Method for detecting cytotoxicity of nano materials through yeast mutant strains

A nano-material and cytotoxic technology, which is applied in the direction of microbial-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve problems such as high equipment and environmental requirements, small number of samples, and limited application, and achieve rapid More sensitive detection, fast detection speed, and improved sensitivity

Inactive Publication Date: 2014-10-15
INST OF AQUATIC LIFE ACAD SINICA
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two detection methods have their shortcomings that cannot be ignored. For example, for animal exposure experiments, the disadvantages include small number of samples, large individual differences, long exposure time, and high price; , the disadvantages are high technical requirements for operation, high requirements for equipment and environment, and the price of animal serum used is very expensive, etc.
These shortcomings limit the application of these two methods in actual production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting cytotoxicity of nano materials through yeast mutant strains
  • Method for detecting cytotoxicity of nano materials through yeast mutant strains
  • Method for detecting cytotoxicity of nano materials through yeast mutant strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for rapidly detecting cytotoxicity of nanomaterials by using yeast mutant strains, the steps of which are:

[0041] 1. cwp1cwp2pdr5snq2 Four gene knockout yeast strains and yap1cwp1cwp2pdr5snq2 Construction of a five-gene knockout yeast strain: (see patents CN102120968A "A baker's yeast highly sensitive to oxidative damage and its preparation method" and CN101717736A "an ultra-high permeability baker's yeast and its preparation method").

[0042] ① Extract the genome of Saccharomyces cerevisiae, the wild-type yeast used is BY4741, and the genotype is MaTahis3Δ1leu2Δ0met15Δ0ura3Δ0 (Purchased from the Saccharomyces Gene Deletion Project) (For the extraction method, refer to Xing Fuguo, Zhang Peijun, Tan Xungang, Xu Yongli, "Extraction of Yeast Genome", "Food Science", Issue 03, 2007);

[0043] Yeast genome extraction method: inoculate yeast into yeast YPD medium, culture at 30°C for 16-18 hours; collect bacteria by centrifugation; use SCED (1mol / L sorbitol, 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for detecting cytotoxicity of nano materials through yeast mutant strains. The method comprises the following steps: A, knocking out yeast specific genes: extracting saccharomyces cerevisiae genomes, building gene knockout components, transferring the gene knockout components into yeast cells through a lithium acetate conversion method and screening so as to obtain gene knockout yeast; B, preparing nano material suspension liquid; C, testing nano material through yeast; D, detecting concentration of cells after test through yeast; E, computing the result and analyzing: deducting blank, and computing the growth inhibition ratio/cell viability; F, according to the growth inhibition ratio of nano materials to yeast, obtaining half effective concentration EC50 of nano materials to yeast, and then judging toxicity of nano materials. The method is feasible, the operation is simple and convenient, the detection speed is high, the sensitivity is high, the cost is low, the application range is wide, and the method can be used for detecting cytotoxicity of nano materials to organisms in different aqueous solutions.

Description

technical field [0001] The invention belongs to the field of microbial toxicity detection of new materials, and more specifically relates to a method for rapidly detecting cytotoxicity of nanomaterials by using yeast mutant strains, which is suitable for environmental impact assessment of nanomaterials. Background technique [0002] Nanoscience is an emerging discipline developed in the late 1980s, and it is listed as the three most promising new technological fields in the 21st century along with information science and life science. Nanomaterials have superior magnetic properties, electrical conductivity, reactivity, and optical properties due to their nanoscale and nanostructure. These unique properties enable them to be widely used in many fields such as sensors, catalysts, superconductors, cosmetics, and environmental protection. At present, nano products have stepped out of the laboratory and entered people's lives. It is estimated that during 2011~2015, the total inve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/865
Inventor 方涛鲍少攀陆其聪唐巍戴和平
Owner INST OF AQUATIC LIFE ACAD SINICA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap