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Preparation method of artificial ligament modified by strontium/silk protein biomimetic coating

An artificial ligament and silk protein technology, applied in the field of biomedicine, can solve the problems of poor tendon-bone healing and low degree of healing between artificial ligament and tendon-bone, and achieve the effect of solving uneven coating

Active Publication Date: 2015-12-30
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the defects in the prior art, the purpose of the present invention is to provide a method for preparing artificial ligaments modified by strontium / silk protein biomimetic coating with simple preparation, mild conditions, energy saving and environmental protection, and good material histocompatibility. The low degree and slow speed of artificial ligament and tendon-bone healing exposed by the application
The invention utilizes the biomimetic mineralization method, uses silk protein as a mineralization template, and modifies the surface of the artificial ligament with a strontium biomimetic silk protein composite coating to form a composite coating with uniform mineralization structure and stable performance, thereby improving the artificial ligament in vivo. The problem of poor tendon-bone healing in the application

Method used

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  • Preparation method of artificial ligament modified by strontium/silk protein biomimetic coating
  • Preparation method of artificial ligament modified by strontium/silk protein biomimetic coating
  • Preparation method of artificial ligament modified by strontium/silk protein biomimetic coating

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Experimental program
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Effect test

Embodiment 1

[0034] Under the protection of nitrogen, with water as solvent, 0.1ml of 1mmol / L SrNO 3 Mix with 1ml of 50mM natural silk protein solution, adjust the pH value to 8, and stir at 25°C for 5min. 10 x 10 in background vacuum -5 Pa, power 50W, use H 2 The artificial ligament material was treated with plasma for 60 minutes, then washed with pure water and dried in vacuum to obtain the pretreated artificial ligament material.

[0035] Then soak the artificial ligament in the strontium-containing silk protein mixture, and stir for 30 minutes. Fully combine the free strontium ions in the system with the amino residues of silk protein, then take out the filter paper and blot dry, then soak in 1ml of 50mM silk protein solution, react at 25°C for 5min, repeat the above steps 5 times to obtain strontium / silk protein Novel artificial ligament modified with biomimetic coating.

[0036] Among them, the biological evaluation of the new artificial ligament modified by the obtained hydroxya...

Embodiment 2

[0042] Using water as a solvent, mix 1ml of 10mmol / L strontium chloride with 100ml of 100mM recombinant silk protein solution, adjust the pH value to 7.4, stir for 45 minutes at 25 degrees Celsius, and vacuum at 10×10 -5 Pa, power 100W, use N 2 The PET artificial ligament was treated with plasma for 60 minutes, then washed with pure water and dried in vacuum; then the artificial ligament was soaked in the mixture, and stirred for 20 minutes. Fully combine the free Si ions in the system with the amino residues of silk protein, then take out the filter paper to dry, and finally soak in 100 ml of 100mM recombinant silk protein solution, stir and react at 25 degrees Celsius for 45 minutes, repeat the above steps 30 times, A new artificial ligament modified with hydroxyapatite-doped strontium composite silk protein mineralization coating was obtained.

Embodiment 3

[0044] Under normal temperature and pressure, mix 0.5 ml of a mixture of 5 mM strontium nitrate and strontium chloride with 25 ml of 85 mM natural silk protein and recombinant silk protein mixed solution at 25 degrees Celsius for 15 minutes, adjust the pH value to 7.6, in this Bottom Vacuum 10×10 -2 Pa, power 80W, use O 2 Treat the PET artificial ligament with plasma for 30 minutes, then wash it with pure water and dry it in vacuum; then soak the artificial ligament in this mixture, stir and react for 25 minutes, so that the free Si ions in the system can fully combine with the amino residues of silk protein, and then Take out the filter paper to dry, and finally soak in 50 ml of 85mM silk protein solution, stir and react at 25 degrees Celsius for 15 minutes, repeat the above steps 15 times, and obtain a new type of artificial hydroxyapatite-doped strontium composite silk protein mineralization coating modification. ligament.

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Abstract

The invention discloses a method for preparing a strontium / fibroin bionic coating modified artificial ligament. The method comprises the following steps: 1, modifying a PET (polyethylene glycol terephthalate) artificial ligament; 2, immersing the pre-treated PET artificial ligament material in strontium-containing fibroin mixed liquor, stirring to react for 20-30 minutes, taking out and airing; putting the artificial ligament material into a fibroin solution, standing and airing; and 3, repeating the step 2 until the number of preset circulating times is achieved to obtain the strontium / fibroin bionic coating modified artificial ligament. According to the method, a biomimetic mineralization method is utilized, the fibroin is used as a mineralizing formwork, and strontium bionic fibroin composite coating modification is performed on the surface of the artificial ligament to form a composite coating with a uniform mineralized structure and stable performance, and the problem of poor tendon-bone healing of the artificial ligament in in-vivo application can be improved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a preparation method of artificial ligament modified by strontium / silk protein bionic coating. Background technique [0002] The selection of grafts for knee cruciate ligament injury reconstruction, many clinical studies have proved that polyethylene terephthalate (PET) artificial ligament has satisfactory short-term curative effect and safety, but it still has poor tendon-bone healing And concurrent synovitis and other problems. Therefore, it is particularly important to improve the biocompatibility of PET materials and promote tendon-bone healing at both ends of the artificial ligament bone canal. [0003] Since the surface of PET artificial ligament graft first comes into contact with the internal environment of the host, it has become the development of orthopedic biomaterials to carry out surface coating treatment to improve the hydrophilicity, biocompatibility and osteoconductivit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34A61L27/30A61L27/50
Inventor 陈世益张鹏陈俊蒋佳吴子英万方陈天午
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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