Method for extraction enrichment and quantification of trace ciprofloxacin on suspended particles in water

A technology for suspending particles and water suspension, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2014-11-19
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

These methods are limited to the quantification of dissolved ciprofloxacin

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  • Method for extraction enrichment and quantification of trace ciprofloxacin on suspended particles in water
  • Method for extraction enrichment and quantification of trace ciprofloxacin on suspended particles in water

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[0034] The technical solution of the present invention will be further described below, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention .

[0035] Such as figure 1 As shown, the method for extracting, enriching and quantifying trace amounts of ciprofloxacin on suspended particles in water provided by the invention, its specific implementation steps are as follows:

[0036] (1) Take 2000ml of the target water sample, filter it with a 0.45μm microporous fiber membrane, collect the filtered filter membrane, dry the filter membrane, cut it into pieces and put it in the conical flask, measure an appropriate amount of methanol with a measuring cylinder and pour it into the conical flask In the above, the volume ratio of the mass of suspended parti...

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Abstract

The invention discloses a method for extraction enrichment and quantification of trace ciprofloxacin on suspended particles in water. The method comprises the steps: filtering a target water sample by a microporous fiber membrane, collecting the filter membrane after filtration, air-drying the filter membrane, then cutting into pieces, placing in a triangular flask, adding an extracting agent, sealing, oscillating, and carrying out ultrasonic extraction; filtering the extracted liquid by an organic filter membrane, and at the same time, transferring the filtered extracted liquid to an K-D concentration bottle; adding a dehydrating drying agent into the extracted filtrate, after absorbing up water, putting the K-D concentration bottle into a rotary evaporator, and concentrating; blowing the concentrated liquid with nitrogen gas until the volume is below 1 mL; making the concentrated extracted filtered liquid to a constant volume of 1 mL, and then transferring to an Agilent special flask; adopting an internal standard method, and detecting by a high performance liquid chromatography-tandem three-grade mass spectrometry instrument; and analyzing a chromatographic mass spectrometric analysis diagram, and thus completing the detection. The method supplements the content of ciprofloxacin adsorbed on the suspended particles in the water environment, and supplements a blank of antibiotic detection.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a method for detecting ciprofloxacin in a water environment, in particular to a quantitative method for extracting and enriching ciprofloxacin on particulate matter in water. Background technique [0002] Ciprofloxacin belongs to the third-generation fluoroquinolone antibiotic drug, and its antibacterial activity is the strongest among the fluoroquinolones widely used at present. Pneumococcus and Streptococcus are slightly less effective than Staphylococcus and can kill bacteria such as anthrax by interfering with enzymes that cause reverse transcription after DNA replication, preventing DNA and protein synthesis. [0003] Ciprofloxacin is widely used in clinical medicine. Due to incomplete metabolism in organisms, the drug concentration in excreta will still remain high. Conventional water and sewage treatment cannot completely reduce its concentration, and it wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/08
Inventor 徐勇鹏崔福义
Owner HARBIN INST OF TECH
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