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Making method of human intestinal micro-ecological system

A micro-ecosystem and intestinal technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, unknown raw materials, etc., to achieve strong anti-interference ability and good healing effect.

Inactive Publication Date: 2014-10-22
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, few attempts have been made so far to study the impact of the human gut microbiome as a whole on its defense against external pathogens

Method used

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  • Making method of human intestinal micro-ecological system
  • Making method of human intestinal micro-ecological system
  • Making method of human intestinal micro-ecological system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation method of human intestinal micro-ecosystem

[0022] 1. Collect the first defecation sample of volunteers in the morning, transfer to anaerobic environment, add glycerol, the weight of which is 10% of the total weight of feces and glycerin, mix with a vortex shaker, and store at -80 °C.

[0023] 2. Assembly and use of the chemostat: Connect the storage tank, fermentation tank, waste liquid tank, rubber stopper, silicone tube, glass bottle containing NaOH and ventilation filter in order, place them on the workbench, connect and mix Gas, peristaltic pump, pH regulator, magnetic stirrer, exhaust gas removal device

[0024] 3. Prepare the medium for chemostat culture, based on a total volume of 500 ml, the mass percentages of the following components are: 5‰ soluble starch, 3‰ bovine casein, 3‰ peptone, 0.6‰ pectin, 0.6‰ xylan , 0.6‰ Arabinogalactan, 0.6‰ Amylopectin, 0.4‰ Cysteine, 0.01‰ Heme, 0.25‰ Cholesterol, 0.25‰ Chenodeoxycholic Acid, 0.25‰ Cholic Acid, 2...

Embodiment 2

[0026] Example 2: Therapeutic Effect of Intestinal Microecological System on Infectious Diseases Caused by Enrofloxacin

[0027] 1. Construct a chemostat according to step 1, step 2, step 3 and step 4 in Example 1, prepare medium and make human intestinal micro-ecosystem.

[0028] 2. After the chemostat runs stably for 23 days, add 1.25 μg / mL, 12.5 μg / mL, 125 μg / mL, and 125 μg / mL enrofloxacin respectively to create an antibiotic disorder model. Converter served as a control group.

[0029] 3. From the 17th to 31st day of cultivation, take 10 mL of the culture from each fermentation tank every day, and count the changes in the number of microorganisms in the culture by using the live bacteria timing method; Variety. The specific operations of viable count and denaturing gradient gel electrophoresis are as follows:

[0030] Viable bacteria count: samples were taken under aseptic conditions, after 10-fold dilution, cultured under suitable conditions, observed the growth of col...

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Abstract

The invention relates to a making method of a human intestinal micro-ecological system. The human intestinal micro-ecological system cultured by a chemostat is used to prevent and treat infectious diseases, the self-made chemostat is used to culture healthy volunteers having no antibiotic history within three months, and the human intestinal micro-ecological system is used for treating enrofloxacin infections diseases. Compared with single or several probiotic combination, the human intestinal micro-ecological system directly treating the whole micro-ecological system of the healthy volunteers as an integral body to prevent and treat diseases has the advantages stable composition, strong anti-interference ability and good treatment effect; and additionally, the micro-ecological system used in the invention originates from the human body, so the cultured micro-ecological system is safe to human bodies, and has no toxic side effects.

Description

technical field [0001] The invention relates to a preparation method of human intestinal microecological system. Background technique [0002] In the process of livestock breeding, the abuse of a large number of antibiotics leads to their residues entering the food supply channels, and finally entering the human body and causing infectious diseases. At present, the national standards for the use of antibiotics in China, Europe, Australia and the United States clearly require the safety evaluation of microbial flora. However, the abuse of antibiotics still exists. [0003] Enrofloxacin is a broad-spectrum antibacterial agent. In China, it is allowed to be used in livestock breeding, mainly through subcutaneous injection, intramuscular injection or oral administration to livestock. However, enrofloxacin residues will enter the human intestinal tract with the food supply and change the number and diversity of intestinal flora in the eaters, thereby affecting human he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/66A61P31/00
Inventor 陈廷涛辛洪波王鑫
Owner NANCHANG UNIV
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