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Lipoteichoic acid from clostridium butyricum, and application thereof in adjusting immune response of livestock and poultry

A technology of lipoteichoic acid and Clostridium butyricum, which is applied in the direction of medical raw materials derived from bacteria, antibacterial drugs, drug combinations, etc., and can solve problems such as difficult implementation

Inactive Publication Date: 2014-11-26
NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Probiotics have the advantages of not causing bacterial resistance, no residue, and being environmentally friendly. However, the effect of probiotics is affected by various factors such as animal species, age, breeding environment, feed processing methods, and feeding methods. easy to implement

Method used

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  • Lipoteichoic acid from clostridium butyricum, and application thereof in adjusting immune response of livestock and poultry
  • Lipoteichoic acid from clostridium butyricum, and application thereof in adjusting immune response of livestock and poultry
  • Lipoteichoic acid from clostridium butyricum, and application thereof in adjusting immune response of livestock and poultry

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Embodiment Construction

[0028] Further describe the present invention in detail below by embodiment, but the present invention is not limited only to following embodiment.

[0029] 1. Extraction and preparation method of Clostridium butyricum lipoteichoic acid

[0030]Prepare 2% TX-114 solution with 50mmol / L, pH6.5 Tris-HCL buffer solution plus TritonX-114, store in refrigerator at 4°C, and set aside. Take 10mL of Clostridium butyricum cultured for 48 hours (anaerobic culture at 37°C, the medium is MRS medium, MRS medium formula: 10g casein peptone, 10g beef extract, 5g yeast powder, 20g glucose, 5g sodium acetate , Tween-801g, dipotassium hydrogen phosphate 2g, manganese sulfate heptahydrate 0.2g, manganese sulfate heptahydrate 0.05g, calcium carbonate 20g, distilled water 1000mL, pH7.0) in several plastic centrifuge tubes with known mass, at room temperature , Centrifuge at 5000rpm for 30min, weigh each tube after removing the supernatant culture solution, and calculate the wet weight of the bacte...

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Abstract

The invention discloses a lipoteichoic acid from clostridium butyricum and an application thereof in adjusting an immune response of livestock and poultry. The lipoteichoic acid is obtained by the steps of firstly preparing a TX-114 solution with a mass percentage of 1.5-2.5% for use; then layering 8-16 mL of a clostridium butyricum solution cultured for 45-50 h by centrifugation to obtain wet weight of clostridium butyricum; adding the TX-114 solution; centrifuging to obtain a crude extract of lipoteichoic acid; and then separating through column chromatography on a DEAE-cellulose chromatographic column. The lipoteichoic acid can improve intestinal immune functions of the livestock and poultry, suppress adhesion and attachment of pathogenic bacteria on surfaces of intestinal mucosal cells, reduce incidence of intestinal infectious diseases and increase economic benefits of livestock and poultry industry.

Description

Technical field: [0001] The invention relates to the cell wall lipoteichoic acid of clostridium butyricum which regulates the immune response of livestock and poultry, and the immune response is induced by pathogenic bacteria or their bacterial components. Background technique: [0002] Intestinal infectious disease is one of the most common infectious diseases in intensive livestock and poultry farming, mainly caused by pathogenic Escherichia coli, Salmonella, Staphylococcus aureus, Salmonella typhi, Vibrio cholerae and other pathogens. This type of disease is highly contagious and has a high mortality rate, which is very harmful to the livestock and poultry breeding industry. The pathogens of these intestinal infectious diseases may also spread from animals to humans, causing human intestinal diseases and posing a potential threat to the health of consumers. At present, the addition of antibacterial drugs to feed is still the main means of preventing and treating intestin...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P37/02A61P31/04
Inventor 王进波齐莉莉费明明梅乐和
Owner NINGBO INST OF TECH ZHEJIANG UNIV ZHEJIANG
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