Establishment method for Fraxinus rhynchophylla Hance. suspension culture system

A system of Astragalus chinensis and system technology, applied in the field of plant cell engineering, to achieve the effects of promoting rapid reproduction, high dry matter content, and compact callus

Inactive Publication Date: 2014-10-15
NORTHEAST FORESTRY UNIVERSITY +2
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  • Claims
  • Application Information

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Problems solved by technology

Callus induced plant regeneration and the establishment of suspension culture system have not been reported yet

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  • Establishment method for Fraxinus rhynchophylla Hance. suspension culture system
  • Establishment method for Fraxinus rhynchophylla Hance. suspension culture system

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Embodiment Construction

[0015] A method for establishing a suspension culture system of Fraxinus mandshurica, its concrete steps are as follows:

[0016] Step 1: Take the stems and leaves of Astragalus as explants, soak them in 75% alcohol for 25s or 28s or 30s, and soak them with saturated Ca(ClO) 2 Treat with aqueous solution for 8 minutes or 10 minutes or 12 minutes, rinse with sterile water 4 times or 5 times or 6 times;

[0017] Step 2, cut the sterilized stems and leaves into 0.5-1cm (cm) in a petri dish with a sterile knife 2 ), inoculated on the solid starting medium, WPM medium supplemented with NAA (a-naphthalene acetic acid) 1.2-1.8mg / L, TDZ (thidiazuron) 0.05-0.1mg / L, sucrose 20g / L , Agar 5.3g / L, pH6.0~6.5;

[0018] Step 3, wait until the callus grows to 1cm ~ 2cm 3 , transferred to the subculture medium WPM medium supplemented with NAA1.0mg / L, TDZ (thidiazuron)0.1mg / L, sucrose 20g / L, agar 5.3g / L, pH6.0~6.5; light 16h a day, temperature 23℃ ~26°C, cultivate for 30 days;

[0019] Step...

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Abstract

Belonging to the technical field of cell engineering, the invention in particular relates to an establishment method for a Fraxinus rhynchophylla Hance. callus mass suspension culture system. The method includes: taking excellent Fraxinus rhynchophylla Hance. stems and leaves as raw materials, inducing calluses on a WPM solid medium added with hormone NAA and TDZ, and inoculating the calluses into a WPM liquid medium added with hormone NAA and TDZ to undergo illumination shaking culture. After multi-generation directional induction domestication, the callus mass suspension system characterized by stable growth, rapid proliferation and uniform mass can be established. The method provided by the invention has the characteristics of simplicity and practicability, strong operability and considerable proliferation coefficient, and can promote rapid propagation of Fraxinus rhynchophylla Hance.

Description

technical field [0001] The invention relates to a method for establishing a suspension culture system of Aspergillus fruticosa by using a basic medium and additional hormones, and the invention belongs to the technical field of plant cell engineering. Background technique [0002] Fraxinus rhynchophylla Hance. belongs to the large deciduous tree of the genus Ash in the family Oleaceae, also known as the big-leaved white wax tree and the bitter mantle white wax tree. Dried bark and branch bark are one of the four medicinal skins of the traditional Chinese medicinal material "Qinpi", which has the functions of clearing away heat and dampness, improving eyesight, relieving cough and relieving asthma. Analytical chemistry studies in recent years have shown that its bark contains a variety of coumarin components, such as Esculin, Esculetin, Fraxin, and Fraxetol. The dried bark and bark of Aspergillus japonica have the effects of clearing away heat and dampness, improving eyesigh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 詹亚光刘孚婧齐凤慧尹静曾凡锁范桂枝
Owner NORTHEAST FORESTRY UNIVERSITY
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