A method for detecting the concentration of petroleum hydrocarbons in the ocean

Abstract

The invention discloses a method for detecting the concentration of petroleum hydrocarbons in the ocean by using D. coliformis, including the feeding of D. spp., the configuration of crude oil water-soluble solution, the configuration of petroleum hydrocarbon concentration gradient culture solution, and the exposure of petroleum hydrocarbons and sampling, single-cell gel electrophoresis analysis, and data analysis. This detection method has a wide range of applications, short experimental procedures, good safety, high sensitivity and accuracy, and can detect the coelom of D. pallidum at the single-cell level. The DNA damage of liquid cells is visualized and converted into the concentration of petroleum hydrocarbons in the ocean.

Description

technical field [0001] The invention relates to the field of research on the concentration of toxic substances in the marine environment, in particular to a biological detection method for the concentration of petroleum hydrocarbons in the ocean. Background technique [0002] Petroleum hydrocarbons are complex mixtures of hydrocarbons, mainly composed of hydrocarbons, especially light aromatic hydrocarbons and their derivatives. Petroleum hydrocarbons are toxic to marine organisms, mainly causing confusion in the nutrition and transport of organisms, and even lead to mass death of organisms in high concentrations, which can cause great damage to marine ecosystems. In particular, high-ring aromatic hydrocarbons are the most toxic. Some components are carcinogenic and can cause fish to produce oily odor. The main hazard is that it contains carcinogenic hydrocarbons, which will endanger human health through the food chain after being enriched by fish and shellfish. In recent ...

AI Technical Summary

Problems solved by technology

Due to its low sensitivity, ultraviolet spectrophotometry is ineffective for saturated hydrocarbons and cyclic hydrocarbons, so it is more suitable for the determination of petroleum hydrocarbon concentration in high-concentration samples

The gravimetric method has a long research process and low sensitivity. It is only suitable for the determination of water samples with an oil content above 10mg / L, and it is easy to volatilize during the extraction and solvent evaporation process. The precision of the method varies with the operating conditions and the proficiency of the operator. differ greatly

Infrared photometry includes two parts: non-dispersive infrared absorption photometry and infrared spectroscopy. The advantage of the former is that the instrument structure is simple and the measurement has good reproducibility. The disadvantage is that it can only study straight-chain alkanes and cycloalkanes in petroleum hydrocarbons Alkanes, benzene series cannot be studied, the latter can accurately measure the total concentration of petroleum hydrocarbons, but when determining the concentration of petroleum hydrocarbons in fish and shellfish, if there is animal oil in the fish and shellfish, it is very easy to be interfered

Fluorescence spectrophotometry has a very high sensitivity for studying the concentration of petroleum hydrocarbons, but it can only measure the concentration of benzene series in petroleum hydrocarbons, and cannot measure the concentration of straight-chain alkanes and straight-chain alkanes in petroleum hydrocarbons, and the fluorescence emission intensity of different substances is different Very large, the determination result is greatly affected by the oil composition in the sample

Gas chromatography is to separate the petroleum hydrocarbon chromatographic column and study different petroleum hydrocarbon components separately. It has high sensitivity, but due to the complex composition of petroleum hydrocarbons, it is not suitable for the determination of the total concentration of petroleum hydrocarbons.

Images