 2, ZmLA1 mediates polar transport of auxin

[0118] 1. Determination of polar transport of auxin IAA to the base

[0119] The maize inbred line Mo17 was used as the recurrent male parent and the homozygous mutant ps1 was continuously backcrossed for three generations, and the BC3F2 population was obtained by selfing again. The seeds of the isolated creeping and erect individual plants were taken, cultured in the dark at 28°C for 3 days, the coleoptiles were taken, and the 2cm-long sections below the top 2mm were cut off, and every 0.5cm was marked with a marker pen. The intercepted section was put into 1 / 2 MS liquid medium, and rinsed on a shaker at 100 rpm for 2.5 hours to remove endogenous IAA. The surface liquid of the coleoptile section was blotted dry with filter paper, and the top of the coleoptile section was subjected to the following three treatments (each treatment was repeated three times, and each repetition was set with 5 coleoptiles):

[0120] Tr...

Embodiment 3

[0133] Example 3, ZmLA1 protein regulates the tissue development of plant vegetative growth and reproductive growth

[0134] The maize inbred line Mo17 was used as the recurrent male parent and the homozygous mutant ps1 for three consecutive generations, and then self-crossed, and the obtained BC3F2 population was planted in the field under normal water and fertilizer management. Strains were investigated for traits. The results shown in Table 9, compared with the individual plants of the erect phenotype, the plant height of the individual plants of the creeping phenotype is extremely significantly reduced; through the investigation of the loose powder and silking stages, the flowering stage of the male and female ears of the creeping individual plants is delayed by 5 days and 7 days respectively Above; Scanning electron microscope observation of male and female ears in differentiation, found that the spikelet primordium differentiation stage of the single plant of the creepin...

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Abstract

The invention discloses a plant lodging and flower organ development regulation protein ZmLA1, and a coding gene and an application thereof. The protein ZmLA1 is from Zea mays L., and is composed of an amino acid sequence represented by a sequence 1 in a sequence table. The protein or the gene can be used for regulating at least one of the following four properties of a target plant: the auxin-to-base transport efficiency, the auxin lateral transport efficiency, the stem lodging resistance ability and the flower organ development; and the flower organ growth properties comprise at least one of pistillate flowering, tassel flowering, tassel branch number, ear length, ear handle number of filaments, ear fresh weight, cluster length and fruiting rate. The gene and the protein provided by the invention have wide application prospects in the improvement of the properties directly relating to the plant steam lodging resistance, the ideal strain, the flower organ form and the like.

Description

technical field [0001] The invention relates to a plant lodging and flower organ development regulatory protein ZmLA1 and its coding gene and application. Background technique [0002] As the earliest plant hormone recognized by humans, auxin is widely involved in various stages of plant growth and development. The synthesis of auxin is mainly carried out in two ways: tryptophan-dependent pathway and tryptophan-independent pathway. Its synthesis parts mainly include newly formed tissues such as plant apical meristem, leaf primordia, germinated seeds and pollen. Compared with the characteristic of local region synthesis of auxin, auxin polar transport (PAT) is a more important reason for the uneven distribution of auxin. Unlike other plant hormones, auxin transport is carried out in a polar transport mode, that is, the transport direction in the body is one-way, and can only be transported from the upper end of the plant morphology to the lower end of the morphology. The a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C12N7/01C12N15/11A01H5/00
CPCC07K14/415C12N15/8261C12N15/827C12N15/8294
Inventor 金危危董朝斌陈晓阳
Owner CHINA AGRI UNIV
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