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Vaccine for preventing toxoplasma infection as well as preparation method and application thereof

A technology for infection prevention and Toxoplasma gondii, which is applied in the fields of immunology and molecular biology, can solve the problems of not achieving the immune protection effect, achieve the effect of reducing the amount of worms and prolonging the survival time

Inactive Publication Date: 2014-11-19
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Although many vaccine candidate molecules have been revealed so far, none of them has achieved complete immune protection. Therefore, more and better DNA vaccine candidate antigens derived from different stages of Toxoplasma gondii, especially those related to the virulence of Toxoplasma gondii, have been found. Antigen gene is an important way to study vaccine against Toxoplasma gondii

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  • Vaccine for preventing toxoplasma infection as well as preparation method and application thereof
  • Vaccine for preventing toxoplasma infection as well as preparation method and application thereof
  • Vaccine for preventing toxoplasma infection as well as preparation method and application thereof

Examples

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Embodiment Construction

[0033] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0034] In the present invention, experimental materials are purchased from:

[0035] Female KM mice, about 6 weeks old, weighing 20±2g, were purchased from the Medical Experimental Center of Lanzhou University.

[0036] Host bacteria, Toxoplasma gondii RH, PRU strains, pVAX I eukaryotic expression vector and other materials were all preserved and provided by the Laboratory of Parasite Functional Genomics, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultu...

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Abstract

The invention firstly provides a nucleotide sequence for preventing toxoplasma infection. The nucleotide sequence is selected from one of the following nucleotide sequences: (1) a nucleotide sequence as shown in SEQ ID No.1 in a sequence table, (2) a nucleotide sequence which is complementary with the nucleotide sequence in (1) and (3) a nucleotide sequence which is obtained by substituting, deleting and modifying one or more basic groups of the nucleotide sequence as shown in (1) or (2) and has a function of preventing toxoplasma infection. The invention also provides a vaccine comprising the nucleotide sequence and a medically acceptable carrier. The plasmid DNA vaccine pVAX-ROP7 provided by the invention can be used for effectively preventing and controlling toxoplasma animal model (Kunming mouse) infection, namely effectively prolonging the survival time of a rat and reducing the polypide quantity of a brain cyst. Therefore, the plasmid pVAX-ROP7 can be used as a candidate antigen of DNA vaccine to prevent toxoplasma infection.

Description

technical field [0001] The invention belongs to the fields of immunology and molecular biology, and in particular relates to a vaccine for preventing toxoplasma gondii infection. Background technique [0002] Toxoplasma gondii (Toxoplasma gondii) is an obligate intracellular protozoa that parasitizes humans and various animals. It has a wide range of host species, including mammals, birds, reptiles, fish and humans. It can cause zoonosis Toxoplasmosis. Toxoplasma infection can lead to serious consequences such as miscarriage, teratogenicity or stillbirth in pregnant women; it is even more serious for immunosuppressed or immunodeficiency patients, and toxoplasmosis is one of the common causes of death. As an opportunistic infection agent, Toxoplasma gondii can cause severe organ damage in immunosuppressed or immunocompromised patients (such as AIDS patients, organ transplantation and malignant tumor patients, etc.). In addition, livestock and poultry diseases caused by Toxo...

Claims

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Application Information

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IPC IPC(8): C12N15/30C07K14/45C12N15/85A61K48/00A61K39/002A61P33/02C12R1/90
Inventor 徐民俊周洋袁子国宋慧群黄思扬周东辉陈佳
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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