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High yield baculovirus insect cell line and application thereof

A technology of insect cell line and baculovirus, which is applied to high-yield baculovirus insect cell lines and its application fields, can solve problems that have not been discovered, and achieve strong biological activity, high-efficiency expression, and high sensitivity

Active Publication Date: 2014-10-08
SOUTH CHINA UNITED VACCINE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many new cell lines have been established from different Lepidoptera insects, but no cell lines with excellent performance in protein expression have been found

Method used

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  • High yield baculovirus insect cell line and application thereof
  • High yield baculovirus insect cell line and application thereof
  • High yield baculovirus insect cell line and application thereof

Examples

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Embodiment 1

[0039] 1. Establishment of Super Sf-a cell line

[0040] Super Sf-a (Super Spodopterafrugiperda a) cell line was isolated from S.frugiperda Sf-9. The cell line is obtained from Sf-9 cells through multiple rounds of limiting dilution, and then using commercial serum-free insect suspension medium for conditional screening, and a cell bank that meets the requirements of the cell matrix for vaccine production has been established. The specific screening steps are as follows:

[0041] 1) The Sf-9 (Spodoptera frugiperda Sf-9) used for screening is derived from ATCC (ATCC CRL-1711). According to the certification materials (COA) provided by ATCC, the cells are derived from Texas A&M University (Texas A&M university) , passed 4 generations at Texas A&M University, passed 16 generations at ATCC, the inventor continued to pass 30 generations after purchasing and established a cell bank (sf-9 master cell bank, batch number 20130304), frozen in liquid nitrogen, and then One cell was rec...

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Abstract

The invention discloses a high yield baculovirus insect cell line Super Spodoptera frugiperda a (SuperSf-a) with an accession number of CCTCCNo.C2014100; the high yield baculovirus insect cell line has the ability of high yield production of baculovirus, can efficiently express an exogenous gene, has the characteristics of high baculovirus infection susceptibility and the like, an exogenous viral protein or a virus like particle product expressed by the cell line has a strong biological activity, and meets requirements of vaccine production; by exogenous virus detection, mycoplasma detection, sterility detection and exogenous factor detection, various indices of the cell line are in accordance with the requirements of cell matrix used for vaccine production; and the cell line can be used in baculovirus and vaccine production.

Description

technical field [0001] The invention relates to a new insect cell line, in particular to an insect cell line with high yield of baculovirus and its application. Background technique [0002] The practice that BEVS has been successfully used to express many foreign genes in the past 20 years has proved that there are currently 3 relatively good cell lines with good properties suitable for the research and production of AcMNPB and BmNPV recombinant protein expression. They are derived from The IPLB-SF21AE (Sf21) cell line from the ovary tissue of Fall Armyworm and its derivative Sf9, the BTI TN5B1-4 (High Five) cell line from the cabbage wok, and the BmN and its sublines from the ovary of the silkworm. None of these insect cell lines contain potentially deleterious transformation factors like many commonly used mammalian cell lines. Recently, Protein Science Company reported a new commercially licensed cell line (Express SF+), which is better than Sf9, and another commercial ...

Claims

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Application Information

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IPC IPC(8): C12N5/07C12N7/01C12N15/866A61K39/125A61K39/12A61K39/13A61K39/145A61P31/14A61P31/20A61P31/16C12R1/93
CPCY02A50/30
Inventor 彭涛安鸿王弋马书智尹海滨
Owner SOUTH CHINA UNITED VACCINE INST
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