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Kit for measuring high-density lipoprotein cholesterol

A high-density lipoprotein and kit technology, applied in the field of high-density lipoprotein cholesterol determination kits, can solve the problems of incomplete non-HDL masking inhibition, increased cost, poor practicability and the like

Active Publication Date: 2014-10-01
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The PEG modification method needs to modify the enzyme, which greatly reduces the activity of the enzyme and increases the cost; the specific antibody of the immunoseparation method is expensive and has many reaction steps, and the practicability is poor; the main key of the clearance method and the selective inhibition method is surface activity The choice of agent, but the use of surfactant is more difficult, the masking inhibition of non-HDL is not complete, and the release of HDL is not complete

Method used

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  • Kit for measuring high-density lipoprotein cholesterol
  • Kit for measuring high-density lipoprotein cholesterol
  • Kit for measuring high-density lipoprotein cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A specific detection kit for cholesterol in high-density lipoprotein (see Table 30 for the detection results). Wherein, R1 comprises following components (table 3):

[0051] table 3

[0052]

[0053] R2 includes the following components (Table 4):

[0054] Table 4

[0055]

[0056]

Embodiment 2

[0058] A specific detection kit for cholesterol in high-density lipoprotein (see Table 30 for the detection results). Wherein, R1 comprises following components (table 5):

[0059] table 5

[0060]

[0061] R2 includes the following components (Table 6):

[0062] Table 6

[0063]

Embodiment 3

[0065] A specific detection kit for cholesterol in high-density lipoprotein (see Table 30 for the detection results). Wherein, R1 comprises following components (table 7):

[0066] Table 7

[0067]

[0068] R2 includes the following components (Table 8):

[0069] Table 8

[0070]

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PUM

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Abstract

The invention relates to a kit for measuring high-density lipoprotein cholesterol. The kit comprises a first reagent and a second reagent, wherein the first reagent comprises a surface active agent I used for inhibiting the dissolution of non-high-density lipoprotein (NHDL), and a surface active agent II used for dissolving high-density lipoprotein (HDL); after the surface active agent I is mixed with the surface active agent II, the value of hydrophile-lipophile balance (HLB) is within the range of 13-14; the volume ratio of the surface active agent I to the surface active agent II in the first reagent is (1:30)-(1:1). Due to the synergistic effect of components, the stability of the kit is improved, and the HDL can be specifically released; furthermore, the dissociation of the NHDL can be specifically masked and inhibited, the interference of other components in serum can be eliminated, and the accuracy of measurement is guaranteed.

Description

technical field [0001] The invention relates to the technical field of measuring cholesterol, in particular to a kit for measuring high-density lipoprotein cholesterol. Background technique [0002] In addition to free cholesterol, most of the cholesterol in human blood is combined with lipoproteins such as very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) to form lipoprotein cholesterol. Among them, HDL, also known as a1 lipoprotein, is relatively rich in phospholipids, and its content in serum is about 300 mg / dl. The protein part, A-I is about 75%, and A-II is about 20%. HDL receives cholesterol from various tissues including the arterial wall, transports it to the liver through direct reverse transport or indirect reverse transport, and then clears it out of the blood. Therefore, HDL is involved in the removal of cholesterol accumulated in cells. Therefore, high-density lipoprotein cholesterol (HDL-C) is a risk preven...

Claims

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Application Information

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IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 袁锦云时敏韦锐叶强张华杰
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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