Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

CHMM1 culture medium suitable for in-vitro culture of Chinese epidermal melanocytes

A CHMM1, melanocyte technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc., can solve the problems of poor cell growth, poor cell growth, and long waiting time in patients

Active Publication Date: 2014-11-19
HANGZHOU THIRD HOSPITAL
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] Therefore, some patients have to wait for a long time because of poor cell growth; another small number of patients have poor cell growth and cannot meet the needs of transplantation (Chen et al.1999, Show Chwan Med J19991:85; Chen et al., 2000, J. Dermatol 27:434)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • CHMM1 culture medium suitable for in-vitro culture of Chinese epidermal melanocytes
  • CHMM1 culture medium suitable for in-vitro culture of Chinese epidermal melanocytes
  • CHMM1 culture medium suitable for in-vitro culture of Chinese epidermal melanocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1.1 Materials and methods

[0063] 1.1.1 Preparation of medium

[0064] Add glutamine to Ham's F12 (GIBCO, Carlsbad, Calif.) to a final concentration of 2mM; penicillin-streptomycin to a final concentration of 100U / ml and 100μg / ml; fetal bovine serum (FBS, GIBCO, Carlsbad, Calif.) To a final concentration of 10% (volume / volume), culture medium A containing FBS and penicillin-streptomycin was obtained, stored at 4°C, and freshly prepared once every two weeks.

[0065] bFGF (PeproTech, Rocky Hill, N.J.) was dissolved in Ham’s F12 medium (GIBCO, Carlsbad, Calif.) at a concentration of 2500 ng / ml, and stored at -70°C after aliquoting. Once a week, the bFGF stock solution was added to medium A to a final bFGF concentration of 30 ng / ml.

[0066] GM-CSF (PeproTech, Rocky Hill, N.J.) was dissolved in Ham’s F12 medium at a concentration of 3000ng / ml, and stored at -70°C after aliquoting. Once a week, the GM-CSF stock solution was added to medium A to a final concentration of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a CHMM1 culture medium suitable for in-vitro culture of Chinese epidermal melanocytes. The CHMM1 culture medium takes a base culture medium suitable for in-vitro culture of the epidermal melanocytes as a matrix, wherein the matrix is added with the following components: (1) serum with the volume concentration of 5%-20%, (2) 1-100 ng / mL of bFGF and 1-100 ng / mL of GM-CSF, and (3) 1-100 [mu]g / mL of 3-isobutyl-1-methylxanthine (IBMX) and 3-3000 ng / mL of alpha-MSH. The invention provides the culture medium suitable for in-vitro culture of the Chinese epidermal melanocytes, and the culture medium can be used for culture of the epidermal melanocytes having strengthened proliferation and melanin synthesis abilities, so that the epidermal melanocytes are better applied in transplantation treatment of skin disease patients with hypopigmentation or no pigments.

Description

(1) Technical field [0001] The invention relates to a CHMM1 medium suitable for in vitro culture of Chinese epidermal melanocytes. (2) Background technology [0002] The change of human skin color is directly related to the number, size, type and distribution of melanosomes. Skin color is primarily determined by the combination of pigments manufactured by the skin and natural pigments contained in the top layers of the skin. The epidermis on the surface of the epithelial layer of the skin contains epidermal melanocytes that produce skin pigment (melanin). In people with hypopigmented or amelanotic skin conditions, the melanocytes in the skin are missing, damaged, or nonfunctional, resulting in white spots on various parts of the body. [0003] Melanin is a protein derivative produced by melanocytes in the epidermis. At present, it is believed that epidermal keratinocytes are involved in the formation and metabolism of skin melanin. Melanocytes and keratinocytes are collec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/071
Inventor 许爱娥林福全
Owner HANGZHOU THIRD HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com