Method for extraction, enrichment and quantification of trace ofloxacin on suspended particulate matter in water

Abstract

The invention discloses a method for extracting, enriching and quantifying trace ofloxacin on suspended particulate matters in water. The method comprises the following steps: filtering a target water sample by using a micro-pore fiber membrane; collecting the fiber membrane subjected to filtration; airing the fiber membrane and cutting the fiber membrane into fragments; putting the fragments into a triangular flask; adding an extracting agent and sealing the triangular flask; oscillating and extracting by ultrasonic waves; filtering extracting liquid by using an organic fiber membrane; meanwhile, transferring the filtered extracting liquid into a K-D concentration bottle; adding a dehydration drying agent into an extracted filtering liquid; after absorbing water, putting the K-D concentration bottle into a rotary evaporator and concentrating; blowing the concentrated liquid by use of nitrogen gas until the volume is smaller than 1mL; making up the volume of the concentrated extracted filtering liquid to be 1mL and transferring the concentrated extracted filtering liquid to an Agilent special bottle; detecting the liquid by adopting an internal standard method through a high performance liquid chromatography cascaded three-grade gas chromatograph-mass spectrometer; and analyzing a chromatograph-mass spectrum analysis pattern to finish detection. According to the method, the content of the ofloxacin adsorbed on the suspended particulate matters in a water environment can be supplemented and the blank of the detection of antibiotics can be filled.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a detection method of ofloxacin in a water environment, in particular to a quantitative method for extraction and enrichment of ofloxacin on particulate matter in water. Background technique [0002] Ofloxacin is a synthetic, high-efficiency, broad-spectrum antibacterial fluoroquinolone drug that is effective against Gram-positive bacteria (including methicillin-resistant Staphylococcus aureus) and Gram-negative bacteria (including Pseudomonas aeruginosa). Bacillus) have a strong effect, and also have certain activity against Mycoplasma pneumoniae, Neisseria, anaerobic bacteria and Mycobacterium tuberculosis. It inhibits DNA synthesis and replication by acting on the A subunit of bacterial DNA helicase, leading to bacterial death. [0003] Ofloxacin was synthesized in 1982. It is easy to absorb when taken orally, fast and complete, with high and long-lasting blood...

AI Technical Summary

Problems solved by technology

These methods are limited to the quantification of dissolved ofloxacin

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